anti-Human HMGB1 antibody for Flow Cytometry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • hmgb1
  • amphoterin
  • hmg-1
  • hmg1
  • hmg3
  • sbp-1
  • HMGB1
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • p30
  • Ac2-008
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • Hmgb1
  • high-mobility group box 1
  • high mobility group box 1
  • high mobility group protein B1-like
  • high-mobility group box 1a
  • HMG-box transcription factor 1
  • hmgb1
  • HMGB1
  • Hmgb1
  • LOC100392312
  • LOC100754755
  • LOC100359149
  • hmgb1a
  • Hbp1
Epitope
AA 2-215
69
60
38
38
32
20
19
19
16
15
15
12
12
12
11
11
8
6
5
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
443
232
161
60
41
37
23
14
13
10
10
10
7
5
3
2
1
Host
Mouse
243
205
3
2
2
2
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
27
20
17
17
9
9
8
8
8
7
7
7
7
6
6
6
5
5
5
4
3
3
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
377
186
132
127
113
85
45
18
13
12
9
8
5
3
2
2
1
1
1
1
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Catalog No. ABIN4899429
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.326658 ABIN4899428 FACS ICC IP WB Mouse IgG2b AA 2-215 Log in to see 115603 13
14.326658 ABIN250703 ELISA FACS ICC IF IHC IHC (p) SimWes WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 13
14.326658 ABIN4896096 FACS Allophycocyanin Mouse IgG2b AA 2-215 Log in to see 115603
14.326658 ABIN4896098 FACS PE Mouse IgG2b AA 2-215 Log in to see 115603
14.326658 ABIN4896097 FACS Alexa Fluor 488 Mouse IgG2b AA 2-215 Log in to see 115603
14.326658 ABIN2662753 FACS PE Mouse IgG2b kappa Log in to see 3E8 7
14.326658 ABIN4319335 CyTOF FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3
14.326658 ABIN4319342 CyTOF FACS IHC IHC (p) SimWes WB Mouse IgG2b kappa A Box Domain, AA 9-79, full length Log in to see 19N10B7
14.326658 ABIN4319346 FACS IHC (p) WB Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
14.326658 ABIN4319340 CyTOF FACS IHC IHC (p) SimWes WB Mouse IgG2b kappa A Box Domain, AA 9-79, full length Log in to see 19N10B7
14.326658 ABIN4319337 FACS IHC (p) SimWes WB Rabbit Log in to see Polyclonal
1 ABIN2474000 FACS IF WB Hamster IgG Log in to see HMG1-5H6 1
1 ABIN2656846 FACS Alexa Fluor 594 Mouse IgG2b kappa Log in to see 3E8 7
1 ABIN5027246 FACS IHC WB Mouse IgG1 kappa AA 1-200 Log in to see ABM24D3
1 ABIN4319384 ELISA FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 100-200, Internal Region Log in to see Polyclonal 1
1 ABIN2736903 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1498682 FACS IHC (p) WB Mouse kappa, IgG1 Log in to see IMG19N15F4
1 ABIN1498681 FACS IHC (p) WB Mouse IgG2b, kappa Log in to see IMG19N10B7
1 ABIN1735426 ICC FACS IF IHC WB Rabbit AA 100-200 Log in to see Polyclonal
1 ABIN1102112 FACS IHC (p) WB Mouse IgG2b, kappa Log in to see IMG19N10B7

Top referenced anti-HMGB1 antibody for Flow Cytometry

Similar anti-HMGB1 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 5 Antibodies
ELISA 176 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 113 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 43 Antibodies
Immunofluorescence (IF) 82 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 119 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 131 Antibodies
Immunoprecipitation (IP) 17 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Simple Western (SimWes) 12 Antibodies
Western Blotting (WB) 362 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope AA 2-215
(69), (60), (38), (38), (32), (20), (19), (19), (16), (15), (15), (12), (12), (12), (11), (11), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(443), (232), (161), (60), (41), (37), (23), (14), (13), (10), (10), (10), (7), (5), (3), (2), (1)
Host Mouse
(243), (205), (3), (2), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(27), (20), (17), (17), (9), (9), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(377), (186), (132), (127), (113), (85), (45), (18), (13), (12), (9), (8), (5), (3), (2), (2), (1), (1), (1), (1)
Pubmed 13 references available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling ProductDetails: References for anti-HMGB1 antibody (ABIN4899429) Images
Specificity Detects human HMGB1/HMG-1 in direct ELISAs and Western blots.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen E. coli-derived recombinant human HMGB1/HMG-1, Gly2-Glu215 (predicted), Accession # P09429
Clone 115603
Isotype IgG2b

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling ProductDetails: References for anti-HMGB1 antibody (ABIN4899429) Images back to top
Antigen
Alternative Name HMGB1/HMG-1 (HMGB1 Antibody Abstract)
Background Alternative Names: Amphoterin, high mobility group box 1, High mobility group protein 1, high mobility group protein B1, high-mobility group (nonhistone chromosomal) protein 1, high-mobility group box 1, HMG1, HMG-1, HMG1DKFZp686A04236, HMG3, SBP-1, Sulfoglucuronyl carbohydrate binding protein
Gene ID 3146
UniProt P09429
Research Area Neurology, Chromatin Binding Proteins, Transcription Factors, Chromatin and Nuclear Signaling
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling ProductDetails: References for anti-HMGB1 antibody (ABIN4899429) Images back to top
Application Notes Western Blot 0.05 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Chromatin Immunoprecipitation (ChIP) 5 μg/5 x 10^6 cells
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details ProductDetails: References for anti-HMGB1 antibody (ABIN4899429) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

ProductDetails: References for anti-HMGB1 antibody (ABIN4899429)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Haruma, Teshigawara, Hishikawa, Wang, Liu, Wake, Mori, Takahashi, Sugiu, Date, Nishibori: "Anti-high mobility group box-1 (HMGB1) antibody attenuates delayed cerebral vasospasm and brain injury after subarachnoid hemorrhage in rats." in: Scientific reports, Vol. 6, pp. 37755, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)).

Moser, Janik, Schiefer, Müllauer, Bekos, Scharrer, Mildner, Rényi-Vámos, Klepetko, Ankersmit: "Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology." in: PLoS ONE, Vol. 9, Issue 4, pp. e94118, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Donnelly, Errington-Mais, Steele, Hadac, Jennings, Scott, Peach, Phillips, Bond, Pandha, Harrington, Vile, Russell, Selby, Melcher: "Measles virus causes immunogenic cell death in human melanoma." in: Gene therapy, Vol. 20, Issue 1, pp. 7-15, 2013 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Liou, Adler, Keogh, Li, Jensen, Walsh, Packer, Clark, Carveth, Chen, Rogers, Lane, Moore, Sturrock, Paine, Cox, Hoidal: "Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis." in: PLoS ONE, Vol. 7, Issue 8, pp. e42748, 2012 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Sperling, O?dak, Walch-Rückheim, Wickenhauser, Doorbar, Pfister, Malejczyk, Majewski, Keates, Smola: "Human papillomavirus type 8 interferes with a novel C/EBP?-mediated mechanism of keratinocyte CCL20 chemokine expression and Langerhans cell migration." in: PLoS pathogens, Vol. 8, Issue 7, pp. e1002833, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Vitali, Stronati, Negroni, Di Nardo, Pierdomenico, del Giudice, Rossi, Cucchiara: "Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease." in: The American journal of gastroenterology, Vol. 106, Issue 11, pp. 2029-40, 2011 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Davé, Tilstra, Matsuoka, Li, DeMarco, Beer-Stolz, Sepulveda, Fink, Lotze, Plevy: "Ethyl pyruvate decreases HMGB1 release and ameliorates murine colitis." in: Journal of leukocyte biology, Vol. 86, Issue 3, pp. 633-43, 2009 Method employed by authors: ELISA (ELISA) (Sample species: Mouse (Murine)).

Zhou, Huang, Poon, Chen, Chan, Ng, Guan, Watt, Lu, Yuen, Zheng: "Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection." in: Journal of hepatology, Vol. 51, Issue 2, pp. 322-32, 2009 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Aychek, Miller, Sagi-Assif, Levy-Nissenbaum, Israeli-Amit, Pasmanik-Chor, Jacob-Hirsch, Amariglio, Rechavi, Witz: "E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release." in: International journal of cancer, Vol. 123, Issue 8, pp. 1741-50, 2008 Method employed by authors: Immunodepletion (IDe) (Sample species: Human).

Rowe, Jackson, Liu, Hardison, Livraghi, Solomon, McQuaid, Noerager, Gaggar, Clancy, ONeal, Sorscher, Abraham, Blalock: "Potential role of high-mobility group box 1 in cystic fibrosis airway disease." in: American journal of respiratory and critical care medicine, Vol. 178, Issue 8, pp. 822-31, 2008 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Zetterström, Jiang, Wähämaa, Ostberg, Aveberger, Schierbeck, Lotze, Andersson, Pisetsky, Erlandsson Harris: "Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalate." in: Journal of leukocyte biology, Vol. 83, Issue 1, pp. 31-8, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Rao, Tracey, Pober: "IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 10, pp. 6536-46, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Seki, De Minicis, Osterreicher, Kluwe, Osawa, Brenner, Schwabe: "TLR4 enhances TGF-beta signaling and hepatic fibrosis." in: Nature medicine, Vol. 13, Issue 11, pp. 1324-32, 2007 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling ProductDetails: References for anti-HMGB1 antibody (ABIN4899429) back to top
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 2-215) (ABIN4899429) Detection of HMGB1/HMG-1-regulated Genes by Chromatin Immunoprecipitation. HepG2 huma...
Flow Cytometry (FACS) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 2-215) (ABIN4899429) Detection of HMGB1 in HCT-116 Human Cell Line by Flow Cytometry. HCT-116 human colore...
Immunocytochemistry (ICC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 2-215) (ABIN4899429) HMGB1/HMG-1 in CCD-18Co Human Cell Line. HMGB1/HMG-1 was detected in immersion fixed ...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 2-215) (ABIN4899429) Detection of Human HMGB1/HMG-1 by Western Blot. Western blot shows lysates of HepG2 h...