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|Application / Reactivity||Human|
|ELISA||8 ELISA Kits|
|Antigen||High-Mobility Group Box 1 (HMGB1) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Sandwich ELISA|
|Detection Range||62.5-4000 pg/mL|
|Minimum Detection Limit||62.5 pg/mL|
|21 references available|
|Supplier||Log in to see|
Product Details HMGB1 ELISA KitTarget details Application Details Handling References for HMGB1 Kit (ABIN414391) Images
|Purpose||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HMG1 in Serum,Plasma,Biological Fluids|
|Sample Type||Serum, Plasma, Biological Fluids|
|Specificity||This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1).|
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.|
The minimum detectable dose of HMG1 is typically less than 32.2 pg/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration
that could be differentiated from zero. It was determined by adding two standard deviations - the mean optical
density value of twenty zero standard replicates and calculating the corresponding concentration.
|Material not included||
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|Alternative Name||HMG1 (HMGB1 ELISA Kit Abstract)|
Application DetailsProduct Details HMGB1 ELISA Kit Target details Handling References for HMGB1 Kit (ABIN414391) Images back to top
Information on standard material:
|Sample Volume||100 μL|
|Assay Time||4.5 h|
|Plate||Pre-coated,Strips (12 x 8)|
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HMG1.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
After TMB substrate solution is added, only those wells that contain HMG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 10 nm. The concentration of HMG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Construct a standard curve by plotting the mean O.D. and concentration for each standard and draw a best fit curve through the points on the graph or create a standard curve on log-log graph paper with HMG1 concentration on the y-axis and absorbance on the x-axis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (precision within an assay): 3 samples with low, middle and high level HMG1 were tested 20 times on one plate, respectively.
Inter-assay Precision (precision between assays): 3 samples with low, middle and high level HMG1 were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/mean X 100
|Restrictions||For Research Use only|
HandlingProduct Details HMGB1 ELISA Kit Target details Application Details References for HMGB1 Kit (ABIN414391) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
|Handling Advice||To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.|
|Storage||4 °C/-20 °C|
|Expiry Date||6 months|
References for HMGB1 Kit (ABIN414391)Product Details HMGB1 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Wang, Qu, Deng: "Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in China." in: PLoS ONE, Vol. 10, Issue 8, pp. e0136564, 2015 (PubMed).
Wang, Pei, Zhu et al.: "Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro." in: World journal of gastroenterology, Vol. 21, Issue 25, pp. 7764-76, 2015 (PubMed).
Qu, Wang, Huang et al.: "High mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgery." in: Journal of cardiothoracic surgery, Vol. 10, pp. 88, 2015 (PubMed).
Xiao, Wu, Yin et al.: "Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity." in: Journal of immunotherapy (Hagerstown, Md. : 1997), Vol. 38, Issue 5, pp. 167-84, 2015 (PubMed).
Qin, Mi, Li et al.: "Low shear stress induced HMGB1 translocation and release via PECAM-1/PARP-1 pathway to induce inflammation response." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120586, 2015 (PubMed).
Kwon, Kim, Park et al.: "Increased VEGF and decreased SDF-1? in patients with silent brain infarction are associated with better prognosis after first-ever acute lacunar stroke." in: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association, Vol. 24, Issue 3, pp. 704-10, 2015 (PubMed).
Fraisier, Papa, Almeras: "High-mobility group box-1, promising serological biomarker for the distinction of human WNV disease severity." in: Virus research, Vol. 195, pp. 9-12, 2014 (PubMed).
Wang, Karki, Zhao et al.: "High plasma levels of high mobility group box 1 is associated with the risk of sepsis in severe blunt chest trauma patients: a prospective cohort study." in: Journal of cardiothoracic surgery, Vol. 9, pp. 133, 2014 (PubMed).
Wang, Karki, Du et al.: "Plasma levels of high mobility group box 1 increase in patients with posttraumatic stress disorder after severe blunt chest trauma: a prospective cohort study." in: The Journal of surgical research, Vol. 193, Issue 1, pp. 308-15, 2014 (PubMed).
Iwamoto, Gao, Pulkkinen et al.: "Soluble receptor for advanced glycation end-products and progression of airway disease." in: BMC pulmonary medicine, Vol. 14, pp. 68, 2014 (PubMed).
Thierry, Giraud, Robin et al.: "The alarmin concept applied to human renal transplantation: evidence for a differential implication of HMGB1 and IL-33." in: PLoS ONE, Vol. 9, Issue 2, pp. e88742, 2014 (PubMed).
Lai, Cheng, Lin et al.: "ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 Expression." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2013, pp. 716481, 2013 (PubMed).
Yao, Zhao, Han et al.: "Correlation between serum high-mobility group box-1 levels and high-sensitivity C-reactive protein and troponin I in patients with coronary artery disease." in: Experimental and therapeutic medicine, Vol. 6, Issue 1, pp. 121-124, 2013 (PubMed).
Young, Huang, Wu et al.: "Hemojuvelin modulates iron stress during acute kidney injury: improved by furin inhibitor." in: Antioxidants & redox signaling, Vol. 20, Issue 8, pp. 1181-94, 2014 (PubMed).
Russo: "Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism." in: Biomarker insights, Vol. 8, pp. 35-41, 2013 (PubMed).
Ayarc?, Y?lmaz, S???rl? et al.: "Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosis." in: Microbiology and immunology, Vol. 57, Issue 2, pp. 150-8, 2013 (PubMed).
Oktayoglu, Em, Tahtasiz et al.: "Elevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity and quality of life." in: Rheumatology international, Vol. 33, Issue 5, pp. 1327-31, 2013 (PubMed).
Eskici, Aç?kgöz, Pi?kin et al.: "High mobility group B1 levels in sepsis and Disseminated Intravascular Coagulation." in: Acta biochimica Polonica, Vol. 59, Issue 4, pp. 561-6, 2013 (PubMed).
Siddik: "Minimising the organ toxic effects of chemotherapy." in: European journal of cancer & clinical oncology, Vol. 22, Issue 8, pp. 905-7, 1986 (PubMed).
Prasad, Mahajan, Ganguly: "Effect of chloroquine on cellular immune responses of normal and P. knowlesi-infected rhesus monkeys." in: Immunology and cell biology, Vol. 65 ( Pt 3), pp. 211-6, 1987 (PubMed).
Khaĭtov: "[Treatment of acute viral hepatitis with cortisone]." in: Vŭtreshni bolesti, Vol. 25, Issue 3, pp. 39-43, 1986 (PubMed).
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