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|Application / Reactivity||Human||Rat (Rattus)|
|Western Blotting (WB)||56 Antibodies||11 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||3 Antibodies|
|Immunohistochemistry (IHC)||11 Antibodies||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Flow Cytometry (FACS)||5 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|ELISA||19 Antibodies||5 Antibodies|
|Antigen||Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies|
|Epitope||AA 129-143, C-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This GADD45A antibody is un-conjugated Alternatives|
Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-GADD45A AntibodyTarget Details GADD45A Application Details Handling Images
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Rabbit IgG polyclonal antibody for Growth arrest and DNA damage-inducible protein GADD45 alpha(GADD45A) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: growth arrest and DNA-damage-inducible, alpha
Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A(129-143aa NPHSSQWKDPALSQL), identical to the related mouse and rat sequences.|
Target Details GADD45AProduct Details anti-GADD45A Antibody Application Details Handling Images back to top
|Alternative Name||GADD45A (GADD45A Antibody Abstract)|
|Background||Growth arrest and DNA-damage-inducible protein GADD45 alpha(GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Papathanasiou et al.(1991) demonstrated that the GADD45 gene is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.|
|Research Area||Chromatin and Nuclear Signaling, DNA/RNA|
|Pathways||p53 Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-GADD45A Antibody Target Details GADD45A Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GADD45A Antibody Target Details GADD45A Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling back to top