anti-Human FAS antibody for Functional Studies

Recommended FAS Antibody (supplied by: Log in to see )

Antigen
Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
  • AI196731
  • APO1
  • APT1
  • CD95
  • TNFR6
  • Tnfrsf6
  • lpr
  • TNFRSF6
  • ALPS1A
  • APO-1
  • FAS1
  • FASTM
  • A630082H08Rik
  • FAS
  • Fas (TNF receptor superfamily member 6)
  • Fas (TNF receptor superfamily, member 6)
  • Fas cell surface death receptor
  • fatty acid synthase
  • Fas
  • FAS
  • Fasn
Reactivity
Human
640
112
99
15
11
10
6
6
3
3
3
3
2
1
Host
Mouse
413
298
23
5
4
3
3
Clonality
Monoclonal
Conjugate
This FAS antibody is un-conjugated
73
63
51
29
15
8
7
7
5
5
5
4
4
4
4
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
333
310
206
145
112
84
60
52
52
45
23
18
12
7
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN967518
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1177357 Func FACS Mouse IgG1, kappa Log in to see DX2 2
1 ABIN94250 Func FACS Mouse IgM Log in to see UT-1 4
1 ABIN1106614 Func IHC (fro) FACS Mouse IgG2a Log in to see B-G27
1 ABIN1106615 Func FACS Mouse IgG1 Log in to see B-D29
1 ABIN371584 Func FACS IHC (fro) IP Mouse IgG2a Log in to see DX3 1
1 ABIN371578 Func FACS IHC (fro) IP Mouse IgG1 Log in to see DX2 1
1 ABIN187300 EIA Func IHC (fro) IP WB Mouse IgG1 Log in to see 5F-9
1 ABIN265936 Func FACS Mouse IgM Log in to see UT-1
1 ABIN187262 Func FACS IHC (fro) IF Mouse IgM Log in to see IPO-4
1 ABIN187285 Func FACS IHC (fro) Mouse IgG1 Log in to see APO-1-1
1 ABIN120703 Func FACS Mouse IgM Log in to see UT-1
1 ABIN2689464 FM Func FACS Mouse IgM, kappa Log in to see EOS9-1 3
1 ABIN206910 Func FACS Mouse IgM Log in to see UT-1
1 ABIN371577 Func FACS IHC (fro) IP Mouse IgG1 Log in to see DX2
1 ABIN228060 Func FACS IHC (fro) IHC (p) Biotin Mouse IgG1 Log in to see
1 ABIN228064 Func FACS Biotin Mouse IgG2b Log in to see
1 ABIN228061 Func FACS IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see
1 ABIN228065 Func FACS FITC Mouse IgG2b Log in to see
1 ABIN200905 Func FACS IP WB Mouse IgG3 Log in to see
1 ABIN371583 Func FACS IHC (fro) IP Mouse IgG2a Log in to see DX3

Top referenced anti-FAS antibody for Functional Studies

Similar anti-FAS Antibodies

Application / Reactivity Human
Activation (Act) 3 Antibodies
Apoptosis Blocking (AB) 3 Antibodies
Apoptosis Induction (AI) 2 Antibodies
Biochemical Assay (BCA) 1 Antibodies
Blocking Antibody (Inhibition) 5 Antibodies
Blocking Reagent (BR) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 18 Antibodies
ELISA 181 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 299 Antibodies
Fluorescence Microscopy (FM) 1 Antibodies
Functional Studies (Func) 46 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 11 Antibodies

General

Antigen Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
Reactivity Human
(640), (112), (99), (15), (11), (10), (6), (6), (3), (3), (3), (3), (2), (1)
Host Mouse
(413), (298), (23), (5), (4), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FAS antibody is un-conjugated
(73), (63), (51), (29), (15), (8), (7), (7), (5), (5), (5), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(333), (310), (206), (145), (112), (84), (60), (52), (52), (45), (23), (18), (12), (7), (7), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-FAS Antibody

Target Details FAS Application Details Handling ProductDetails: References for anti-FAS antibody (ABIN967518) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant Human Fas
Clone G254-274
Isotype IgG1

Target Details FAS

Product Details anti-FAS Antibody Application Details Handling ProductDetails: References for anti-FAS antibody (ABIN967518) Images back to top
Antigen
Alternative Name CD95 (FAS Antibody Abstract)
Background Programmed cell death (apoptosis) may be induced in response to a variety of cytotoxic stimuli, including activation of surface receptors. An area of particular interest has been the induction of apoptosis by the receptor-ligand pair: Fas (CD95) and Fas Ligand (FasL). Fas is an ~45 kD cell surface protein which belongs to the the TNF (tumor necrosis factor receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL, a member of the TNF ligand family, is expressed on activated T and NK cells. FasL initiates signaling at the cell surface by aggregation of individual Fas recpetors via binding to the multivalent ligand. Antibodies which selectively activate Fas can be used in vitro to mimic the apoptotic response associated with FasL. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development. Clone G254-274 recognizes human Fas. A soluble form of recombinant human Fas (lacking the Fas transmembrane domain) was used as immunogen.
Synonyms: Fas/APO-1
Molecular Weight 45 kDa
Research Area Immunology, Innate Immunity, Cytokines, CD Antigens, Apoptosis/Necrosis, Death Receptors, Cancer, Growth Factors, Surface Receptors of Immune Cells
Pathways p53 Signaling, Apoptosis, TCR Signaling, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-FAS Antibody Target Details FAS Handling ProductDetails: References for anti-FAS antibody (ABIN967518) Images back to top
Application Notes Applications include western blot analysis (1-2µg/ml). Clone G254-274 has also been tested in antibody development for ELISA where G254-274 has been used as a capture antibody paired with the biotin mouse anti-human Fas clone DX2 (MN 555672) as the detection antibody. Clone G254-274 is not suggested for flow cytometric analysis or for use in functional apoptosis assays.
Comment

Related Products: ABIN967460, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-FAS Antibody Target Details FAS Application Details ProductDetails: References for anti-FAS antibody (ABIN967518) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

ProductDetails: References for anti-FAS antibody (ABIN967518)

Product Details anti-FAS Antibody Target Details FAS Application Details Handling Images back to top
Product cited in:

Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, 1995

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, 1994

Images

Product Details anti-FAS Antibody Target Details FAS Application Details Handling ProductDetails: References for anti-FAS antibody (ABIN967518) back to top
Supplier Images
Western Blotting (WB) image for anti-FAS antibody (Fas (TNF Receptor Superfamily, Member 6)) (ABIN967518) Western blot analysis of recombinant FasÄTM. Purified mouse anti-human Fas antibody (...
 image for anti-FAS antibody (Fas (TNF Receptor Superfamily, Member 6)) (ABIN967518) anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody (Image 2)