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anti-Human GTPase NRas antibody for Flow Cytometry

Recommended GTPase NRas Antibody (supplied by: Log in to see )

Antigen
GTPase NRas (NRAS) Antibodies
  • NRAS
  • AV095280
  • N-ras
  • ALPS4
  • NRAS1
  • NS6
  • N-RAS
  • fj99a10
  • wu:fj99a10
  • zgc:100761
  • zras-b1
  • H-RAS
  • HRAS
  • GTPase NRas
  • African clawed frog N-ras
  • neuroblastoma ras oncogene
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • Tsp_01117
  • n-ras
  • Nras
  • NRAS
  • nras
Epitope
AA 147-179
23
9
8
7
6
5
4
4
2
1
1
1
1
1
1
Reactivity
Human
98
28
18
6
3
3
2
2
2
2
2
1
Host
Rabbit
44
34
20
Clonality
Polyclonal
Conjugate
This GTPase NRas antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
85
56
28
23
20
19
18
5
2
1
1
1
Supplier
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Catalog No. ABIN3031131
$ 411.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.528704 ABIN3031130 FACS IF ELISA WB Rabbit Ig Fraction AA 72-101 Log in to see Polyclonal
1 ABIN360038 EIA FACS IF WB Rabbit Ig N-Term Log in to see Polyclonal 2
1 ABIN392152 FACS IF WB Rabbit Ig AA 72-101, N-Term Log in to see Polyclonal 1
1 ABIN392153 FACS IHC (p) WB Rabbit Ig AA 147-179, C-Term Log in to see Polyclonal
1 ABIN4904578 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4340648 ELISA FACS ICC IF WB Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340649 ELISA FACS ICC IF WB Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340657 FACS Allophycocyanin Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340661 ELISA FACS ICC IF WB DyLight 680 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340668 ELISA FACS ICC IF WB DyLight 405 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340664 ELISA FACS ICC IF WB PerCP Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340666 ELISA FACS ICC IF WB DyLight 550 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340667 ELISA FACS ICC IF WB DyLight 350 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340653 ELISA FACS ICC IF WB Alexa Fluor 405 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340654 ELISA FACS ICC IF WB Alexa Fluor 488 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340656 ELISA FACS ICC IF WB Alexa Fluor 700 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340659 ELISA FACS ICC IF WB DyLight 650 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340665 FACS PE Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340663 ELISA FACS ICC IF WB DyLight 755 Mouse IgG2a kappa AA 1-186 Log in to see 2G9
1 ABIN4340655 ELISA FACS ICC IF WB Alexa Fluor 647 Mouse IgG2a kappa AA 1-186 Log in to see 2G9

Top referenced anti-GTPase NRas antibody for Flow Cytometry

Similar anti-GTPase NRas Antibodies

Application / Reactivity Human
ELISA 57 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 86 Antibodies

General

Antigen GTPase NRas (NRAS) Antibodies
Epitope AA 147-179
(23), (9), (8), (7), (6), (5), (4), (4), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(98), (28), (18), (6), (3), (3), (2), (2), (2), (2), (2), (1)
Host Rabbit
(44), (34), (20)
Clonality
Polyclonal
Conjugate This GTPase NRas antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(85), (56), (28), (23), (20), (19), (18), (5), (2), (1), (1), (1)
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Product Details anti-GTPase NRas Antibody

Target Details GTPase NRas Application Details Handling Images
Cross-Reactivity (Details) Expected species reactivity: Pig
Purification Purified
Immunogen A portion of amino acids 147-179 from the human protein was used as the immunogen for this NRAS antibody.
Isotype Ig Fraction

Target Details GTPase NRas

Product Details anti-GTPase NRas Antibody Application Details Handling Images back to top
Antigen
Alternative Name NRAS (NRAS Antibody Abstract)
Background NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).
UniProt P01111
Research Area Cancer
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Handling Images back to top
Application Notes Titration of the NRAS antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Restrictions For Research Use only

Handling

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Images back to top
Format Liquid
Buffer In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Aliquot the NRAS antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Images

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-GTPase NRas antibody (NRAS) (AA 147-179) (ABIN3031131) IHC analysis of FFPE human lung carcinoma tissue stained with NRAS antibody
Flow Cytometry (FACS) image for anti-GTPase NRas antibody (NRAS) (AA 147-179) (ABIN3031131) NRAS antibody flow cytometric analysis of NCI-H460 cells (green) compared to a negati...
Western Blotting (WB) image for anti-GTPase NRas antibody (NRAS) (AA 147-179) (ABIN3031131) NRAS antibody western blot analysis in MCF-7 lysate. Predicted molecular weight ~21 kDa.