anti-Human GTPase NRas antibody for Immunohistochemistry

Recommended GTPase NRas Antibody (supplied by: Log in to see )

Antigen
GTPase NRas (NRAS) Antibodies
  • NRAS
  • AV095280
  • N-ras
  • ALPS4
  • NRAS1
  • NS6
  • N-RAS
  • fj99a10
  • wu:fj99a10
  • zgc:100761
  • zras-b1
  • H-RAS
  • HRAS
  • GTPase NRas
  • African clawed frog N-ras
  • neuroblastoma ras oncogene
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • Tsp_01117
  • n-ras
  • Nras
  • NRAS
  • nras
Epitope
Internal Region
23
9
7
7
6
5
5
4
2
2
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
101
27
18
6
3
3
2
2
2
2
2
1
Host
Goat
45
37
19
Clonality
Polyclonal
Conjugate
This GTPase NRas antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
88
58
29
23
21
19
18
5
2
1
1
1
Options
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Catalog No. ABIN302138
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.508871 ABIN409077 IHC (p) IHC ELISA WB Goat Log in to see Polyclonal 1
9.508871 ABIN2466026 IHC ELISA WB Goat Internal Region Log in to see Polyclonal
9.508871 ABIN4340652 IHC WB Mouse IgG1 Log in to see 5G7
9.508871 ABIN3031131 FACS IHC ELISA WB Rabbit Ig Fraction AA 147-179 Log in to see Polyclonal
9.508871 ABIN2435108 IHC ELISA Rabbit IgG Log in to see Polyclonal
1 ABIN4904578 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2564225 IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal
1 ABIN1584929 IP IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN2313463 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2433487 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2313469 IHC ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN2471745 IHC ELISA WB Mouse IgG2a kappa Log in to see AT2G9
1 ABIN2313420 IHC ELISA WB Mouse IgG2a kappa Log in to see
1 ABIN2671012 IHC WB Mouse IgG1 Log in to see OTI5G7
1 ABIN2313426 IHC ELISA WB Goat Log in to see Polyclonal
1 ABIN2769393 IHC (p) IHC ELISA WB Mouse IgG2a, kappa Log in to see AT2G9
1 ABIN2771527 IHC (p) IHC ELISA WB Goat Internal Region Log in to see Polyclonal
1 ABIN2793206 FACS IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
1 ABIN3059826 ICC FACS IF IHC WB Rabbit Log in to see Polyclonal

General

Antigen GTPase NRas (NRAS) Antibodies
Epitope Internal Region
(23), (9), (7), (7), (6), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(101), (27), (18), (6), (3), (3), (2), (2), (2), (2), (2), (1)
Host Goat
(45), (37), (19)
Clonality
Polyclonal
Conjugate This GTPase NRas antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Method Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(88), (58), (29), (23), (21), (19), (18), (5), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-GTPase NRas Antibody

Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas antibody (ABIN302138) Images
Blocking Peptide Blocking peptide for this product available: ABIN370329
Cross-Reactivity Mouse (Murine), Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-RMKKLNSSDDGTQ, from the internal region of the protein sequence according to NP_002515.1.

Target Details GTPase NRas

Product Details anti-GTPase NRas Antibody Application Details Handling References for anti-GTPase NRas antibody (ABIN302138) Images back to top
Antigen
Alternative Name NRAS (NRAS Antibody Abstract)
Background Synonyms: NRAS, neuroblastoma RAS viral (v-ras) oncogene homolog, N-ras, NRAS1, N-ras protein part 4, v-ras neuroblastoma RAS viral oncogene homolog
Gene ID 4893, 18176, 24605
NCBI Accession NP_002515
Research Area Cancer
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling

Application Details

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Handling References for anti-GTPase NRas antibody (ABIN302138) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 21 kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2 kDa according to NP_002515.1). In transfected HEK293 transiently expressing NRAS bands of approx. 21 kDa and 25 kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03 µg/mL.
IHC: In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration: 2-3 µg/mL.
Restrictions For Research Use only

Handling

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details References for anti-GTPase NRas antibody (ABIN302138) Images back to top
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

References for anti-GTPase NRas antibody (ABIN302138)

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling Images back to top
Background publications

Thomas, Edmiston, Alexander, Millikan, Groben, Hao, Tolbert, Berwick, Busam, Begg, Mattingly, Ollila, Tse, Hummer, Lee-Taylor, Conway: "Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 16, Issue 5, pp. 991-7, 2007

Images

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas antibody (ABIN302138) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-GTPase NRas (NRAS) (Internal Region) antibody (ABIN302138) EB08365 (2.5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antig...