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anti-Horse (Equine) HMGB1 antibody for Immunocytochemistry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Epitope
AA 100-200
65
52
39
39
36
19
19
18
17
16
13
12
12
11
11
8
6
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
397
184
115
30
26
22
10
9
6
6
3
1
1
Host
Rabbit
229
179
4
4
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
24
17
16
14
8
7
7
6
6
6
6
6
5
5
4
4
4
4
4
4
3
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
345
178
129
87
83
62
23
12
11
11
6
6
2
1
1
1
1
Supplier
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Catalog No. ABIN250705
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Similar anti-HMGB1 Antibodies

Application / Reactivity Human Pig (Porcine) Dog (Canine) Cow (Bovine) Mouse (Murine) Rat (Rattus) Horse (Equine) Rabbit Sheep (Ovine)
Western Blotting (WB) 338 Antibodies 20 Antibodies 25 Antibodies 30 Antibodies 156 Antibodies 88 Antibodies 1 Antibodies 9 Antibodies 10 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 12 Antibodies 1 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope AA 100-200
(65), (52), (39), (39), (36), (19), (19), (18), (17), (16), (13), (12), (12), (11), (11), (8), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
(397), (184), (115), (30), (26), (22), (10), (9), (6), (6), (3), (1), (1)
Host Rabbit
(229), (179), (4), (4), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(24), (17), (16), (14), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(345), (178), (129), (87), (83), (62), (23), (12), (11), (11), (6), (6), (2), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN250705) Images
Cross-Reactivity (Details) Predicted to react with porcine, equine,rabbit based on 100% sequence homology.
Purification affinity purified
Immunogen A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200).

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN250705) Images back to top
Antigen
Alternative Name HMGB1/HMG-1 (HMGB1 Antibody Abstract)
Background HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins1 and 2. These proteins (containing multiple HMG-boxes) are conserved domains of 80amino acids which mediate the DNA binding of many proteins. HMG box domainsrecognize DNA structure. Both HMGB1 and HMGB2 contain an N-terminal HMG box, acentral HMG box, and an acidic carboxy terminus. The acidic tails of these proteinscontain multiple serine residues which match the phosphorylation consensus sites ofcasein kinase II, and phosphorylation of this domain appears to be important for properfunctioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate thebinding of various sequence-specific transcription factors to their respective DNAbinding sites. They may also serve as architectural factors that recognize and mediateDNA structural changes that accompany various events such as DNA repair,transcription, and replication. Alternate Names: anti-Amphoterin antibody, anti-High mobility group 1 antibody, anti-High Mobility GroupBox 1 antibody, anti-High mobility group protein 1 antibody, anti-HMG3 antibody,anti-Hmgb1 protein antibody, anti-Nonhistone chromosomal protein HMG1 antibody,anti-SBP-1 antibody, anti-Sulfoglucuronyl carbohydrate binding protein antibody.
Gene Symbol: HMGB1
Gene ID 3146, 15289, 25459
UniProt P09429
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN250705) Images back to top
Application Notes This HMGB1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen ~29 kDa.
Recommended dilutions: Flow Cytometry 1:100, Immunocytochemistry/Immunofluorescence 0.05 µg/mL, Immunohistochemistry 1:100-1:250, Immunohistochemistry-Paraffin 1:100-1:250, Western Blot 0.5-1.0 µg/mL
Protocol WB Protocol specific for HMGB1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 35 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 2 hours at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-HMGB1 primary antibody in blocking buffer and incubate 3 hours at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 2 hours at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce's ECL).Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.IHC-P Protocol specific for HMGB1 Antibody Immunohistochemistry-paraffin embedded sectionsAntigen UnmaskingBring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. Staining
. Wash sections in dH2O three times for 5 minutes each.
. Wash section in wash buffer (1X PBS/0.1 % Tween-20 (1X PBST)) for 5 minutes.
. Block each section with 100-400 µL blocking solution (1X PBST, 5 % goat serum) for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL primary antibody diluted in 1X PBST, 5 % goat serum to each section. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated secondary antibody, diluted in 1X PBST, 5 % goat serum. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Striptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in dH2O.
. Counterstain sections in hematoxylin.
. Wash sections in dH2O two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN250705) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C

References for anti-HMGB1 Antibody (ABIN250705)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Kim, Lee, Oh, Park, Park, Hong, Kim, Lee, Ko, Choi, Hong, Jang: "Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model." in: Yonsei medical journal, Vol. 57, Issue 5, pp. 1095-105, 2016 (Sample species: Rabbit). Further details: Immunohistochemistry

Zhou, Wen, Cheng, Ma, Li, Ren, Sheng, Dong, Lu, Hu, Wang: "Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-κB signal pathway." in: Oncoimmunology, Vol. 5, Issue 7, pp. e1180485, 2016 Further details: Flow Cytometry

Feng, Yang, Ma, Bai, Chen: "Role of Toll-like receptors in diabetic renal lesions in a miniature pig model." in: Science advances, Vol. 1, Issue 5, pp. e1400183, 2015 (Sample species: Cow (Bovine)). Further details: Immunohistochemistry

Lu, Huang, Liao, Yen: "Ursolic acid triggers nonprogrammed death (necrosis) in human glioblastoma multiforme DBTRG-05MG cells through MPT pore opening and ATP decline." in: Molecular nutrition & food research, Vol. 58, Issue 11, pp. 2146-56, 2014

Zahedi, Barone, Wang, Murray-Stewart, Roy-Chaudhury, Smith, Casero, Soleimani: "Proximal tubule epithelial cell specific ablation of the spermidine/spermine N1-acetyltransferase gene reduces the severity of renal ischemia/reperfusion injury." in: PLoS ONE, Vol. 9, Issue 11, pp. e110161, 2014 (Sample species: Human). Further details: Western Blotting

Kang, Tang, Schapiro, Loux, Livesey, Billiar, Wang, Van Houten, Lotze, Zeh: "The HMGB1/RAGE inflammatory pathway promotes pancreatic tumor growth by regulating mitochondrial bioenergetics." in: Oncogene, Vol. 33, Issue 5, pp. 567-77, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Robinson, Caldis, Harp, Goings, Miller: "High-mobility group box 1 protein (HMGB1) neutralization ameliorates experimental autoimmune encephalomyelitis." in: Journal of autoimmunity, Vol. 43, pp. 32-43, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Jiang, Scott, Kwak, Chen, Mardon: "Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling." in: Oncogene, Vol. 30, Issue 29, pp. 3248-60, 2011

Montie, Pestell, Merry: "SIRT1 modulates aggregation and toxicity through deacetylation of the androgen receptor in cell models of SBMA." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 48, pp. 17425-36, 2011

Fazio, Dimattia, Chadi, Kernohan, Pin: "Stanniocalcin 2 alters PERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice." in: BMC cell biology, Vol. 12, pp. 17, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Tang, Kang, Livesey, Kroemer, Billiar, Van Houten, Zeh, Lotze: "High-mobility group box 1 is essential for mitochondrial quality control." in: Cell metabolism, Vol. 13, Issue 6, pp. 701-11, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Barlan, Griffin, McGuire, Wiethoff: "Adenovirus membrane penetration activates the NLRP3 inflammasome." in: Journal of virology, Vol. 85, Issue 1, pp. 146-55, 2010 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN250705) back to top
Supplier Images
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250705) Detection of HMGB1 in whole cell HeLa lysate using ABIN250703. ECL exposure, 1 min.
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250705) Staining of HMGB1 in mouse liver using ABIN250703.