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anti-Human MAP4 antibody for Western Blotting

Recommended MAP4 Antibody (supplied by: Log in to see )

Antigen
Microtubule-Associated Protein 4 (MAP4) Antibodies
  • AA407148
  • MAP-4
  • Mtap-4
  • Mtap4
  • mtap4
  • XMAP4
Epitope
AA 583-702
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human
49
31
30
Host
Mouse
49
3
Clonality
Monoclonal
Conjugate
This MAP4 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
24
19
16
14
10
9
6
2
2
1
Supplier
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Catalog No. ABIN968326
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN2788397 WB Rabbit N-Term Log in to see Polyclonal
0.13341247 ABIN754243 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.13341247 ABIN948105 ELISA WB Mouse IgG2a kappa AA 1-99, full length Log in to see 7C9
0.13341247 ABIN926456 WB Rabbit N-Term Log in to see Polyclonal
0.0011728023 ABIN611414 WB Rabbit N-Term Log in to see Polyclonal
0.0011728023 ABIN802068 IF (p) IHC (p) WB Rabbit IgG pSer941 Log in to see Polyclonal
0.0011728023 ABIN793025 ELISA WB Mouse IgG2a, kappa Log in to see 7C9
0.0011728023 ABIN596435 ICC WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN207306 ELISA WB Rabbit pSer941 Log in to see Polyclonal
0.0011728023 ABIN802083 IF (p) IHC (p) WB Rabbit IgG pSer1046 Log in to see Polyclonal
0.0011728023 ABIN802503 IF (p) IHC (p) WB Rabbit IgG pSer1073 Log in to see Polyclonal
0.0011728023 ABIN754245 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN754252 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN261693 IHC (p) IP IHC WB Rabbit IgG AA 1102-1152 Log in to see Polyclonal
0.0011728023 ABIN1088654 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1088653 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1911810 IP WB Rabbit IgG AA 300-350 Log in to see Polyclonal
0.0011728023 ABIN2307124 IC WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN2307125 ELISA WB Mouse IgG2a kappa AA 1-99 Log in to see 7C9
0.0011728023 ABIN1869085 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MAP4 antibody for Western Blotting

Similar anti-MAP4 Antibodies

Application / Reactivity Human
ELISA 18 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 14 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 25 Antibodies

General

Antigen Microtubule-Associated Protein 4 (MAP4) Antibodies
Epitope AA 583-702
(3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(49), (31), (30)
Host Mouse
(49), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MAP4 antibody is un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(24), (19), (16), (14), (10), (9), (6), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-MAP4 Antibody

Target Details MAP4 Application Details Handling References for anti-MAP4 Antibody (ABIN968326) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human MAP4 aa. 583-702
Clone 18-MAP4
Isotype IgG1

Target Details MAP4

Product Details anti-MAP4 Antibody Application Details Handling References for anti-MAP4 Antibody (ABIN968326) Images back to top
Antigen
Alternative Name MAP4 (MAP4 Antibody Abstract)
Background The microtubule (MT) cytoskeleton functions in cytoplasmic organization, cellular movement, determination of cell polarity, intracellular transport, and chromosome segregation. Dynamic instability, continuous cycles of MT assembly (stabilization) and disassembly, mediates MT participation in these events. The function of MTs, particularly stabilization, is regulated by MT-associated proteins (MAPs). A subfamily of MAPs, called AP-MAPs (assembly promoting MAPs), includes tau, MAP2, and MAP4. These proteins are classified as type II MAPs and contain a C-terminal MT binding domain with 3 to 5 imperfect repeats of an 18 amino acid motif. While tau and MAP2 are specifically expressed in neuronal cells, MAP4 is the major MAP of nonneuronal mammalian cells. MAP activity and interaction with MTs are regulated by MARK (MAP/MT affinity-regulating kinase) and mapmodulin. MARK-mediated phosphorylation of MAPs on their homologous KXGS motifs results in detachment of MAPs from MTs and MT disruption. In addition, mapmodulin tightly interacts with the MT-binding domains of MAPs and hinders MAP binding to MTs. Thus, MAP4 is a highly regulated type II MAP that controls MT assembly and disassembly.
Molecular Weight 200-220 kDa
Pathways p53 Signaling, Microtubule Dynamics

Application Details

Product Details anti-MAP4 Antibody Target Details MAP4 Handling References for anti-MAP4 Antibody (ABIN968326) Images back to top
Restrictions For Research Use only

Handling

Product Details anti-MAP4 Antibody Target Details MAP4 Application Details References for anti-MAP4 Antibody (ABIN968326) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-MAP4 Antibody (ABIN968326)

Product Details anti-MAP4 Antibody Target Details MAP4 Application Details Handling Images back to top
Product cited in:

Drewes, Ebneth, Preuss, Mandelkow, Mandelkow: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." in: Cell, Vol. 89, Issue 2, pp. 297-308, 1997

Ulitzur, Humbert, Pfeffer: "Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5084-9, 1997

Chapin, Lue, Yu, Bulinski: "Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains." in: Biochemistry, Vol. 34, Issue 7, pp. 2289-301, 1995

Images

Product Details anti-MAP4 Antibody Target Details MAP4 Application Details Handling References for anti-MAP4 Antibody (ABIN968326) back to top
Supplier Images
Western Blotting (WB) image for anti-MAP4 antibody (Microtubule-Associated Protein 4) (AA 583-702) (ABIN968326) Western blot analysis of MAP4 on HepG2 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3...
 image for anti-MAP4 antibody (Microtubule-Associated Protein 4) (AA 583-702) (ABIN968326) Human Endothelial
 image for anti-MAP4 antibody (Microtubule-Associated Protein 4) (AA 583-702) (ABIN968326) anti-Microtubule-Associated Protein 4 (MAP4) (AA 583-702) antibody (Image 3)