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anti-Human HMGB1 antibody for Intracellular Flow Cytometry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Reactivity
Human, Mouse (Murine), Rat (Rattus)
295
112
101
19
19
17
6
6
3
1
1
1
Host
Mouse
213
93
4
4
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
20
15
11
9
8
7
2
1
1
1
1
1
1
1
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
251
158
75
60
53
27
14
12
11
6
6
1
1
1
1
1
Supplier
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Catalog No. ABIN2666344
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008704084 ABIN2666345 ICFC Mouse IgG2b kappa Log in to see 3E8 7

Top referenced anti-HMGB1 antibody for Intracellular Flow Cytometry

Similar anti-HMGB1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 244 Antibodies 86 Antibodies 76 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 12 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 62 Antibodies 22 Antibodies 18 Antibodies
Immunohistochemistry (IHC) 77 Antibodies 28 Antibodies 27 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(295), (112), (101), (19), (19), (17), (6), (6), (3), (1), (1), (1)
Host Mouse
(213), (93), (4), (4), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(20), (15), (11), (9), (8), (7), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(251), (158), (75), (60), (53), (27), (14), (12), (11), (6), (6), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2666344) Images
Purification The antibody was purified by affinity chromatography.
Clone 3E8
Isotype IgG2b kappa

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN2666344) Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is expressed as a 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Human HMGB1 is 100 % aa identical to canine HMGB1 and 99 % aa identical to mouse, rat, bovine and porcine HMGB1. HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 also plays a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis. It acts as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN2666344) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN2666344) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Upon receipt, store undiluted between 2°C and 8°C.

References for anti-HMGB1 Antibody (ABIN2666344)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Background publications

Zhou, Wang, Wang, Jia, Li, Wang, Wu, Tang: "Generation of monoclonal antibodies against highly conserved antigens." in: PLoS ONE, Vol. 4, Issue 6, pp. e6087, 2009

Campana, Bosurgi, Rovere-Querini: "HMGB1: a two-headed signal regulating tumor progression and immunity." in: Current opinion in immunology, Vol. 20, Issue 5, pp. 518-23, 2008

Klune, Dhupar, Cardinal, Billiar, Tsung: "HMGB1: endogenous danger signaling." in: Molecular medicine (Cambridge, Mass.), Vol. 14, Issue 7-8, pp. 476-84, 2008

Dumitriu, Baruah, Manfredi, Bianchi, Rovere-Querini: "HMGB1: guiding immunity from within." in: Trends in immunology, Vol. 26, Issue 7, pp. 381-7, 2005

Müller, Ronfani, Bianchi: "Regulated expression and subcellular localization of HMGB1, a chromatin protein with a cytokine function." in: Journal of internal medicine, Vol. 255, Issue 3, pp. 332-43, 2004

Bonaldi, Talamo, Scaffidi, Ferrera, Porto, Bachi, Rubartelli, Agresti, Bianchi: "Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion." in: The EMBO journal, Vol. 22, Issue 20, pp. 5551-60, 2003

Thomas, Travers: "HMG1 and 2, and related 'architectural' DNA-binding proteins." in: Trends in biochemical sciences, Vol. 26, Issue 3, pp. 167-74, 2001

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2666344) back to top
Supplier Images
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN2666344) anti-High-Mobility Group Box 1 (HMGB1) antibody
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN2666344) anti-High-Mobility Group Box 1 (HMGB1) antibody (Image 2)