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anti-Rat (Rattus) GTPase NRas antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended GTPase NRas Antibody (supplied by: Log in to see )

Antigen
GTPase NRas (NRAS) Antibodies
  • ALPS4
  • AV095280
  • fj99a10
  • H-RAS
  • HRAS
  • N-RAS
  • N-ras
  • NRAS
  • NRAS1
  • NS6
  • wu:fj99a10
  • zgc:100761
  • zras-b1
Epitope
Internal Region
21
10
9
8
4
3
3
3
2
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
93
19
14
3
1
Host
Goat
40
35
18
Clonality
Polyclonal
Conjugate
This GTPase NRas antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
87
60
30
26
21
21
18
5
2
1
1
Supplier
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Catalog No. ABIN375000
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089690165 ABIN372821 EIA IHC (p) WB Goat IgG Internal Region Log in to see Polyclonal
0.00089690165 ABIN738336 IF (p) IHC (p) WB Rabbit IgG AA 166-186 Log in to see Polyclonal
0.00089690165 ABIN409077 IHC (p) IHC ELISA WB Goat IgG Internal Region Log in to see Polyclonal
0.00089690165 ABIN1737523 IHC (p) ELISA WB Goat Internal Region Log in to see Polyclonal
0.00089690165 ABIN2963636 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal

Top referenced anti-GTPase NRas antibody for Immunohistochemistry (Paraffin-embedded Sections)

  • Dog (Canine) Polyclonal GTPase NRas Primary Antibody for IHC (p), WB - ABIN375000 (1 Publications): Thomas, Edmiston, Alexander, Millikan, Groben, Hao, Tolbert, Berwick, Busam, Begg, Mattingly, Ollila, Tse, Hummer, Lee-Taylor, Conway: Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma. in Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2007 (PubMed)

Similar anti-GTPase NRas Antibodies

Application / Reactivity Human Rat (Rattus) Dog (Canine) Mouse (Murine)
Western Blotting (WB) 90 Antibodies 17 Antibodies 4 Antibodies 13 Antibodies
Immunoprecipitation (IP) 2 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies 3 Antibodies 5 Antibodies 6 Antibodies
Immunohistochemistry (IHC) 23 Antibodies 9 Antibodies 7 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 27 Antibodies 1 Antibodies

General

Antigen GTPase NRas (NRAS) Antibodies
Epitope Internal Region
(21), (10), (9), (8), (4), (3), (3), (3), (2), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(93), (19), (14), (3), (1)
Host Goat
(40), (35), (18)
Clonality
Polyclonal
Conjugate This GTPase NRas antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(87), (60), (30), (26), (21), (21), (18), (5), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-GTPase NRas Antibody

Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN375000) Images
Sequence C-RMKKLNSSDD GTQ
Specificity This antibody detects NRAS.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
Immunogen Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS

Target Details GTPase NRas

Product Details anti-GTPase NRas Antibody Application Details Handling References for anti-GTPase NRas Antibody (ABIN375000) Images back to top
Antigen
Alternative Name NRAS (NRAS Antibody Abstract)
Background NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
Molecular Weight 21 kDa (NP_002515.1).
Gene ID 4893, 9606
UniProt P01111
Research Area Cancer
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Handling References for anti-GTPase NRas Antibody (ABIN375000) Images back to top
Application Notes Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details References for anti-GTPase NRas Antibody (ABIN375000) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-GTPase NRas Antibody (ABIN375000)

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling Images back to top
Background publications

Thomas, Edmiston, Alexander, Millikan, Groben, Hao, Tolbert, Berwick, Busam, Begg, Mattingly, Ollila, Tse, Hummer, Lee-Taylor, Conway: "Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma." in: Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, Vol. 16, Issue 5, pp. 991-7, 2007

Images

Product Details anti-GTPase NRas Antibody Target Details GTPase NRas Application Details Handling References for anti-GTPase NRas Antibody (ABIN375000) back to top
Supplier Images
 image for anti-GTPase NRas antibody (NRAS) (Internal Region) (ABIN375000) anti-GTPase NRas (NRAS) (Internal Region) antibody
 image for anti-GTPase NRas antibody (NRAS) (Internal Region) (ABIN375000) anti-GTPase NRas (NRAS) (Internal Region) antibody (Image 2)