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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Western Blotting (WB)||244 Antibodies||86 Antibodies||76 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies||2 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||12 Antibodies||1 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||62 Antibodies||22 Antibodies||18 Antibodies|
|Immunohistochemistry (IHC)||77 Antibodies||28 Antibodies||27 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||4 Antibodies||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Antigen||High-Mobility Group Box 1 (HMGB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This HMGB1 antibody is un-conjugated Alternatives|
Intracellular Flow Cytometry (ICFC)
|7 references available|
|Supplier||Log in to see|
Product Details anti-HMGB1 AntibodyTarget Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2666344) Images
|Purification||The antibody was purified by affinity chromatography.|
Target Details HMGB1Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 Antibody (ABIN2666344) Images back to top
|Alternative Name||HMGB1 (HMGB1 Antibody Abstract)|
|Background||High mobility group protein B1 (HMGB1) belongs to a family of highly conserved proteins that contain HMG box domains. Human HMGB1 is expressed as a 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu. Human HMGB1 is 100 % aa identical to canine HMGB1 and 99 % aa identical to mouse, rat, bovine and porcine HMGB1. HMGB1 is a widely expressed and highly abundant protein. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 also plays a significant role in extracellular signaling associated with inflammation. HMGB1 is massively released into the extracellular environment during cell necrosis. It acts as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction. In addition, activated monocytes, macrophages, and dendritic cells also secrete HMGB1, forming a positive feedback loop that results in the release of additional cytokines and neutrophils. The cytokine activity of HMGB1 is restricted to the HMG B box, while the A box is associated with the helix-loop-helix domain of transcription factors. Although HMGB1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4.|
|Research Area||Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology|
Application DetailsProduct Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 Antibody (ABIN2666344) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 Antibody (ABIN2666344) Images back to top
|Buffer||Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Upon receipt, store undiluted between 2°C and 8°C.|
References for anti-HMGB1 Antibody (ABIN2666344)Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Zhou, Wang, Wang et al.: "Generation of monoclonal antibodies against highly conserved antigens." in: PLoS ONE, Vol. 4, Issue 6, pp. e6087, 2009 (PubMed).
Campana, Bosurgi, Rovere-Querini: "HMGB1: a two-headed signal regulating tumor progression and immunity." in: Current opinion in immunology, Vol. 20, Issue 5, pp. 518-23, 2008 (PubMed).
Klune, Dhupar, Cardinal et al.: "HMGB1: endogenous danger signaling." in: Molecular medicine (Cambridge, Mass.), Vol. 14, Issue 7-8, pp. 476-84, 2008 (PubMed).
Dumitriu, Baruah, Manfredi et al.: "HMGB1: guiding immunity from within." in: Trends in immunology, Vol. 26, Issue 7, pp. 381-7, 2005 (PubMed).
Müller, Ronfani, Bianchi: "Regulated expression and subcellular localization of HMGB1, a chromatin protein with a cytokine function." in: Journal of internal medicine, Vol. 255, Issue 3, pp. 332-43, 2004 (PubMed).
Bonaldi, Talamo, Scaffidi et al.: "Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion." in: The EMBO journal, Vol. 22, Issue 20, pp. 5551-60, 2003 (PubMed).
Thomas, Travers: "HMG1 and 2, and related 'architectural' DNA-binding proteins." in: Trends in biochemical sciences, Vol. 26, Issue 3, pp. 167-74, 2001 (PubMed).
ImagesProduct Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 Antibody (ABIN2666344) back to top