anti-Human p300 antibody for Western Blotting

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • E1A-associated protein p300
  • EP300
  • ep300
  • Ep300
Epitope
AA 1572-2371
56
52
37
18
15
11
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
265
126
115
38
36
15
3
3
3
3
3
Host
Mouse
161
111
Clonality
Monoclonal
Conjugate
This p300 antibody is un-conjugated
12
12
10
8
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
185
96
83
71
54
51
49
48
28
10
5
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN151948
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN152047 ChIP EM FACS ICC IF IP in vitro WB Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105 8
3.3487515 ABIN152048 ChIP ICC IF IP WB Mouse IgG1 kappa AA 1572-2371, AA 2107-2283 Log in to see RW109 4
3.3487515 ABIN967440 IP WB Mouse IgG2b Log in to see NM11 3
3.3487515 ABIN152981 ChIP ICC IF IHC IHC (p) IP WB Rabbit AA 1000-1050 Log in to see Polyclonal 4
3.3487515 ABIN3042372 IHC (p) WB Rabbit IgG AA 2065-2414 Log in to see Polyclonal
3.3487515 ABIN782395 EIA IF IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1D2
3.3487515 ABIN969098 ELISA WB Mouse IgG1 Log in to see 7D8A6 2
3.3487515 ABIN152980 IHC IP PLA WB Rabbit Log in to see Polyclonal 1
3.3487515 ABIN1532373 ELISA WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN1533367 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN3186252 ELISA IHC WB Rabbit IgG Ser342 Log in to see Polyclonal
3.3487515 ABIN3186253 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal
3.3487515 ABIN2957697 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN2707672 IHC WB Rabbit N-Term Log in to see Polyclonal
3.3487515 ABIN2705082 WB Rabbit C-Term, pSer1834 Log in to see Polyclonal
3.3487515 ABIN2801289 WB Rabbit acLys1542 Log in to see Polyclonal
3.3487515 ABIN2801290 WB Rabbit acLys1558, acLys1560 Log in to see Polyclonal
3.3487515 ABIN2957698 IHC WB Rabbit Log in to see Polyclonal
3.3487515 ABIN2957699 WB Rabbit Log in to see Polyclonal
3.3487515 ABIN498122 IHC (p) WB Rabbit Log in to see Polyclonal

Top referenced anti-p300 antibody for Western Blotting

Similar anti-p300 Antibodies

Application / Reactivity Rat (Rattus) Primate
Chromatin Immunoprecipitation (ChIP) 5 Antibodies 5 Antibodies
Electron Microscopy (EM) 1 Antibodies 1 Antibodies
ELISA 11 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 42 Antibodies 36 Antibodies
Immunofluorescence (IF) 51 Antibodies 36 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 28 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies 1 Antibodies
Immunoprecipitation (IP) 27 Antibodies
In vitro Assay (in vitro) 1 Antibodies
Western Blotting (WB) 70 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope AA 1572-2371
(56), (52), (37), (18), (15), (11), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
(265), (126), (115), (38), (36), (15), (3), (3), (3), (3), (3)
Host Mouse
(161), (111)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p300 antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(185), (96), (83), (71), (54), (51), (49), (48), (28), (10), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling ProductDetails: References for anti-p300 antibody (ABIN151948) Images
Purification Protein A purified
Immunogen Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Clone RW128
Isotype IgG1 kappa

Target Details p300

Product Details anti-p300 Antibody Application Details Handling ProductDetails: References for anti-p300 antibody (ABIN151948) Images back to top
Antigen
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Molecular Weight Theoretical MW: 300 kDa
Gene ID 2033
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling ProductDetails: References for anti-p300 antibody (ABIN151948) Images back to top
Application Notes Western Blot 1:250-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500, Flow (Intracellular) 1 μg/mLThis KAT3B/p300 (RW128) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band is seen at ~300 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details ProductDetails: References for anti-p300 antibody (ABIN151948) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

ProductDetails: References for anti-p300 antibody (ABIN151948)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Product cited in:

Nemethova, Wintersberger: "Polyomavirus large T antigen binds the transcriptional coactivator protein p300." in: Journal of virology, Vol. 73, Issue 2, pp. 1734-9, 1999

Bhattacharya, Michels, Leung, Arany, Kung, Livingston: "Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1." in: Genes & development, Vol. 13, Issue 1, pp. 64-75, 1999

Eckner, Ludlow, Lill, Oldread, Arany, Modjtahedi, DeCaprio, Livingston, Morgan: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (Sample species: Mouse (Murine)). Further details: Immunoprecipitation

Eckner, Yao, Oldread, Livingston: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996

Eckner, Ewen, Newsome, Gerdes, DeCaprio, Lawrence, Livingston: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling ProductDetails: References for anti-p300 antibody (ABIN151948) back to top
Supplier Images
Western Blotting (WB) image for anti-p300 antibody (E1A Binding Protein P300) (AA 1572-2371) (ABIN151948) Detection of p300 in a HeLa nuclear extract using ABIN151948 (1:250). ECL: 15 second...