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anti-Human HRAS antibody for Immunofluorescence

Recommended HRAS Antibody (supplied by: Log in to see )

Antigen
V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Antibodies
  • AI929937
  • c-bas/has
  • C-BAS/HAS
  • C-H-RAS
  • c-H-ras
  • c-Ha-ras
  • C-HA-RAS1
  • c-rasHa
  • CTLO
  • H-RAS
  • H-Ras
  • H-ras
  • H-RASIDX
  • Ha-ras
  • HAMSV
  • Harvey-ras
  • HRAS
  • hras
  • Hras-1
  • HRAS1
  • hras1
  • K-ras
  • K-Ras
  • K-RAS
  • Ki-ras
  • Kras-2
  • Kras2
  • N-RAS
  • N-Ras
  • p21B
  • ras
  • rash1
  • RASH1
  • zgc:110250
Epitope
AA 146-176, C-Term
17
8
5
5
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
106
58
54
5
4
2
Host
Rabbit
93
30
2
1
Clonality
Polyclonal
Conjugate
This HRAS antibody is un-conjugated
7
6
6
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
89
42
26
22
18
16
5
4
3
2
2
1
1
1
Supplier
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Catalog No. ABIN655622
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.31819236 ABIN952792 EIA IF WB Rabbit Ig C-Term, AA 153-184 Log in to see Polyclonal 5
0.31819236 ABIN189394 ICC FACS IF IHC (p) IP IHC WB Rabbit IgG C-Term Log in to see Y132
0.31819236 ABIN2440517 ICC IF IHC (fp) WB Rabbit IgG Log in to see Polyclonal
0.31819236 ABIN2438875 IF IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.31819236 ABIN2439724 IF WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.0010830762 ABIN392183 IF IHC (p) WB Rabbit Ig AA 104-128, Center Log in to see Polyclonal 2
0.0010830762 ABIN1498717 IF WB Mouse IgG1 Log in to see 1G8
0.0010830762 ABIN1498715 IF IHC (p) WB Mouse IgG1 Log in to see 1A1
0.0010830762 ABIN1498716 IF IHC (p) WB Mouse IgG1 Log in to see 2A1
0.0010830762 ABIN1498713 IF IHC (p) WB Mouse IgG2b Log in to see 1C2
0.0010830762 ABIN1498714 IF IHC (p) WB Mouse IgG1 Log in to see 1D9
0.0010830762 ABIN224929 IHC (fro) IF IHC (p) IP Neut WB Rat IgG1, kappa AA 62-76 Log in to see
0.0010830762 ABIN1811981 IF WB Rabbit AA 153-184 Log in to see Polyclonal
0.0010830762 ABIN2622046 IF Mouse IgG1 Log in to see 1F2
0.0010830762 ABIN2622047 IF Mouse IgG1 Log in to see 1C8
0.0010830762 ABIN2285765 IF ELISA WB Rabbit IgG C-Term, AA 153-184 Log in to see Polyclonal
0.0010830762 ABIN1840128 IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
0.0010830762 ABIN2741464 IF IP IHC WB Rat IgG1, kappa Log in to see Y13-259
0.0010830762 ABIN3029525 IF IHC ELISA WB Rabbit Ig Fraction AA 104-128 Log in to see Polyclonal
0.0010830762 ABIN2670877 IF IHC WB Mouse IgG2b Log in to see OTIC2

Top referenced anti-HRAS antibody for Immunofluorescence

Similar anti-HRAS Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 19 Antibodies 27 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 5 Antibodies
Immunofluorescence (IF) 8 Antibodies 28 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 22 Antibodies
Immunohistochemistry (IHC) 10 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 35 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies

General

Antigen V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog (HRAS) Antibodies
Epitope AA 146-176, C-Term
(17), (8), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(106), (58), (54), (5), (4), (2)
Host Rabbit
(93), (30), (2), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This HRAS antibody is un-conjugated
(7), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(89), (42), (26), (22), (18), (16), (5), (4), (3), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-HRAS Antibody

Target Details HRAS Application Details Handling References for anti-HRAS Antibody (ABIN655622) Images
Specificity This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-184 amino acids from the C-terminal region of human HRAS.
Predicted Reactivity Chicken,Rat (Rattus)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-176 AA from the C-terminal region of human HRAS.
Clone RB14565
Isotype Ig

Target Details HRAS

Product Details anti-HRAS Antibody Application Details Handling References for anti-HRAS Antibody (ABIN655622) Images back to top
Antigen
Alternative Name HRAS (HRAS Antibody Abstract)
Background This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.
Synonyms: RB14565
Pathways: Neurotrophin signaling pathway, Hepatitis C, Insulin signaling pathway, Axon guidance, Regulation of actin cytoskeleton, MAPK signaling pathway, VEGF signaling pathway, Fc epsilon RI signaling pathway, Long-term depression, GnRH signaling pathway, Pathways in cancer, Non-small cell lung cancer, Chemokine signaling pathway, Natural killer cell mediated cytotoxicity, Focal adhesion, Endocytosis, T cell receptor signaling pathway, B cell receptor signaling pathway, Prostate cancer, Melanoma, Gap junction, Melanogenesis, Tight junction, Bladder cancer, Renal cell carcinoma, ErbB signaling pathway, Chronic myeloid leukemia, Endometrial cancer, Acute myeloid leukemia, Glioma, Thyroid cancer
Molecular Weight 21298 Da
Gene ID 3265
UniProt P01112
Research Area Signaling, Cell Structure, Protein Modifications, Cancer, Chromatin
Pathways p53 Signaling, MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-HRAS Antibody Target Details HRAS Handling References for anti-HRAS Antibody (ABIN655622) Images back to top
Application Notes WB = 1:1000, IF = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-HRAS Antibody Target Details HRAS Application Details References for anti-HRAS Antibody (ABIN655622) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-HRAS Antibody (ABIN655622)

Product Details anti-HRAS Antibody Target Details HRAS Application Details Handling Images back to top
Background publications

Ma, Liu, Wu, Terada: "p66(Shc) restrains Ras hyperactivation and suppresses metastatic behavior." in: Oncogene, Vol. 29, Issue 41, pp. 5559-67, 2010

van Engen-van Grunsven, van Dijk, Ruiter, Klaasen, Mooi, Blokx: "HRAS-mutated Spitz tumors: A subtype of Spitz tumors with distinct features." in: The American journal of surgical pathology, Vol. 34, Issue 10, pp. 1436-41, 2010

Li, Wang, Liu, Paulson, Yee, Zhang: "Transcriptional factor HBP1 targets P16(INK4A), upregulating its expression and consequently is involved in Ras-induced premature senescence." in: Oncogene, Vol. 29, Issue 36, pp. 5083-94, 2010

Kwack, Song, Pyun, Lee, Cho: "[Study on association between an H-RAS gene polymorphism and gastric cancer development]." in: The Korean journal of gastroenterology = Taehan Sohwagi Hakhoe chi, Vol. 56, Issue 2, pp. 78-82, 2010

Images

Product Details anti-HRAS Antibody Target Details HRAS Application Details Handling References for anti-HRAS Antibody (ABIN655622) back to top
Supplier Images
Western Blotting (WB) image for anti-HRAS antibody (V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog) (AA 146-176) (ABIN655622) Western blot analysis of HRAS (arrow) using rabbit polyclonal HRAS Antibody (C-term) ...
Western Blotting (WB) image for anti-HRAS antibody (V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog) (AA 146-176) (ABIN655622) HRAS Antibody (C-term) western blot analysis in mouse cerebellum tissue lysates (35u...
Immunofluorescence (IF) image for anti-HRAS antibody (V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog) (AA 146-176) (ABIN655622) Confocal immunofluorescent analysis of HRAS Antibody (C-term) with MCF-7 cell followe...