anti-Human MDM2 antibody for Immunoprecipitation

Recommended MDM2 Antibody (supplied by: Log in to see )

Antigen
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antibodies
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
Epitope
Center
46
42
36
29
26
24
15
15
15
12
9
8
7
7
6
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
378
135
95
14
8
6
4
2
2
2
2
2
2
1
1
1
Host
Rabbit
305
77
14
2
Clonality
Polyclonal
Conjugate
This MDM2 antibody is un-conjugated
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
262
136
108
83
71
40
38
24
12
12
12
12
4
4
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2854782
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN967511 BI IHC (f) IHC (fro) IP WB Mouse IgG1 AA 154-167 Log in to see SMP14 2
1 ABIN180331 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 154-167 Log in to see SMP14 3
1 ABIN2957662 IC IF IP IHC WB Rabbit Log in to see Polyclonal
1 ABIN214211 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN2469738 IP IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN2475548 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Log in to see SMP14 3
1 ABIN2706519 IC IF IP IHC WB Rabbit pSer166 Log in to see Polyclonal
1 ABIN1724157 IHC (fro) IHC (p) IP IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN1493454 IP Sepharose Rabbit AA 418-432 Log in to see Polyclonal
1 ABIN1495411 IP Magnetic Particles Rabbit AA 103-119 Log in to see Polyclonal
1 ABIN4904328 IF IHC IP WB Mouse IgG Log in to see
1 ABIN216012 IHC (fro) IF IHC (p) IP WB Mouse IgG2b, kappa AA 26-169 Log in to see
1 ABIN2312928 IC IF IP IHC WB Mouse IgG2b kappa AA 26-169 Log in to see 3G187
1 ABIN2625321 ICC IF IHC (p) IP WB Rabbit pSer166 Log in to see Polyclonal
1 ABIN2312952 IP IHC WB Mouse IgG1 AA 154-167 Log in to see 3H2649
1 ABIN5620135 ICC IF IHC IP WB Rabbit Center Log in to see Polyclonal
1 ABIN2313024 IP IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14
1 ABIN5583358 IHC (fro) IHC (p) IP Mouse IgG1 Log in to see SMP14
1 ABIN643838 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 Log in to see
1 ABIN2767770 IHC (fro) IP IHC (p) IHC WB Mouse IgG1 AA 154-167 Log in to see SMP14

General

Antigen Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antibodies
Epitope Center
(46), (42), (36), (29), (26), (24), (15), (15), (15), (12), (9), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(378), (135), (95), (14), (8), (6), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(305), (77), (14), (2)
Clonality
Polyclonal
Conjugate This MDM2 antibody is un-conjugated
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(262), (136), (108), (83), (71), (40), (38), (24), (12), (12), (12), (12), (4), (4), (2), (1), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-MDM2 Antibody

Target Details MDM2 Application Details Handling Images
Cross-Reactivity Cat (Feline), Dog (Canine), Pig (Porcine)
Cross-Reactivity (Details) Cat (95 %), Dog (91 %), Pig (93 %)
Characteristics Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)
MDM2 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.
Isotype IgG

Target Details MDM2

Product Details anti-MDM2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MDM2 (MDM2 Antibody Abstract)
Background This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Cellular Localization: Nucleus , nucleoplasm , Cytoplasm , nucleolus
Molecular Weight 55 kDa
Gene ID 4193
Research Area Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Autophagy

Application Details

Product Details anti-MDM2 Antibody Target Details MDM2 Handling Images back to top
Application Notes Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:1000*
Comment

Positive Control: H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T

Restrictions For Research Use only

Handling

Product Details anti-MDM2 Antibody Target Details MDM2 Application Details Images back to top
Format Liquid
Concentration 0.74 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-MDM2 Antibody Target Details MDM2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg 293T w...
Immunohistochemistry (IHC) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, usin...
Immunofluorescence (IF) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MDM2, ...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg Neuro2...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg H1299 ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ChIP Image Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of ei...
Immunoprecipitation (IP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IP Image MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples:...