anti-Pig (Porcine) HMGB1 antibody for Immunocytochemistry

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • hmgb1
  • amphoterin
  • hmg-1
  • hmg1
  • hmg3
  • sbp-1
  • HMGB1
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • p30
  • Ac2-008
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • Hmgb1
  • high-mobility group box 1
  • high mobility group box 1
  • high mobility group protein B1-like
  • high-mobility group box 1a
  • HMG-box transcription factor 1
  • hmgb1
  • HMGB1
  • Hmgb1
  • LOC100392312
  • LOC100754755
  • LOC100359149
  • hmgb1a
  • Hbp1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
450
231
160
59
40
37
23
14
13
10
10
10
7
5
3
2
1
Host
Mouse
244
211
3
2
2
2
2
Clonality
Monoclonal
Conjugate
This HMGB1 antibody is un-conjugated
27
20
17
17
9
9
8
8
8
7
7
7
7
6
6
6
5
5
5
4
3
3
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
383
187
137
127
116
85
45
19
13
11
9
8
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN4319335
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4319334 CyTOF FACS ICC IF SimWes WB Mouse IgG2b Log in to see 1F3
1 ABIN4961211 FACS ICC IF WB Alexa Fluor 405 Mouse IgG2b Log in to see 1F3
1 ABIN4961219 FACS ICC IF WB DyLight 680 Mouse IgG2b Log in to see 1F3
1 ABIN4961225 FACS ICC IF WB DyLight 550 Mouse IgG2b Log in to see 1F3
1 ABIN4961218 FACS ICC IF FITC Mouse IgG2b Log in to see 1F3
1 ABIN4961226 FACS ICC IF WB DyLight 350 Mouse IgG2b Log in to see 1F3
1 ABIN4961216 ICC IF WB Biotin Mouse IgG2b Log in to see 1F3
1 ABIN4961217 FACS ICC IF WB DyLight 650 Mouse IgG2b Log in to see 1F3
1 ABIN4961222 FACS ICC IF WB DyLight 755 Mouse IgG2b Log in to see 1F3
1 ABIN4961220 FACS ICC IF WB DyLight 488 Mouse IgG2b Log in to see 1F3
1 ABIN4961227 FACS ICC IF WB DyLight 405 Mouse IgG2b Log in to see 1F3
1 ABIN4961213 FACS ICC IF WB Alexa Fluor 647 Mouse IgG2b Log in to see 1F3
1 ABIN4961214 FACS ICC IF WB Alexa Fluor 700 Mouse IgG2b Log in to see 1F3
1 ABIN4961212 FACS ICC IF WB Alexa Fluor 488 Mouse IgG2b Log in to see 1F3
1 ABIN1580427 ICC IF IHC WB Mouse IgG2b Log in to see 1F3
1 ABIN1959851 ICC IF WB Mouse IgG2b Log in to see 1F3
1 ABIN1951825 ICC IF WB Mouse IgG2b Log in to see

Similar anti-HMGB1 Antibodies

Application / Reactivity Rat (Rattus) Pig (Porcine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies 2 Antibodies
ELISA 58 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 38 Antibodies 17 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 36 Antibodies
Immunofluorescence (IF) 48 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 44 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Simple Western (SimWes) 3 Antibodies
Western Blotting (WB) 126 Antibodies

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(450), (231), (160), (59), (40), (37), (23), (14), (13), (10), (10), (10), (7), (5), (3), (2), (1)
Host Mouse
(244), (211), (3), (2), (2), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HMGB1 antibody is un-conjugated
(27), (20), (17), (17), (9), (9), (8), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Simple Western (SimWes), Western Blotting (WB)
(383), (187), (137), (127), (116), (85), (45), (19), (13), (11), (9), (8), (4), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling Images
Purification Immunogen affinity purified
Immunogen Full length human HMGB1 [UniProt# P09429]
Clone 1F3
Isotype IgG2b

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HMGB1/HMG-1 (HMGB1 Antibody Abstract)
Background Gene Symbol: HMGB1
Molecular Weight Theoretical MW: 25 kDa
Gene ID 3146
UniProt P09429
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling Images back to top
Application Notes Western Blot 1:1000-1:2000, Simple Western 1:500, Flow Cytometry 1 μg per million cells, Immunocytochemistry/Immunofluorescence 1:1000, CyTOF-readyThis HMGB1 (1F3) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band is seen at approx. 25 kDa. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 10 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling back to top
Supplier Images
Simple Western (SimWes) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN4319335) Simple Western: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Simple Western lane view sh...
Western Blotting (WB) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN4319335) Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Blot of crude HeLa cell extra...
Flow Cytometry (FACS) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN4319335) Flow Cytometry: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Intracellular flow cytometr...
Immunofluorescence (IF) image for anti-HMGB1 antibody (High-Mobility Group Box 1) (ABIN4319335) Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - HeL...