You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human HMGB1 antibody for Flow Cytometry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Reactivity
Human, Mouse (Murine), Rat (Rattus)
396
183
114
30
26
22
10
9
6
6
3
1
1
Host
Mouse
229
178
4
4
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is conjugated to PE
24
17
16
13
8
7
7
6
6
6
6
6
5
5
4
4
4
4
4
4
3
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
345
178
129
87
82
62
23
12
11
11
6
6
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2662753
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010463598 ABIN2656846 FACS Alexa Fluor 594 Mouse IgG2b kappa Log in to see 3E8 7
0.0010463598 ABIN250705 ICC FACS IF IHC (p) IHC WB Rabbit AA 100-200 Log in to see Polyclonal
0.0010463598 ABIN1498682 FACS IHC (p) WB Mouse kappa, IgG1 Log in to see IMG19N15F4
0.0010463598 ABIN1498681 FACS IHC (p) WB Mouse IgG2b, kappa Log in to see IMG19N10B7
0.0010463598 ABIN1102112 FACS IHC (p) WB Mouse IgG2b, kappa Log in to see IMG19N10B7
0.0010463598 ABIN2474000 FACS IF WB Hamster IgG Log in to see HMG1-5H6 1
0.0010463598 ABIN1735426 ICC FACS IF IHC WB Rabbit Internal Region, AA 100-200 Log in to see Polyclonal
0.0010463598 ABIN1902518 FACS ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term, AA 16-44 Log in to see Polyclonal
0.0010463598 ABIN1902519 FACS ELISA WB APC Rabbit IgG N-Term, AA 16-44 Log in to see Polyclonal
0.0010463598 ABIN1902520 FACS ELISA WB PE Rabbit IgG N-Term, AA 16-44 Log in to see Polyclonal
0.0010463598 ABIN2662754 FACS PE Mouse IgG2b kappa Log in to see 3E8 7
0.0010463598 ABIN448273 ICC FACS IF IHC (p) IHC WB Rabbit IgG Log in to see EPR3507
0.0010463598 ABIN448285 FACS IHC (p) IHC WB Rabbit IgG Log in to see EPR3506
0.0010463598 ABIN2283389 FACS APC Mouse IgG2b AA 2-215 Log in to see 115603
0.0010463598 ABIN2736903 FACS IHC WB Rabbit IgG Log in to see Polyclonal
0.0010463598 ABIN1724246 FACS IHC (p) WB Mouse IgG2b kappa Log in to see IMG19N10B7
0.0010463598 ABIN1724247 FACS IHC (p) WB Mouse IgG1 kappa Log in to see IMG19N15F4
0.0010463598 ABIN960204 FACS IHC (p) WB Rabbit AA 100-150 Log in to see Polyclonal
0.0010463598 ABIN1839107 FACS WB Rabbit N-Term, AA 9-36 Log in to see Polyclonal
0.0010463598 ABIN4319328 FACS Alexa Fluor 700 Mouse IgG2b AA 2-215 Log in to see 115603

Top referenced anti-HMGB1 antibody for Flow Cytometry

Similar anti-HMGB1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 338 Antibodies 156 Antibodies 88 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 12 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 131 Antibodies 70 Antibodies 27 Antibodies
Immunohistochemistry (IHC) 89 Antibodies 40 Antibodies 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(396), (183), (114), (30), (26), (22), (10), (9), (6), (6), (3), (1), (1)
Host Mouse
(229), (178), (4), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is conjugated to PE
(24), (17), (16), (13), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS)
(345), (178), (129), (87), (82), (62), (23), (12), (11), (11), (6), (6), (2), (1), (1), (1), (1)
Pubmed 7 references available
Supplier Log in to see

Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2662753) Images
Purification The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Clone 3E8
Isotype IgG2b kappa

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN2662753) Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is expressed as a 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Human HMGB1 is 100 % aa identical to canine HMGB1 and 99 % aa identical to mouse, rat, bovine and porcine HMGB1. HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 also plays a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis. It acts as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN2662753) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN2662753) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-HMGB1 Antibody (ABIN2662753)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Background publications

Zhou, Wang, Wang, Jia, Li, Wang, Wu, Tang: "Generation of monoclonal antibodies against highly conserved antigens." in: PLoS ONE, Vol. 4, Issue 6, pp. e6087, 2009

Campana, Bosurgi, Rovere-Querini: "HMGB1: a two-headed signal regulating tumor progression and immunity." in: Current opinion in immunology, Vol. 20, Issue 5, pp. 518-23, 2008

Klune, Dhupar, Cardinal, Billiar, Tsung: "HMGB1: endogenous danger signaling." in: Molecular medicine (Cambridge, Mass.), Vol. 14, Issue 7-8, pp. 476-84, 2008

Dumitriu, Baruah, Manfredi, Bianchi, Rovere-Querini: "HMGB1: guiding immunity from within." in: Trends in immunology, Vol. 26, Issue 7, pp. 381-7, 2005

Müller, Ronfani, Bianchi: "Regulated expression and subcellular localization of HMGB1, a chromatin protein with a cytokine function." in: Journal of internal medicine, Vol. 255, Issue 3, pp. 332-43, 2004

Bonaldi, Talamo, Scaffidi, Ferrera, Porto, Bachi, Rubartelli, Agresti, Bianchi: "Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion." in: The EMBO journal, Vol. 22, Issue 20, pp. 5551-60, 2003

Thomas, Travers: "HMG1 and 2, and related 'architectural' DNA-binding proteins." in: Trends in biochemical sciences, Vol. 26, Issue 3, pp. 167-74, 2001

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2662753) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-HMGB1 antibody (High-Mobility Group Box 1)  (PE) (ABIN2662753) anti-High-Mobility Group Box 1 (HMGB1) antibody (PE)