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anti-Human p300 antibody for Chromatin Immunoprecipitation

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Reactivity
Human
221
72
60
14
3
2
2
2
2
Host
Mouse
156
70
Clonality
Monoclonal
Conjugate
This p300 antibody is un-conjugated
10
10
8
6
6
6
2
2
2
2
2
2
2
2
2
2
2
2
Application
ChIP DNA-Sequencing (ChIPSeq), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
144
94
64
57
42
41
37
24
12
5
4
2
1
1
1
1
Supplier
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Catalog No. ABIN2668569
$ 478.33
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008906772 ABIN152047 ChIP ICC IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW105 3
0.0008906772 ABIN152048 ChIP ICC IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW109 3
0.0008906772 ABIN152981 ChIP ICC IF IHC IHC (p) IP WB Rabbit AA 1000-1050 Log in to see Polyclonal
0.0008906772 ABIN1978817 ChIP IF IP IHC (p) PLA WB Rabbit IgG AA 950-1000 Log in to see Polyclonal
0.0008906772 ABIN2667672 ChIPSeq ChIP ICC IF IP WB Mouse IgG1 Log in to see NM11

Top referenced anti-p300 antibody for Chromatin Immunoprecipitation

Similar anti-p300 Antibodies

Application / Reactivity Human
ChIP DNA-Sequencing (ChIPSeq) 2 Antibodies
Chromatin Immunoprecipitation (ChIP) 6 Antibodies
Dot Blot (DB) 57 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 91 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 53 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 65 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 49 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 154 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Reactivity Human
(221), (72), (60), (14), (3), (2), (2), (2), (2)
Host Mouse
(156), (70)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p300 antibody is un-conjugated
(10), (10), (8), (6), (6), (6), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application ChIP DNA-Sequencing (ChIPSeq), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(144), (94), (64), (57), (42), (41), (37), (24), (12), (5), (4), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN2668569) Images
Purification Protein A Chromatography
Immunogen The antibody was raised against full-length p300 purified from human 293 cells.
Clone NM11
Isotype IgG1

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN2668569) Images back to top
Antigen
Alternative Name p300 (EP300 Antibody Abstract)
Background P300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.
Molecular Weight 300 kDa
Gene ID 2033
Research Area Transcription Factors
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN2668569) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN2668569) Images back to top
Concentration 1 μg/μL
Buffer Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date 6 months

References for anti-p300 Antibody (ABIN2668569)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Product cited in:

Dornan, Eckert, Wallace, Shimizu, Ramsay, Hupp, Ball: "Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 10083-98, 2004

Bernat, Avvakumov, Mymryk, Banks: "Interaction between the HPV E7 oncoprotein and the transcriptional coactivator p300." in: Oncogene, Vol. 22, Issue 39, pp. 7871-81, 2003

Dallas, Yaciuk, Moran: "Characterization of monoclonal antibodies raised against p300: both p300 and CBP are present in intracellular TBP complexes." in: Journal of virology, Vol. 71, Issue 2, pp. 1726-31, 1997

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN2668569) back to top
Supplier Images
ChIP DNA-Sequencing (ChIPSeq) image for anti-p300 antibody (E1A Binding Protein P300) (ABIN2668569) p300 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Se...
Chromatin Immunoprecipitation (ChIP) image for anti-p300 antibody (E1A Binding Protein P300) (ABIN2668569) p300 antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT® High Sensit...
Immunofluorescence (IF) image for anti-p300 antibody (E1A Binding Protein P300) (ABIN2668569) p300 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stain...