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anti-Human GTPase NRas antibody for Enzyme Immunoassay

Recommended GTPase NRas Antibody (supplied by: Log in to see )

Antigen
GTPase NRas (NRAS) Antibodies
  • ALPS4
  • AV095280
  • fj99a10
  • H-RAS
  • HRAS
  • N-RAS
  • N-ras
  • NRAS
  • NRAS1
  • NS6
  • wu:fj99a10
  • zgc:100761
  • zras-b1
Epitope
C-Term
21
10
10
7
4
3
3
3
2
1
1
1
1
Reactivity
Human
94
21
15
4
1
Host
Rabbit
40
34
20
Clonality
Polyclonal
Conjugate
This GTPase NRas antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
88
61
30
26
22
21
19
4
2
1
1
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Catalog No. ABIN360039
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN360038 EIA FACS IF WB Rabbit Ig N-Term Log in to see Polyclonal 2
0.0008867729 ABIN781533 EIA WB Mouse IgG2a Log in to see AT2G9 3
0.0008867729 ABIN372821 EIA IHC (p) WB Goat IgG Internal Region Log in to see Polyclonal
0.0008867729 ABIN2727604 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-GTPase NRas antibody for Enzyme Immunoassay

Similar anti-GTPase NRas Antibodies

Application / Reactivity Human
ELISA 61 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 27 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 90 Antibodies

General

Antigen GTPase NRas (NRAS) Antibodies
Epitope C-Term
(21), (10), (10), (7), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Human
(94), (21), (15), (4), (1)
Host Rabbit
(40), (34), (20)
Clonality
Polyclonal
Conjugate This GTPase NRas antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(88), (61), (30), (26), (22), (21), (19), (4), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-GTPase NRas Antibody

Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN360039) Images
Specificity This antibody reacts to NRAS.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NRAS.
Isotype Ig

Target Details GTPase NRas

Product Details anti-GTPase NRas Antibody Application Details Handling References for anti-GTPase NRas Antibody (ABIN360039) Images back to top
Antigen
Alternative Name NRAS (NRAS Antibody Abstract)
Background NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
Gene ID 4893, 9606
UniProt P01111
Research Area Cancer
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Handling References for anti-GTPase NRas Antibody (ABIN360039) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details References for anti-GTPase NRas Antibody (ABIN360039) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-GTPase NRas Antibody (ABIN360039)

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling Images back to top
Background publications

Smalley, Contractor, Nguyen, Xiao, Edwards, Muthusamy, King, Flaherty, Bosenberg, Herlyn, Nathanson: "Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression." in: Cancer research, Vol. 68, Issue 14, pp. 5743-52, 2008

Banerji, Affolter, Judson, Marais, Workman: "BRAF and NRAS mutations in melanoma: potential relationships to clinical response to HSP90 inhibitors." in: Molecular cancer therapeutics, Vol. 7, Issue 4, pp. 737-9, 2008

Images

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN360039) back to top
Supplier Images
 image for anti-GTPase NRas antibody (NRAS) (C-Term) (ABIN360039) anti-GTPase NRas (NRAS) (C-Term) antibody
 image for anti-GTPase NRas antibody (NRAS) (C-Term) (ABIN360039) anti-GTPase NRas (NRAS) (C-Term) antibody (Image 2)