|Antigen||E1A Binding Protein P300 (EP300) Antibodies|
|Conjugate||This p300 antibody is un-conjugated Alternatives|
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-p300 AntibodyTarget Details p300 Application Details Handling Images
|Purification||Prepared from tissue culture supernatant.|
|Immunogen||p300 peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).|
Target Details p300Product Details anti-p300 Antibody Application Details Handling Images back to top
|Alternative Name||EP300 / P300 (EP300 Antibody Abstract)|
|Background||Synonyms: E1A binding protein p300, E1A-associated protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT|
|Research Area||Transcription Factors|
|Pathways||p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size|
Application DetailsProduct Details anti-p300 Antibody Target Details p300 Handling Images back to top
Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against p300 at a dilution of 1: 10 to 1: 50 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Mouse IgG [H&L] (Goat). Anti-p300 is suitable for thedetection by immunoblot of human p300. Partial reaction was observed against the CREBbinding Proterin (CBP). This product was also tested by immunoporecipitation and found to be reactive using 50 mLper assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-p300 Antibody Target Details p300 Application Details Images back to top
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store original vial at 2-8 °C. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.|