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|Application / Reactivity||Human|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (IF)||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||16 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||26 Antibodies|
|Antigen||Microtubule-Associated Protein 4 (MAP4) Antibodies|
|Epitope||AA 583-702 Alternatives|
|Conjugate||This MAP4 antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-MAP4 AntibodyTarget Details MAP4 Application Details Handling References for anti-MAP4 Antibody (ABIN968326) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human MAP4 aa. 583-702|
Target Details MAP4Product Details anti-MAP4 Antibody Application Details Handling References for anti-MAP4 Antibody (ABIN968326) Images back to top
|Alternative Name||MAP4 (MAP4 Antibody Abstract)|
|Background||The microtubule (MT) cytoskeleton functions in cytoplasmic organization, cellular movement, determination of cell polarity, intracellular transport, and chromosome segregation. Dynamic instability, continuous cycles of MT assembly (stabilization) and disassembly, mediates MT participation in these events. The function of MTs, particularly stabilization, is regulated by MT-associated proteins (MAPs). A subfamily of MAPs, called AP-MAPs (assembly promoting MAPs), includes tau, MAP2, and MAP4. These proteins are classified as type II MAPs and contain a C-terminal MT binding domain with 3 to 5 imperfect repeats of an 18 amino acid motif. While tau and MAP2 are specifically expressed in neuronal cells, MAP4 is the major MAP of nonneuronal mammalian cells. MAP activity and interaction with MTs are regulated by MARK (MAP/MT affinity-regulating kinase) and mapmodulin. MARK-mediated phosphorylation of MAPs on their homologous KXGS motifs results in detachment of MAPs from MTs and MT disruption. In addition, mapmodulin tightly interacts with the MT-binding domains of MAPs and hinders MAP binding to MTs. Thus, MAP4 is a highly regulated type II MAP that controls MT assembly and disassembly.|
|Molecular Weight||200-220 kDa|
|Pathways||p53 Signaling, Microtubule Dynamics|
Application DetailsProduct Details anti-MAP4 Antibody Target Details MAP4 Handling References for anti-MAP4 Antibody (ABIN968326) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-MAP4 Antibody Target Details MAP4 Application Details References for anti-MAP4 Antibody (ABIN968326) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Store undiluted at -20°C.|
References for anti-MAP4 Antibody (ABIN968326)Product Details anti-MAP4 Antibody Target Details MAP4 Application Details Handling Images back to top
|Product cited in:||
Drewes, Ebneth, Preuss, Mandelkow, Mandelkow: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." in: Cell, Vol. 89, Issue 2, pp. 297-308, 1997
Ulitzur, Humbert, Pfeffer: "Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5084-9, 1997
Chapin, Lue, Yu, Bulinski: "Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains." in: Biochemistry, Vol. 34, Issue 7, pp. 2289-301, 1995
ImagesProduct Details anti-MAP4 Antibody Target Details MAP4 Application Details Handling References for anti-MAP4 Antibody (ABIN968326) back to top