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anti-Rat (Rattus) GADD45A antibody for Western Blotting

Recommended GADD45A Antibody (supplied by: Log in to see )

Antigen
Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
  • AA545191
  • ddit1
  • Ddit1
  • DDIT1
  • Gadd45
  • GADD45
  • gadd45
  • gadd45a
  • GADD45A
  • MGC53491
  • zgc:91795
Epitope
AA 129-143, C-Term
15
13
10
7
4
2
2
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
79
29
27
Host
Rabbit
66
14
Clonality
Polyclonal
Conjugate
This GADD45A antibody is un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
58
19
11
11
6
4
2
1
1
1
Supplier
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Catalog No. ABIN3042713
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN1872770 IHC WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN1872769 WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN2404654 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN3198080 IC IF WB Rabbit Log in to see Polyclonal
0.0011728023 ABIN1086822 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1086821 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2417461 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2986261 WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN2990358 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN3031106 WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-GADD45A Antibodies

Application / Reactivity Human Rat (Rattus)
Western Blotting (WB) 59 Antibodies 11 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 11 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
ELISA 19 Antibodies 5 Antibodies

General

Antigen Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A) Antibodies
Epitope AA 129-143, C-Term
(15), (13), (10), (7), (4), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(79), (29), (27)
Host Rabbit
(66), (14)
Clonality
Polyclonal
Conjugate This GADD45A antibody is un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(58), (19), (11), (11), (6), (4), (2), (1), (1), (1)
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Product Details anti-GADD45A Antibody

Target Details GADD45A Application Details Handling Images
Sequence NPHSSQWKDP ALSQL
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for Growth arrest and DNA damage-inducible protein GADD45 alpha(GADD45A) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: growth arrest and DNA-damage-inducible, alpha
Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GADD45A(129-143aa NPHSSQWKDPALSQL), identical to the related mouse and rat sequences.
Isotype IgG

Target Details GADD45A

Product Details anti-GADD45A Antibody Application Details Handling Images back to top
Antigen
Alternative Name GADD45A (GADD45A Antibody Abstract)
Background Growth arrest and DNA-damage-inducible protein GADD45 alpha(GADD45A) is a protein that in humans is encoded by the GADD45A gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. Papathanasiou et al.(1991) demonstrated that the GADD45 gene is located on 1p34-p12. Sequence analysis of human and hamster cDNA clones demonstrated that the gene has been highly conserved and encodes a novel 165-amino acid polypeptide. Northern blot analysis detected moderate expression of a 1.4-kb GADD45A transcript in heart, skeletal muscle, and kidney, with little or no expression in brain, placenta, lung, liver, and pancreas. Gadd45a promotes epigenetic gene activation by repair-mediated DNA demethylation.
UniProt P24522
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-GADD45A Antibody Target Details GADD45A Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions For Research Use only

Handling

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-GADD45A Antibody Target Details GADD45A Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-GADD45A antibody (Growth Arrest and DNA-Damage-Inducible, alpha) (AA 129-143) (ABIN3042713) Anti-GADD45A antibody, Western blotting Lane 1: MM453 Cell Lysate Lane 2: MM231 Cell ...