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anti-Mouse (Murine) FAS antibody for Cytotoxicity Test

Recommended FAS Antibody (supplied by: Log in to see )

Antigen
Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
  • A630082H08Rik
  • AI196731
  • ALPS1A
  • APO-1
  • APO1
  • APT1
  • CD95
  • FAS
  • FAS1
  • FASTM
  • lpr
  • TNFR6
  • Tnfrsf6
  • TNFRSF6
Reactivity
Mouse (Murine)
652
100
81
11
7
5
4
3
3
3
2
2
1
1
Host
Armenian Hamster
427
293
17
6
4
3
2
Clonality
Monoclonal
Conjugate
This FAS antibody is un-conjugated
74
64
51
33
16
8
7
6
4
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
Application
Cytotoxicity Test (CyTox), Flow Cytometry (FACS), Immunoprecipitation (IP)
339
295
213
130
103
78
61
55
49
44
23
19
12
9
7
5
4
4
3
3
3
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN1177304
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008784314 ABIN2689461 CyTox FACS IP Armenian Hamster IgG2, lambda Log in to see Jo2 5

Top referenced anti-FAS antibody for Cytotoxicity Test

Similar anti-FAS Antibodies

Application / Reactivity Mouse (Murine)
Cytotoxicity Test (CyTox) 2 Antibodies
ELISA 15 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 24 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Neutralization (Neut) 1 Antibodies
Western Blotting (WB) 50 Antibodies

General

Antigen Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
Reactivity Mouse (Murine)
(652), (100), (81), (11), (7), (5), (4), (3), (3), (3), (2), (2), (1), (1)
Host Armenian Hamster
(427), (293), (17), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FAS antibody is un-conjugated
(74), (64), (51), (33), (16), (8), (7), (6), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Cytotoxicity Test (CyTox), Flow Cytometry (FACS), Immunoprecipitation (IP)
(339), (295), (213), (130), (103), (78), (61), (55), (49), (44), (23), (19), (12), (9), (7), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-FAS Antibody

Target Details FAS Application Details Handling References for anti-FAS Antibody (ABIN1177304) Images
Brand BD Pharmingen™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Sterility 0.2 μm filtered
Endotoxin Level Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
Immunogen WR19L mouse lymphoma cells transformed with recombinant mouse Fas
Clone Jo2
Isotype IgG2, lambda

Target Details FAS

Product Details anti-FAS Antibody Application Details Handling References for anti-FAS Antibody (ABIN1177304) Images back to top
Antigen
Alternative Name CD95 (FAS Antibody Abstract)
Background Fas antigen, CD95, is a 45 kDa cell-surface protein which can mediate apoptosis. It belongs to the TNF (tumor necrosis factor)/NGF receptor family. Expression of Fas has been described in the thymus, liver, heart, lung and ovary. Fas plays an important role in the apoptotic process that takes place during development. Monoclonal antibodies recognizing Fas such as Jo2 have cytolytic activity on cells expressing Fas. The cell death stimulated by Fas antibodies is characteristic of apoptosis and suggests that the lethal effects are a result of interaction of antibody with a functional Fas antigen as opposed to complement-mediated lysis. The Jo2 antibody recognizes mouse Fas. The Jo2 antibody shows cytolytic activity against cell lines expressing mouse Fas by inducing apoptosis. Intraperitoneal injections of Jo2 mAb have been shown to kill mice and induce apoptotic hepatocyte death. Jo2 mAb has been reported to immunoprecipitate mouse Fas as a 45 kDa band from W4 cells. W4 cells are WR19L mouse lymphoma cells transformed with mouse Fas. The difference between the observed MW of Fas and that deduced from its amino acid sequence (Mr 34,971) may be due to glycosylation.
Synonyms: Fas/APO-1
Molecular Weight 45 kDa
Research Area Surface Receptors of Immune Cells
Pathways p53 Signaling, Apoptosis, TCR Signaling

Application Details

Product Details anti-FAS Antibody Target Details FAS Handling References for anti-FAS Antibody (ABIN1177304) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-FAS Antibody Target Details FAS Application Details References for anti-FAS Antibody (ABIN1177304) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative Azide free
Storage 4 °C
Storage Comment Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-FAS Antibody (ABIN1177304)

Product Details anti-FAS Antibody Target Details FAS Application Details Handling Images back to top
Product cited in:

Enari, Hug, Nagata: "Involvement of an ICE-like protease in Fas-mediated apoptosis." in: Nature, Vol. 375, Issue 6526, pp. 78-81, 1995

Nagata: "Apoptosis regulated by a death factor and its receptor: Fas ligand and Fas." in: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 345, Issue 1313, pp. 281-7, 1995

Nagata: "Fas and Fas ligand: a death factor and its receptor." in: Advances in immunology, Vol. 57, pp. 129-44, 1995

Ni, Tomita, Matsuda, Ichihara, Ishimura, Ogasawara, Nagata: "Fas-mediated apoptosis in primary cultured mouse hepatocytes." in: Experimental cell research, Vol. 215, Issue 2, pp. 332-7, 1995

Ogasawara, Suda, Nagata: "Selective apoptosis of CD4+CD8+ thymocytes by the anti-Fas antibody." in: The Journal of experimental medicine, Vol. 181, Issue 2, pp. 485-91, 1995

Yang, Merćep, Ware, Ashwell: "Fas and activation-induced Fas ligand mediate apoptosis of T cell hybridomas: inhibition of Fas ligand expression by retinoic acid and glucocorticoids." in: The Journal of experimental medicine, Vol. 181, Issue 5, pp. 1673-82, 1995

Hiromatsu, Aoki, Makino, Matsumoto, Mizuochi, Gotoh, Nomoto, Ogasawara, Nagata, Yoshikai: "Increased Fas antigen expression in murine retrovirus-induced immunodeficiency syndrome, MAIDS." in: European journal of immunology, Vol. 24, Issue 10, pp. 2446-51, 1994

Kägi, Vignaux, Ledermann, Bürki, Depraetere, Nagata, Hengartner, Golstein: "Fas and perforin pathways as major mechanisms of T cell-mediated cytotoxicity." in: Science (New York, N.Y.), Vol. 265, Issue 5171, pp. 528-30, 1994

Ogasawara, Watanabe-Fukunaga, Adachi, Matsuzawa, Kasugai, Kitamura, Itoh, Suda, Nagata: "Lethal effect of the anti-Fas antibody in mice." in: Nature, Vol. 364, Issue 6440, pp. 806-9, 1993

Images

Product Details anti-FAS Antibody Target Details FAS Application Details Handling References for anti-FAS Antibody (ABIN1177304) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FAS antibody (Fas (TNF Receptor Superfamily, Member 6)) (ABIN1177304) anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody