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|Antigen||High-Mobility Group Box 1 (HMGB1) ELISA Kits|
Kits with alternative reactivity to:
|Method Type||Sandwich ELISA|
|Detection Range||1.56 ng/mL - 100 ng/mL|
|Minimum Detection Limit||1.56 ng/mL|
|20 references available|
|Supplier||Log in to see|
Product Details HMGB1 ELISA KitTarget Details Application Details Handling References for HMGB1 Kit (ABIN414391) Images
|Purpose||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HMG1 in Serum,Plasma,Biological Fluids|
|Sample Type||Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate|
This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1).
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.|
|Material not included||
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|Alternative Name||HMG1 (HMGB1 ELISA Kit Abstract)|
|Pathways||p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration|
Application DetailsProduct Details HMGB1 ELISA Kit Target Details Handling References for HMGB1 Kit (ABIN414391) Images back to top
Information on standard material:
|Sample Volume||100 μL|
|Assay Time||3 h|
|Protocol||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Protein 1 (HMG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group Protein 1 (HMG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Construct a standard curve by plotting the mean O.D. and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with HMG1 concentration on the y-axis and absorbance on the x-axis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested 20 times on one plate, respectively.
|Restrictions||For Research Use only|
HandlingProduct Details HMGB1 ELISA Kit Target Details Application Details References for HMGB1 Kit (ABIN414391) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|Expiry Date||6 months|
References for HMGB1 Kit (ABIN414391)Product Details HMGB1 ELISA Kit Target Details Application Details Handling Images back to top
|Product cited in:||
Chen, Fang, Li, Chen, Li, Gong, Fang: "Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells." in: Immunologic research, Vol. 65, Issue 3, pp. 666-680, 2017
Yin, Feng, Zhao, Zhao, Yua, Xu, Che: "SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death." in: Biomedicine & pharmacotherapy, Vol. 88, pp. 449-458, 2017
Gmiat, Mieszkowski, Prusik, Prusik, Kortas, Kochanowicz, Radulska, Lipiński, Tomczyk, Jaworska, Antosiewicz, Ziemann: "Changes in pro-inflammatory markers and leucine concentrations in response to Nordic Walking training combined with vitamin D supplementation in elderly women." in: Biogerontology, Vol. 18, Issue 4, pp. 535-548, 2017
Ying, Jiang, He, Yu, Chen, Chen, Ru, Chen, Chen, Zhu, Li, Zhang, Guo, Yin, Zhang, Lou: "Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases." in: Disease markers, Vol. 2017, pp. 5756102, 2017
Kargı, Demirpençe, Gündüz, Göktaş, Alikanoǧlu, Yıldırım: "Serum levels of HMGB1 have a diagnostic role in metastatic renal cell cancer." in: Cancer biomarkers : section A of Disease markers, Vol. 17, Issue 1, pp. 17-20, 2016
Ming, Gao, Zou, Chen, Sun, Xiao, Lai, Xiong, Xu, Tan, Wang, Chen, Gong, Xia, Zheng: "HMGB1 blockade differentially impacts pulmonary inflammation and defense responses in poly(I:C)/LPS-exposed heart transplant mice." in: Molecular immunology, Vol. 76, pp. 80-9, 2016
Wang, Sun, Li, Deng, Zeng, Tao, Wang, Guan, Zhao: "Urinary MCP-1、HMGB1 increased in calcium nephrolithiasis patients and the influence of hypercalciuria on the production of the two cytokines." in: Urolithiasis, Vol. 45, Issue 2, pp. 159-175, 2016
Wang, Wei, Liu, Zhou, Xiao, Dong, Li: "The Role of High Mobility Group Box 1 Protein in Interleukin-18-Induced Myofibroblastic Transition of Valvular Interstitial Cells." in: Cardiology, Vol. 135, Issue 3, pp. 168-178, 2016
Wang, Li, Yu, Guo, Yang, Zhang, Li, Li, Hu, Zheng, Song: "High Mobility Group Box 1 Mediates Interferon-γ-Induced Phenotypic Modulation of Vascular Smooth Muscle Cells." in: Journal of cellular biochemistry, Vol. 118, Issue 3, pp. 518-529, 2016
Wang, Miao, Wu, Huang, Sun, Zhu, Zheng, Sun, Dong: "Serum HMGB1 Serves as a Novel Laboratory Indicator Reflecting Disease Activity and Treatment Response in Ankylosing Spondylitis Patients." in: Journal of immunology research, Vol. 2016, pp. 6537248, 2016
Basso, Padoan, Laufer, Aneloni, Moz, Schroers, Pelloso, Saiz, Krapp, Fogar, Cornoldi, Zambon, Rossi, La Malfa, Marotti, Brefort, Weis, Katus, Plebani: "Relevance of pre-analytical blood management on the emerging cardiovascular protein biomarkers TWEAK and HMGB1 and on miRNA serum and plasma profiling." in: Clinical biochemistry, Vol. 50, Issue 4-5, pp. 186-193, 2016
Alizadeh, Ghazavi, Ganji, Mosayebi: "Diagnostic Value of High-Mobility Group Box 1 (HMGB1) Protein in Acute and Perforated Appendicitis." in: Journal of investigative surgery : the official journal of the Academy of Surgical Research, pp. 1-5, 2016
Kwon, Kim, Park, Choi, Lee, Lee, Heo, Chang, Koh: "Increased VEGF and decreased SDF-1? in patients with silent brain infarction are associated with better prognosis after first-ever acute lacunar stroke." in: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, Vol. 24, Issue 3, pp. 704-10, 2015
Qin, Mi, Li, Wang, Zhang, Wang, Zhang, Ma, Wu, Zhang: "Low shear stress induced HMGB1 translocation and release via PECAM-1/PARP-1 pathway to induce inflammation response." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120586, 2015
Qu, Wang, Huang, Qiu, Xiang, Lu: "High mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgery." in: Journal of cardiothoracic surgery, Vol. 10, pp. 88, 2015
Wang, Pei, Zhu, Zhou, Liu, Xiong, Liu, Lin, Xie: "Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro." in: World journal of gastroenterology, Vol. 21, Issue 25, pp. 7764-76, 2015
Xiao, Wu, Yin, Han, Ding, Qiao, Lu, Deng, Bo, Gong: "Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity." in: Journal of immunotherapy (Hagerstown, Md. : 1997), Vol. 38, Issue 5, pp. 167-84, 2015
Wang, Qu, Deng: "Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in China." in: PLoS ONE, Vol. 10, Issue 8, pp. e0136564, 2015
Young, Huang, Wu, Lai, Hou, Peng, Liang, Lin, Chang, Tsai, Wu, Wu, Ko: "Hemojuvelin modulates iron stress during acute kidney injury: improved by furin inhibitor." in: Antioxidants & redox signaling, Vol. 20, Issue 8, pp. 1181-94, 2014
Iwamoto, Gao, Pulkkinen, Toljamo, Nieminen, Mazur: "Soluble receptor for advanced glycation end-products and progression of airway disease." in: BMC pulmonary medicine, Vol. 14, pp. 68, 2014
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