anti-Human FAS antibody for Functional Studies

Recommended FAS Antibody (supplied by: Log in to see )

Antigen
Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
  • AI196731
  • APO1
  • APT1
  • CD95
  • TNFR6
  • Tnfrsf6
  • lpr
  • TNFRSF6
  • ALPS1A
  • APO-1
  • FAS1
  • FASTM
  • A630082H08Rik
  • FAS
  • Fas (TNF receptor superfamily member 6)
  • Fas (TNF receptor superfamily, member 6)
  • Fas cell surface death receptor
  • fatty acid synthase
  • Fas
  • FAS
  • Fasn
Reactivity
Baboon, Cynomolgus, Dog (Canine), Human, Pig (Porcine), Rhesus Monkey
646
113
99
14
11
10
5
5
3
3
2
2
2
1
Host
Mouse
414
303
24
5
4
3
3
Clonality
Monoclonal
Conjugate
This FAS antibody is un-conjugated
73
63
51
29
15
8
7
7
5
5
5
4
4
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
334
318
210
146
113
85
61
54
52
45
23
18
12
7
7
6
5
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
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Catalog No. ABIN1177357
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967518 Func FACS ELISA WB Mouse IgG1 Log in to see G254-274 2
1 ABIN94250 Func FACS Mouse IgM Log in to see UT-1 4
1 ABIN265936 Func FACS Mouse IgM Log in to see UT-1
1 ABIN1106615 Func FACS Mouse IgG1 Log in to see B-D29
1 ABIN1106614 Func IHC (fro) FACS Mouse IgG2a Log in to see B-G27
1 ABIN371578 Func FACS IHC (fro) IP Mouse IgG1 Log in to see DX2 1
1 ABIN371584 Func FACS IHC (fro) IP Mouse IgG2a Log in to see DX3 1
1 ABIN187262 Func FACS IHC (fro) IF Mouse IgM Log in to see IPO-4
1 ABIN120703 Func FACS Mouse IgM Log in to see UT-1
1 ABIN187285 Func FACS IHC (fro) Mouse IgG1 Log in to see APO-1-1
1 ABIN2689464 FM Func FACS Mouse IgM, kappa Log in to see EOS9-1 3
1 ABIN206910 Func FACS Mouse IgM Log in to see UT-1
1 ABIN228061 Func FACS IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see
1 ABIN228064 Func FACS Biotin Mouse IgG2b Log in to see
1 ABIN228060 Func FACS IHC (fro) IHC (p) Biotin Mouse IgG1 Log in to see
1 ABIN228065 Func FACS FITC Mouse IgG2b Log in to see
1 ABIN371577 Func FACS IHC (fro) IP Mouse IgG1 Log in to see DX2
1 ABIN2749190 Func Mouse IgM Log in to see EOS9-1 4
1 ABIN200905 Func FACS IP WB Mouse IgG3 Log in to see
1 ABIN209539 Func FACS IP Mouse IgG1 Extracellular Domain Log in to see

General

Antigen Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
Reactivity Baboon, Cynomolgus, Dog (Canine), Human, Pig (Porcine), Rhesus Monkey
(646), (113), (99), (14), (11), (10), (5), (5), (3), (3), (2), (2), (2), (1)
Host Mouse
(414), (303), (24), (5), (4), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FAS antibody is un-conjugated
(73), (63), (51), (29), (15), (8), (7), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS)
(334), (318), (210), (146), (113), (85), (61), (54), (52), (45), (23), (18), (12), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FAS Antibody

Target Details FAS Application Details Handling ProductDetails: References for anti-FAS antibody (ABIN1177357) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen Human CD95-transfected L ceils
Clone DX2
Isotype IgG1, kappa

Target Details FAS

Product Details anti-FAS Antibody Application Details Handling ProductDetails: References for anti-FAS antibody (ABIN1177357) Images back to top
Antigen
Alternative Name CD95 (FAS Antibody Abstract)
Background Reacts with the 45 kDa transmembrane cell surface Fas or Apo-1 antigen (designated CD95 at the Fifth HLDA Workshop) which is expressed on a variety of normal and neoplastic cells. The Fas/Apo-1 antigen is a polypeptide, which plays a role in the programmed sequence of events leading to cell death, termed apoptosis. The DX2 clone specifically reacts with murine L cells, murine L1210 leukemia cells and murine P815 mastocytoma cells transfected with human Fas cDNA but not with untransfected parental cell lines. Cross-linking with DX2 delivers an apoptotic signal indicating that DX2 recognizes a functional epitope of the CD95 antigen.
Synonyms: Fas/APO-1
Research Area Surface Receptors of Immune Cells
Pathways p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FAS Antibody Target Details FAS Handling ProductDetails: References for anti-FAS antibody (ABIN1177357) Images back to top
Protocol 1. Maintain cells in culture with 10% FBS medium, change the medium one day before starting the induction of apoptosis. Harvest and pellet the cells, resuspend in 10% FBS medium at a density of 0.5-1.0 x 10^6 cells/ml.
2. In a 96-well microtiter plate, add 2.5 µg/50 µl CD95 NA/LE™, 0.5 µg/50 µl rProt G, 0.5-1.0 x 10^5 cells and 10% FBS medium to a total volume of 200 µl. Negative controls should consist of: 1) 0.5-1.0 x 10^5 cells with 10% FBS medium alone (200 µl total volume), and 2) 0.5-1.0 x 10^5 cells with 0.5 µg/50 µl rProtG and 10% FBS medium.
3. Incubate the 96-well plate at 37°C, 5% CO2, for 12-24 hours. Traditionally, apoptosis has been observed by light microscopy, MTT, or gel electrophoresis (DNA fragmentation). Investigators may be interested in several products offered by BD Biosciences that may be used for the detection of apoptotic cells by flow cytometry: ApoDirect™ Kit (Cat. No. 556381), ApoBRDU™ Kit (Cat. No. 556405) and Annexin V-FITC (Cat. No. 556420, Cat. No. 556419). Because these methods vary in sensitivity, it may be necessary to titer the rProtG and/or CD95 NA/LE™ to obtain optimal results.
Notes: The suggestions given in this procedure are based on conditions that were found to be optimal for the induction of apoptosis by anti-human CD95 (Fas). Studies have shown that the addition of protein G can significantly enhance the efficiency of DX2 in this type of functional assay. It is important to note that there is a great deal of variation between cell lines in the level of apoptosis that can be induced through the Fas receptor. It has been reported that, although some cells express the Fas antigen, they do not necessarily undergo Fas-mediated apoptosis (i.e., the Fas antigen expressed may be anti-Fas sensitive or insensitive). Daudi, Jurkat, and HPB-ALL cells are good positive controls as they are strongly induced by anti-Fas to undergo apoptosis.
Restrictions For Research Use only

Handling

Product Details anti-FAS Antibody Target Details FAS Application Details ProductDetails: References for anti-FAS antibody (ABIN1177357) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

ProductDetails: References for anti-FAS antibody (ABIN1177357)

Product Details anti-FAS Antibody Target Details FAS Application Details Handling Images back to top
Product cited in:

Cifone, De Maria, Roncaioli, Rippo, Azuma, Lanier, Santoni, Testi: "Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase." in: The Journal of experimental medicine, Vol. 180, Issue 4, pp. 1547-52, 1994

Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, 1991

Images

Product Details anti-FAS Antibody Target Details FAS Application Details Handling ProductDetails: References for anti-FAS antibody (ABIN1177357) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody (ABIN1177357) anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody