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anti-Mouse (Murine) HMGB1 antibody for Flow Cytometry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • hmgb1
  • amphoterin
  • hmg-1
  • hmg1
  • hmg3
  • sbp-1
  • HMGB1
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • p30
  • Ac2-008
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • Hmgb1
  • high-mobility group box 1
  • high mobility group box 1
  • high mobility group protein B1-like
  • high-mobility group box 1a
  • HMG-box transcription factor 1
  • hmgb1
  • HMGB1
  • Hmgb1
  • LOC100392312
  • LOC100754755
  • LOC100359149
  • hmgb1a
  • Hbp1
Epitope
AA 100-200, Internal Region
67
53
37
37
37
19
18
18
18
17
13
13
12
12
11
11
7
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
425
204
138
50
39
29
13
12
6
6
3
1
1
Host
Rabbit
235
199
4
4
2
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
27
18
18
16
8
8
8
7
7
7
7
7
7
6
6
5
5
5
5
3
2
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
358
192
130
120
101
82
44
15
11
11
6
6
5
2
1
1
1
1
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Catalog No. ABIN250703
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.099335 ABIN2662753 FACS PE Mouse IgG2b kappa Log in to see 3E8 7
13.099335 ABIN4319342 CyTOF FACS IHC IHC (p) SimWes WB Mouse IgG2b kappa A Box Domain, AA 9-79, full length Log in to see 19N10B7
13.099335 ABIN4319346 FACS IHC (p) WB Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
13.099335 ABIN4319335 CyTOF FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3
13.099335 ABIN4319340 CyTOF FACS IHC IHC (p) SimWes WB Mouse IgG2b kappa A Box Domain, AA 9-79, full length Log in to see 19N10B7
1 ABIN2656846 FACS Alexa Fluor 594 Mouse IgG2b kappa Log in to see 3E8 7
1 ABIN2474000 FACS IF WB Hamster IgG Log in to see HMG1-5H6 1
1 ABIN1735426 ICC FACS IF IHC WB Rabbit AA 100-200 Log in to see Polyclonal
1 ABIN2662754 FACS PE Mouse IgG2b kappa Log in to see 3E8 7
1 ABIN1724247 FACS IHC (p) WB Mouse IgG1 kappa full length Log in to see IMG19N15F4
1 ABIN1724246 FACS IHC (p) WB Mouse IgG2b kappa full length Log in to see IMG19N10B7
1 ABIN4903942 FACS ICC IF IHC WB Rabbit IgG Log in to see
1 ABIN4319375 FACS IHC (p) WB DyLight 488 Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
1 ABIN4319376 FACS IHC (p) WB DyLight 755 Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
1 ABIN4319377 FACS IHC (p) WB PerCP Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
1 ABIN4319378 FACS PE Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
1 ABIN4319379 FACS IHC (p) WB DyLight 550 Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
1 ABIN4319345 FACS IHC (p) WB Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
1 ABIN4319380 FACS IHC (p) WB DyLight 350 Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4
1 ABIN4319381 FACS IHC (p) WB DyLight 405 Mouse IgG1 kappa A Box Domain, AA 9-79, full length Log in to see 19N15F4

Top referenced anti-HMGB1 antibody for Flow Cytometry

Similar anti-HMGB1 Antibodies

Application / Reactivity Sheep (Ovine) Rat (Rattus)
ELISA 12 Antibodies 58 Antibodies
Flow Cytometry (FACS) 12 Antibodies 36 Antibodies
Immunocytochemistry (ICC) 12 Antibodies 36 Antibodies
Immunofluorescence (IF) 12 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 13 Antibodies 39 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies 31 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 12 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope AA 100-200, Internal Region
(67), (53), (37), (37), (37), (19), (18), (18), (18), (17), (13), (13), (12), (12), (11), (11), (7), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)
(425), (204), (138), (50), (39), (29), (13), (12), (6), (6), (3), (1), (1)
Host Rabbit
(235), (199), (4), (4), (2), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(27), (18), (18), (16), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (5), (5), (5), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Simple Western (SimWes), Western Blotting (WB)
(358), (192), (130), (120), (101), (82), (44), (15), (11), (11), (6), (6), (5), (2), (1), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN250703) Images
Cross-Reactivity (Details) Predicted to react with porcine, equine,rabbit based on 100% sequence homology.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to an internal portion of the human HMGB1 protein sequence (between residues 100-200). [UniProt #P09429]

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN250703) Images back to top
Antigen
Alternative Name HMGB1/HMG-1 (HMGB1 Antibody Abstract)
Background Gene Symbol: HMGB1
Molecular Weight Theoretical MW: 29 kDa
Gene ID 3146
UniProt P09429
Pathways p53 Signaling

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN250703) Images back to top
Application Notes Western Blot 0.5-1.0 μg/mL, Simple Western 1:2000, Flow Cytometry 1:100, ELISA, Immunohistochemistry 1:100-1:250, Immunocytochemistry/Immunofluorescence 0.05 μg/mL, Immunohistochemistry-Paraffin 1:100-1:250This HMGB1 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western blot, where a band is seen approx. 29 kDa. It has been reported that the HRP conjugated format (Catalog# NB100-2322H) of this antibody works well for use in ELISA. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol WB Protocol specific for HMGB1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 35 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 2 hours at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-HMGB1 primary antibody in blocking buffer and incubate 3 hours at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 2 hours at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce's ECL).Note: Tween-20 can be added to the blocking or antibody diultion buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.IHC-P Protocol specific for HMGB1 Antibody Immunohistochemistry-paraffin embedded sectionsAntigen UnmaskingBring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench top for 30 minutes. Staining
. Wash sections in dH2O three times for 5 minutes each.
. Wash section in wash buffer (1X PBS/0.1 % Tween-20 (1X PBST)) for 5 minutes.
. Block each section with 100-400 µL blocking solution (1X PBST, 5 % goat serum) for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL primary antibody diluted in 1X PBST, 5 % goat serum to each section. Incubate overnight at 4C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated secondary antibody, diluted in 1X PBST, 5 % goat serum. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Striptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in dH2O.
. Counterstain sections in hematoxylin.
. Wash sections in dH2O two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN250703) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-HMGB1 Antibody (ABIN250703)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Kim, Lee, Oh, Park, Park, Hong, Kim, Lee, Ko, Choi, Hong, Jang: "Development of Advanced Atherosclerotic Plaque by Injection of Inflammatory Proteins in a Rabbit Iliac Artery Model." in: Yonsei medical journal, Vol. 57, Issue 5, pp. 1095-105, 2016 (Sample species: Rabbit). Further details: Immunohistochemistry

Zhou, Wen, Cheng, Ma, Li, Ren, Sheng, Dong, Lu, Hu, Wang: "Tumor-released autophagosomes induce IL-10-producing B cells with suppressive activity on T lymphocytes via TLR2-MyD88-NF-κB signal pathway." in: Oncoimmunology, Vol. 5, Issue 7, pp. e1180485, 2016 Further details: Flow Cytometry

Feng, Yang, Ma, Bai, Chen: "Role of Toll-like receptors in diabetic renal lesions in a miniature pig model." in: Science advances, Vol. 1, Issue 5, pp. e1400183, 2015 (Sample species: Cow (Bovine)). Further details: Immunohistochemistry

Lu, Huang, Liao, Yen: "Ursolic acid triggers nonprogrammed death (necrosis) in human glioblastoma multiforme DBTRG-05MG cells through MPT pore opening and ATP decline." in: Molecular nutrition & food research, Vol. 58, Issue 11, pp. 2146-56, 2014

Zahedi, Barone, Wang, Murray-Stewart, Roy-Chaudhury, Smith, Casero, Soleimani: "Proximal tubule epithelial cell specific ablation of the spermidine/spermine N1-acetyltransferase gene reduces the severity of renal ischemia/reperfusion injury." in: PLoS ONE, Vol. 9, Issue 11, pp. e110161, 2014 (Sample species: Human). Further details: Western Blotting

Kang, Tang, Schapiro, Loux, Livesey, Billiar, Wang, Van Houten, Lotze, Zeh: "The HMGB1/RAGE inflammatory pathway promotes pancreatic tumor growth by regulating mitochondrial bioenergetics." in: Oncogene, Vol. 33, Issue 5, pp. 567-77, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Robinson, Caldis, Harp, Goings, Miller: "High-mobility group box 1 protein (HMGB1) neutralization ameliorates experimental autoimmune encephalomyelitis." in: Journal of autoimmunity, Vol. 43, pp. 32-43, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Jiang, Scott, Kwak, Chen, Mardon: "Sds22/PP1 links epithelial integrity and tumor suppression via regulation of myosin II and JNK signaling." in: Oncogene, Vol. 30, Issue 29, pp. 3248-60, 2011

Montie, Pestell, Merry: "SIRT1 modulates aggregation and toxicity through deacetylation of the androgen receptor in cell models of SBMA." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 48, pp. 17425-36, 2011

Fazio, Dimattia, Chadi, Kernohan, Pin: "Stanniocalcin 2 alters PERK signalling and reduces cellular injury during cerulein induced pancreatitis in mice." in: BMC cell biology, Vol. 12, pp. 17, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Tang, Kang, Livesey, Kroemer, Billiar, Van Houten, Zeh, Lotze: "High-mobility group box 1 is essential for mitochondrial quality control." in: Cell metabolism, Vol. 13, Issue 6, pp. 701-11, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Barlan, Griffin, McGuire, Wiethoff: "Adenovirus membrane penetration activates the NLRP3 inflammasome." in: Journal of virology, Vol. 85, Issue 1, pp. 146-55, 2010 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN250703) back to top
Supplier Images
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Detection of HMGB1 in whole cell HeLa lysate using ABIN250703. ECL exposure, 1 min.
Immunohistochemistry (IHC) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Staining of HMGB1 in mouse liver using ABIN250703.
Simple Western (SimWes) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Simple Western: HMGB1/HMG-1 Antibody [ABIN250703] - Simple Western lane view shows a ...
ELISA image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) ELISA: HMGB1/HMG-1 Antibody [ABIN250703] - A dose-dependent titration of the HRP conj...
Flow Cytometry (FACS) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Flow Cytometry: HMGB1/HMG-1 Antibody [ABIN250703] - Staining of NTERA-2 cells using A...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Western Blot: HMGB1/HMG-1 Antibody [ABIN250703] - Total protein from SHSY-5Y, MCF7, N...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Western Blot: HMGB1/HMG-1 Antibody [ABIN250703] - Hepatocyte protein lysate at 1:1000...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Western Blot: HMGB1/HMG-1 Antibody [ABIN250703] - HMGB1 antibody was tested in HeLa c...
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (AA 100-200) (ABIN250703) Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody [ABIN250703] - HeLa cell...