anti-Rat (Rattus) HMGB1 antibody for Western Blotting

Recommended HMGB1 Antibody (supplied by: Log in to see )

Antigen
High-Mobility Group Box 1 (HMGB1) Antibodies
  • hmgb1
  • amphoterin
  • hmg-1
  • hmg1
  • hmg3
  • sbp-1
  • HMGB1
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • p30
  • Ac2-008
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • Hmgb1
  • high-mobility group box 1
  • high mobility group box 1
  • high mobility group protein B1-like
  • high-mobility group box 1a
  • HMG-box transcription factor 1
  • hmgb1
  • HMGB1
  • Hmgb1
  • LOC100392312
  • LOC100754755
  • LOC100359149
  • hmgb1a
  • Hbp1
Epitope
N-Term
69
61
38
38
32
30
20
19
19
16
15
15
12
11
11
11
8
6
5
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
460
232
161
59
41
36
22
14
13
10
9
9
7
5
3
2
1
Host
Rabbit
244
221
3
2
2
2
2
Clonality
Polyclonal
Conjugate
This HMGB1 antibody is un-conjugated
27
20
18
17
10
10
9
9
9
7
7
7
7
6
6
6
5
5
5
5
4
4
1
1
1
1
1
1
1
Application
Western Blotting (WB)
385
187
137
128
127
86
48
21
13
12
9
9
8
3
2
2
1
1
1
1
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Catalog No. ABIN2780399
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1229854 ABIN2776383 WB Rabbit Middle Region Log in to see Polyclonal 1
3.1229854 ABIN2780400 WB Rabbit Middle Region Log in to see Polyclonal 1
3.1229854 ABIN2787912 WB Rabbit Middle Region Log in to see Polyclonal
3.1229854 ABIN2787911 WB Rabbit N-Term Log in to see Polyclonal
3.1229854 ABIN1533598 ELISA WB Rabbit IgG AA 131-180 Log in to see Polyclonal 2
3.1229854 ABIN250703 ELISA FACS ICC IF IHC IHC (p) SimWes WB Rabbit AA 100-200, Internal Region Log in to see Polyclonal 13
3.1229854 ABIN1449284 WB Mouse IgG Internal Region Log in to see 4C9 3
3.1229854 ABIN671616 IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 7
3.1229854 ABIN1107584 WB Mouse IgG2a Log in to see KS1 1
3.1229854 ABIN2474000 FACS IF WB Hamster IgG Log in to see HMG1-5H6 1
3.1229854 ABIN1169270 ELISA WB Human IgG2 Log in to see Giby-1-4
3.1229854 ABIN2854997 ICC IF IHC (p) IHC WB Rabbit IgG Center Log in to see Polyclonal
3.1229854 ABIN2862626 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.1229854 ABIN2957615 WB Rabbit N-Term, acLys12 Log in to see Polyclonal
3.1229854 ABIN2957616 IHC WB Rabbit N-Term Log in to see Polyclonal
3.1229854 ABIN3185048 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1229854 ABIN2506663 ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal
3.1229854 ABIN1873039 IHC IP WB Rabbit IgG Log in to see Polyclonal
3.1229854 ABIN1873041 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.1229854 ABIN2957614 IC IF IHC WB Rabbit Log in to see Polyclonal

General

Antigen High-Mobility Group Box 1 (HMGB1) Antibodies
Epitope N-Term
(69), (61), (38), (38), (32), (30), (20), (19), (19), (16), (15), (15), (12), (11), (11), (11), (8), (6), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(460), (232), (161), (59), (41), (36), (22), (14), (13), (10), (9), (9), (7), (5), (3), (2), (1)
Host Rabbit
(244), (221), (3), (2), (2), (2), (2)
Clonality
Polyclonal
Conjugate This HMGB1 antibody is un-conjugated
(27), (20), (18), (17), (10), (10), (9), (9), (9), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(385), (187), (137), (128), (127), (86), (48), (21), (13), (12), (9), (9), (8), (3), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-HMGB1 Antibody

Target Details HMGB1 Application Details Handling References for anti-HMGB1 antibody (ABIN2780399) Images
Blocking Peptide Blocking peptide for this product available: ABIN978051
Sequence GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against HMGB1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human HMGB1

Target Details HMGB1

Product Details anti-HMGB1 Antibody Application Details Handling References for anti-HMGB1 antibody (ABIN2780399) Images back to top
Antigen
Alternative Name HMGB1 (HMGB1 Antibody Abstract)
Background HMGB1 belongs to the HMGB family. It contains 2 HMG box DNA-binding domains. The protein binds preferentially single-stranded DNA and unwinds double stranded DNA. The oxidized HMGB1 may accumulate even in cells under oxidative stress.
Alias Symbols: DKFZp686A04236, HMG1, HMG3, SBP-1
Protein Interaction Partner: AGTRAP, HUWE1, UBC, ASB2, ASB1, ZNF24, MAPK1, HMGA1, ITGA4, FN1, HNF1A, SSRP1, SMARCE1, PSEN1, XPC, XPA, RAD23B, CDK2, TFAP4, TERF2IP, TERF2, HHV8GK18_gp81, SUMO2, FCP1, DNM2, HSPA8, HMGB2, PDIA3, Ccdc15, POU5F1, H3F3A, CREBBP, CDK1, TBP, LRIF1, RASSF4, N
Protein Size: 215
Molecular Weight 25 kDa
Gene ID 3146
NCBI Accession NM_002128, NP_002119
UniProt P09429
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Chromatin Binding Proteins, Neurology
Pathways p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration

Application Details

Product Details anti-HMGB1 Antibody Target Details HMGB1 Handling References for anti-HMGB1 antibody (ABIN2780399) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 215 AA

Restrictions For Research Use only

Handling

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details References for anti-HMGB1 antibody (ABIN2780399) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HMGB1 antibody (ABIN2780399)

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling Images back to top
Product cited in:

Straino, Di Carlo, Mangoni, De Mori, Guerra, Maurelli, Panacchia, Di Giacomo, Palumbo, Di Campli, Uccioli, Biglioli, Bianchi, Capogrossi, Germani: "High-mobility group box 1 protein in human and murine skin: involvement in wound healing." in: The Journal of investigative dermatology, Vol. 128, Issue 6, pp. 1545-53, 2008

Images

Product Details anti-HMGB1 Antibody Target Details HMGB1 Application Details Handling References for anti-HMGB1 antibody (ABIN2780399) back to top
Supplier Images
Western Blotting (WB) image for anti-High-Mobility Group Box 1 (HMGB1) (N-Term) antibody (ABIN2780399) WB Suggested Anti-HMGB1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: