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|Application / Reactivity||Human|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||10 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||7 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||2 Antibodies|
|Immunohistochemistry (IHC)||22 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||67 Antibodies|
|Antigen||GTPase NRas (NRAS) Antibodies|
|Conjugate||This GTPase NRas antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-GTPase NRas AntibodyTarget Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN360038) Images
|Specificity||This antibody reacts to NRAS.|
|Purification||Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human NRAS.|
Target Details GTPase NRasProduct Details anti-GTPase NRas Antibody Application Details Handling References for anti-GTPase NRas Antibody (ABIN360038) Images back to top
|Alternative Name||NRAS (NRAS Antibody Abstract)|
|Background||NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog|
|Gene ID||4893, 9606|
|Pathways||p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-GTPase NRas Antibody Target Details GTPase NRas Handling References for anti-GTPase NRas Antibody (ABIN360038) Images back to top
ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details References for anti-GTPase NRas Antibody (ABIN360038) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-GTPase NRas Antibody (ABIN360038)Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling Images back to top
Smalley, Contractor, Nguyen et al.: "Identification of a novel subgroup of melanomas with KIT/cyclin-dependent kinase-4 overexpression." in: Cancer research, Vol. 68, Issue 14, pp. 5743-52, 2008 (PubMed).
Banerji, Affolter, Judson et al.: "BRAF and NRAS mutations in melanoma: potential relationships to clinical response to HSP90 inhibitors." in: Molecular cancer therapeutics, Vol. 7, Issue 4, pp. 737-9, 2008 (PubMed).
ImagesProduct Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN360038) back to top