anti-Dog (Canine) HMGB1 antibody for Flow Cytometry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • hmgb1
  • amphoterin
  • hmg-1
  • hmg1
  • hmg3
  • sbp-1
  • HMGB1
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • p30
  • Ac2-008
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • Hmgb1
  • high-mobility group box 1
  • high mobility group box 1
  • high mobility group protein B1-like
  • high-mobility group box 1a
  • HMG-box transcription factor 1
  • hmgb1
  • HMGB1
  • Hmgb1
  • LOC100392312
  • LOC100754755
  • LOC100359149
  • hmgb1a
  • Hbp1
Epitope
AA 100-200, Internal Region
69
61
38
38
31
19
19
19
19
16
15
15
12
12
11
11
8
6
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
450
231
160
59
41
36
22
13
13
10
10
10
7
5
3
2
1
Host
Rabbit
243
212
3
2
2
2
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
27
20
17
17
9
9
8
8
8
7
7
7
7
6
6
6
5
5
5
4
3
3
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
383
186
136
126
116
85
45
19
13
11
9
8
7
3
2
2
1
1
1
1
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Catalog No. ABIN250703
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4319384 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 100-200, Internal Region Log in to see Polyclonal 1
1 ABIN1735426 ICC FACS IF IHC WB Rabbit AA 100-200 Log in to see Polyclonal
1 ABIN407015 ELISA FACS ICC IF IHC IHC (p) WB DyLight 488 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319321 ELISA FACS ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319322 ELISA FACS ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319325 ELISA FACS ICC IF IHC IHC (p) FITC Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319326 FACS PE Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319327 ELISA FACS ICC IF IHC IHC (p) WB DyLight 405 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319383 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319385 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 100-200, Internal Region Log in to see Polyclonal
1 ABIN4319386 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 100-200, Internal Region Log in to see Polyclonal

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope AA 100-200, Internal Region
(69), (61), (38), (38), (31), (19), (19), (19), (19), (16), (15), (15), (12), (12), (11), (11), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(450), (231), (160), (59), (41), (36), (22), (13), (13), (10), (10), (10), (7), (5), (3), (2), (1)
Host Rabbit
(243), (212), (3), (2), (2), (2), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(27), (20), (17), (17), (9), (9), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(383), (186), (136), (126), (116), (85), (45), (19), (13), (11), (9), (8), (7), (3), (2), (2), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 antibody (ABIN250703) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 antibody (ABIN250703) Images back to top
Antigen
Alternative Name HMGB1/HMG-1 (HMGB1 Antibody Abstract)
Background Gene Symbol: HMGB1
Molecular Weight Theoretical MW: 29 kDa
Gene ID 3146
UniProt P09429
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 antibody (ABIN250703) Images back to top
Application Notes Western Blot 0.5-1.0 μg/mL, Simple Western 1:2000, Flow Cytometry 1:100, ELISA, Immunohistochemistry 1:100-1:250, Immunocytochemistry/Immunofluorescence 0.05 μg/mL, Immunohistochemistry-Paraffin 1:100-1:250This HMGB1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen approx. 29 kDa. It has been reported that the HRP conjugated format (Catalog# NB100-2322H) of this antibody works well for use in ELISA. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol WB Protocol specific for HMGB1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 35 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 2 hours at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-HMGB1 primary antibody in blocking buffer and incubate 3 hours at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 2 hours at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce's ECL).Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.IHC-P Protocol specific for HMGB1 Antibody Immunohistochemistry-paraffin embedded sectionsAntigen UnmaskingBring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. Staining
. Wash sections in dH2O three times for 5 minutes each.
. Wash section in wash buffer (1X PBS/0.1 % Tween-20 (1X PBST)) for 5 minutes.
. Block each section with 100-400 µL blocking solution (1X PBST, 5 % goat serum) for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL primary antibody diluted in 1X PBST, 5 % goat serum to each section. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated secondary antibody, diluted in 1X PBST, 5 % goat serum. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Striptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in dH2O.
. Counterstain sections in hematoxylin.
. Wash sections in dH2O two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 antibody (ABIN250703) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-HMGB1 antibody (ABIN250703)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Coleman, Zou, Crews: "Microglial-derived miRNA let-7 and HMGB1 contribute to ethanol-induced neurotoxicity via TLR7." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 22, 2017 Method employed by authors: Flow Cytometry (FACS), ELISA (ELISA) (Sample species: Human).

Kim, Lee, Oh, Park, Park, Hong, Kim, Lee, Ko, Choi, Hong, Jang: "Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model." in: Yonsei medical journal, Vol. 57, Issue 5, pp. 1095-105, 2016 (Sample species: Rabbit). Further details: Immunohistochemistry

Zhou, Wen, Cheng, Ma, Li, Ren, Sheng, Dong, Lu, Hu, Wang: "Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-κB signal pathway." in: Oncoimmunology, Vol. 5, Issue 7, pp. e1180485, 2016 Further details: Flow Cytometry

Feng, Yang, Ma, Bai, Chen: "Role of Toll-like receptors in diabetic renal lesions in a miniature pig model." in: Science advances, Vol. 1, Issue 5, pp. e1400183, 2015 (Sample species: Cow (Bovine)). Further details: Immunohistochemistry

Lu, Huang, Liao, Yen: "Ursolic acid triggers nonprogrammed death (necrosis) in human glioblastoma multiforme DBTRG-05MG cells through MPT pore opening and ATP decline." in: Molecular nutrition & food research, Vol. 58, Issue 11, pp. 2146-56, 2014

Zahedi, Barone, Wang, Murray-Stewart, Roy-Chaudhury, Smith, Casero, Soleimani: "Proximal tubule epithelial cell specific ablation of the spermidine/spermine N1-acetyltransferase gene reduces the severity of renal ischemia/reperfusion injury." in: PLoS ONE, Vol. 9, Issue 11, pp. e110161, 2014 (Sample species: Human). Further details: Western Blotting

Kang, Tang, Schapiro, Loux, Livesey, Billiar, Wang, Van Houten, Lotze, Zeh: "The HMGB1/RAGE inflammatory pathway promotes pancreatic tumor growth by regulating mitochondrial bioenergetics." in: Oncogene, Vol. 33, Issue 5, pp. 567-77, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Robinson, Caldis, Harp, Goings, Miller: "High-mobility group box 1 protein (HMGB1) neutralization ameliorates experimental autoimmune encephalomyelitis." in: Journal of autoimmunity, Vol. 43, pp. 32-43, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Jiang, Scott, Kwak, Chen, Mardon: "Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling." in: Oncogene, Vol. 30, Issue 29, pp. 3248-60, 2011

Montie, Pestell, Merry: "SIRT1 modulates aggregation and toxicity through deacetylation of the androgen receptor in cell models of SBMA." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 48, pp. 17425-36, 2011

Fazio, Dimattia, Chadi, Kernohan, Pin: "Stanniocalcin 2 alters PERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice." in: BMC cell biology, Vol. 12, pp. 17, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Tang, Kang, Livesey, Kroemer, Billiar, Van Houten, Zeh, Lotze: "High-mobility group box 1 is essential for mitochondrial quality control." in: Cell metabolism, Vol. 13, Issue 6, pp. 701-11, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Barlan, Griffin, McGuire, Wiethoff: "Adenovirus membrane penetration activates the NLRP3 inflammasome." in: Journal of virology, Vol. 85, Issue 1, pp. 146-55, 2010 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 antibody (ABIN250703) back to top
Supplier Images
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Detection of HMGB1 in whole cell HeLa lysate using ABIN250703. ECL exposure, 1 min.
Immunohistochemistry (IHC) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Staining of HMGB1 in mouse liver using ABIN250703.
Simple Western (SimWes) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Simple Western: HMGB1/HMG-1 Antibody [ABIN250703] - Simple Western lane view shows a ...
ELISA image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) ELISA: HMGB1/HMG-1 Antibody [ABIN250703] - A dose-dependent titration of the HRP conj...
Flow Cytometry (FACS) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Flow Cytometry: HMGB1/HMG-1 Antibody [ABIN250703] - Staining of NTERA-2 cells using A...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Western Blot: HMGB1/HMG-1 Antibody [ABIN250703] - Total protein from SHSY-5Y, MCF7, N...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Western Blot: HMGB1/HMG-1 Antibody [ABIN250703] - Hepatocyte protein lysate at 1:1000...
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Western Blot: HMGB1/HMG-1 Antibody [ABIN250703] - HMGB1 antibody was tested in HeLa c...
Immunofluorescence (IF) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [ABIN250703] - HeLa cell...
Immunofluorescence (IF) image for anti-High-Mobility Group Box 1 (HMGB1) (AA 100-200), (Internal Region) antibody (ABIN250703) Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody - Neuro2a cells were fi...