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anti-Human p300 antibody for Electrophoretic Mobility-Shift Assay

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Epitope
N-Term
54
46
36
17
15
12
9
7
6
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
255
104
92
31
30
14
3
2
2
2
2
Host
Rabbit
158
103
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
10
10
10
8
6
6
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
176
95
73
67
67
57
45
43
24
16
4
4
2
1
1
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Catalog No. ABIN116526
$ 500.50
Plus shipping costs $45.00

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General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope N-Term
(54), (46), (36), (17), (15), (12), (9), (7), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(255), (104), (92), (31), (30), (14), (3), (2), (2), (2), (2)
Host Rabbit
(158), (103)
Clonality
Polyclonal
Conjugate This p300 antibody is un-conjugated
(10), (10), (10), (8), (6), (6), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(176), (95), (73), (67), (67), (57), (45), (43), (24), (16), (4), (4), (2), (1), (1)
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling Images
Purification Prepared from tissue culture supernatant.
Immunogen p300 peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Target Details p300

Product Details anti-p300 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EP300 / P300 (EP300 Antibody Abstract)
Background Synonyms: E1A binding protein p300, E1A-associated protein p300, Histone acetyltransferase p300, KAT3B, p300 HAT
Gene ID 2033
NCBI Accession NP_001420
UniProt Q09472
Research Area Transcription Factors
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling Images back to top
Application Notes Suitable for Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against p300 at a dilution of 1: 10 to 1: 50 followed by reaction with Peroxidaseconjugated Affinity Purified anti-Mouse IgG [H&L] (Goat). Anti-p300 is suitable for thedetection by immunoblot of human p300. Partial reaction was observed against the CREBbinding Proterin (CBP). This product was also tested by immunoporecipitation and found to be reactive using 50 mLper assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details Images back to top
Format Liquid
Concentration 50.0 µg/mL
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store original vial at 2-8 °C. After reconstitution store the antibody undiluted at 2-8 °C for up to one month or (in aliquots) at -20 °C for longer.