You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human GADD45A antibody for Western Blotting

Recommended GADD45A Antibody (supplied by: Log in to see )

Antigen
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
  • AA545191
  • ddit1
  • Ddit1
  • DDIT1
  • Gadd45
  • GADD45
  • gadd45
  • gadd45a
  • GADD45A
  • MGC53491
  • zgc:91795
Epitope
C-Term
16
13
8
6
4
2
2
2
1
1
1
1
1
Reactivity
Human
79
29
28
Host
Rabbit
65
15
Clonality
Polyclonal
Conjugate
This GADD45A antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
58
20
11
11
5
4
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN453659
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.1205588 ABIN389281 FACS WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 4
0.1205588 ABIN650724 FACS WB Rabbit Ig AA 133-165, C-Term Log in to see Polyclonal 5
0.1205588 ABIN2788360 WB Rabbit C-Term Log in to see Polyclonal
0.1205588 ABIN2169518 WB Mouse IgG1 Log in to see 6C3-G3-E9
0.1205588 ABIN3042713 WB Rabbit IgG AA 129-143, C-Term Log in to see Polyclonal
0.1205588 ABIN1872769 WB Rabbit IgG Log in to see Polyclonal
0.1205588 ABIN1872770 IHC WB Rabbit IgG Log in to see Polyclonal
0.1205588 ABIN2437252 FACS ELISA WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.1205588 ABIN2439390 ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.1205588 ABIN2404654 IHC WB Rabbit IgG Log in to see Polyclonal
0.1205588 ABIN514931 WB Mouse AA 1-165, full length Log in to see Polyclonal
0.1205588 ABIN3198080 IC IF WB Rabbit Log in to see Polyclonal
0.1205588 ABIN926429 WB Rabbit C-Term Log in to see Polyclonal
0.1205588 ABIN4313173 WB Mouse IgG2b Log in to see 1C9
0.1205588 ABIN2437251 WB Rabbit C-Term Log in to see Polyclonal
0.1205588 ABIN3022564 IHC WB Rabbit Log in to see Polyclonal
0.1205588 ABIN3015354 WB Rabbit Log in to see Polyclonal
0.1205588 ABIN4894135 WB Rabbit C-Term Log in to see Polyclonal
0.1205588 ABIN3031106 WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011963103 ABIN357951 EIA WB Rabbit Ig N-Term Log in to see Polyclonal 2

Top referenced anti-GADD45A antibody for Western Blotting

Similar anti-GADD45A Antibodies

Application / Reactivity Human
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 59 Antibodies

General

Antigen Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
Epitope C-Term
(16), (13), (8), (6), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(79), (29), (28)
Host Rabbit
(65), (15)
Clonality
Polyclonal
Conjugate This GADD45A antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(58), (20), (11), (11), (5), (4), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-GADD45A Antibody

Target Details GADD45A Application Details Handling References for anti-GADD45A Antibody (ABIN453659) Images
Specificity This antibody reacts to GADD45A.
Purification Saturated Ammonium Sulfate (SAS) precipitation
Immunogen KLH conjugated synthetic peptide selected from the C-terminal region of human GADD45A

Target Details GADD45A

Product Details anti-GADD45A Antibody Application Details Handling References for anti-GADD45A Antibody (ABIN453659) Images back to top
Antigen
Alternative Name GADD45A (GADD45A Antibody Abstract)
Background GADD45A responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The GADD45A gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms.Synonyms: DDIT-1, DDIT1, DNA damage-inducible transcript 1 protein, GADD45, Growth arrest and DNA damage-inducible protein GADD45 alpha
Gene ID 1647
NCBI Accession NP_001915
UniProt P24522
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-GADD45A Antibody Target Details GADD45A Handling References for anti-GADD45A Antibody (ABIN453659) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details References for anti-GADD45A Antibody (ABIN453659) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-GADD45A Antibody (ABIN453659)

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling Images back to top
Background publications

Li, Morales, Hwang, Wagner, Boothman: "DNA mismatch repair-dependent activation of c-Abl/p73alpha/GADD45alpha-mediated apoptosis." in: The Journal of biological chemistry, Vol. 283, Issue 31, pp. 21394-403, 2008

Zhu, Ren, Korchin, Lahat, Dicker, Lu, Mills, Pollock, Lev: "Soft tissue sarcoma cells are highly sensitive to AKT blockade: a role for p53-independent up-regulation of GADD45 alpha." in: Cancer research, Vol. 68, Issue 8, pp. 2895-903, 2008

Al-Romaih, Sadikovic, Yoshimoto, Wang, Zielenska, Squire: "Decitabine-induced demethylation of 5' CpG island in GADD45A leads to apoptosis in osteosarcoma cells." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 5, pp. 471-80, 2008

Kearsey, Coates, Prescott, Warbrick, Hall: "Gadd45 is a nuclear cell cycle regulated protein which interacts with p21Cip1." in: Oncogene, Vol. 11, Issue 9, pp. 1675-83, 1995

Images

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling References for anti-GADD45A Antibody (ABIN453659) back to top
Supplier Images
Western Blotting (WB) image for anti-GADD45A antibody (Growth Arrest and DNA-Damage-Inducible, alpha) (C-Term) (ABIN453659) Western blot analysis of GADD45A Antibody (C-term) in Y79 cell line lysates (35ug/lan...