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anti-Human HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • Ac2-008
  • amphoterin
  • DEF
  • hmg-1
  • HMG-1
  • hmg1
  • HMG1
  • Hmg1
  • HMG3
  • hmg3
  • Hmgb1
  • HMGB1
  • hmgb1
  • ik:tdsubc_1a5
  • p30
  • sbp-1
  • SBP-1
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
Epitope
N-Term
65
52
39
39
36
19
19
18
16
16
13
12
12
11
11
8
6
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
396
183
114
29
25
21
10
9
6
5
3
1
Host
Rabbit
228
179
4
4
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
24
17
16
14
8
7
7
6
6
6
6
6
5
5
4
4
4
4
4
4
3
2
1
1
1
1
1
1
Application
Western Blotting (WB)
344
178
129
87
83
62
23
12
11
11
6
6
2
1
1
1
1
Supplier
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Catalog No. ABIN2780399
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12650052 ABIN1449284 WB Mouse IgG Internal Region Log in to see 4C9 3
0.12650052 ABIN2780400 WB Rabbit Middle Region Log in to see Polyclonal 1
0.12650052 ABIN2776383 WB Rabbit Middle Region Log in to see Polyclonal 1
0.12650052 ABIN1107584 WB Mouse IgG2a Log in to see KS1 1
0.12650052 ABIN1533598 ELISA WB Rabbit IgG Log in to see Polyclonal 2
0.12650052 ABIN671616 IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 5
0.12650052 ABIN2787911 WB Rabbit N-Term Log in to see Polyclonal
0.12650052 ABIN2787912 WB Rabbit Middle Region Log in to see Polyclonal
0.12650052 ABIN356226 EIA IHC (p) WB Mouse IgG2b AA 1-215, N-Term Log in to see J2E1
0.12650052 ABIN561286 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 1-90, partial Log in to see 2F6 2
0.12650052 ABIN1326935 IF IHC (p) ELISA WB Mouse IgG1 kappa AA 1-215, full length Log in to see 1E6-E10 2
0.12650052 ABIN561282 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 1-215, full length Log in to see 1B11 2
0.12650052 ABIN561281 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 1-215, full length Log in to see 1D5 5
0.12650052 ABIN1536601 IHC (p) WB Mouse IgM AA 152-180, C-Term Log in to see 393CT23-1-4
0.12650052 ABIN614615 EIA IF IHC (p) WB Mouse IgG2a AA 1-216 Log in to see 1D5
0.12650052 ABIN1536741 WB Rabbit Ig AA 152-180, C-Term Log in to see Polyclonal
0.12650052 ABIN2854997 ICC IF IHC (p) IHC WB Rabbit IgG Center Log in to see Polyclonal
0.12650052 ABIN658396 WB Rabbit Ig Log in to see Polyclonal
0.12650052 ABIN250705 ICC FACS IF IHC (p) IHC WB Rabbit AA 100-200 Log in to see Polyclonal
0.12650052 ABIN395610 IF WB Mouse IgG2a kappa AA 1-91 Log in to see 2F6

Top referenced anti-HMGB1 antibody for Western Blotting

Similar anti-HMGB1 Antibodies

Application / Reactivity Human Pig (Porcine) Dog (Canine) Cow (Bovine) Mouse (Murine) Rat (Rattus) Chicken Xenopus laevis
Western Blotting (WB) 338 Antibodies 20 Antibodies 25 Antibodies 30 Antibodies 156 Antibodies 88 Antibodies 4 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunoprecipitation (IP) 12 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies 131 Antibodies 18 Antibodies 70 Antibodies 6 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope N-Term
(65), (52), (39), (39), (36), (19), (19), (18), (16), (16), (13), (12), (12), (11), (11), (8), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(396), (183), (114), (29), (25), (21), (10), (9), (6), (5), (3), (1)
Host Rabbit
(228), (179), (4), (4), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(24), (17), (16), (14), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(344), (178), (129), (87), (83), (62), (23), (12), (11), (11), (6), (6), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) Images
Blocking Peptide Blocking peptide for this product available: ABIN978051
Sequence GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against HMGB1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HMGB1

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background HMGB1 belongs to the HMGB family. It contains 2 HMG box DNA-binding domains. The protein binds preferentially single-stranded DNA and unwinds double stranded DNA. The oxidized HMGB1 may accumulate even in cells under oxidative stress.
Molecular Weight 25 kDa
Gene ID 3146
NCBI Accession NP_002119, NM_002128
UniProt P09429
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 215 AA

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN2780399) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGB1 Antibody (ABIN2780399)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Straino, Di Carlo, Mangoni, De Mori, Guerra, Maurelli, Panacchia, Di Giacomo, Palumbo, Di Campli, Uccioli, Biglioli, Bianchi, Capogrossi, Germani: "High-mobility group box 1 protein in human and murine skin: involvement in wound healing." in: The Journal of investigative dermatology, Vol. 128, Issue 6, pp. 1545-53, 2008

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2780399) back to top
Supplier Images
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (N-Term) (ABIN2780399) WB Suggested Anti-HMGB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: