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anti-Mustelid p300 antibody for Immunofluorescence

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Epitope
AA 1572-2371, AA 1921-2023
54
54
36
18
18
12
9
7
6
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
265
112
100
39
38
14
3
2
2
2
2
Host
Mouse
160
111
Clonality
Monoclonal
Conjugate
This p300 antibody is un-conjugated
12
12
10
8
6
6
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), In vitro Assay (in vitro), Western Blotting (WB)
186
95
81
77
67
57
51
45
24
17
5
4
2
2
1
1
Supplier
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Catalog No. ABIN152047
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN152048 ChIP ICC IF IP WB Mouse IgG1 kappa AA 1572-2371, AA 2107-2283 Log in to see RW109 4
0.0010840179 ABIN407278 ICC IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW128 5
0.0010840179 ABIN407352 ICC IF IP WB DyLight 488 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN407350 ICC IF IP WB Biotin Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN407353 ICC IF IP WB HRP Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN407275 ICC IF IP WB DyLight 488 Mouse IgG1 kappa AA 1572-2371 Log in to see RW128
0.0010840179 ABIN407273 ICC IF IP WB Biotin Mouse IgG1 kappa AA 1572-2371 Log in to see RW128
0.0010840179 ABIN4328389 ICC IF IP WB DyLight 550 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328392 ICC IF IP WB DyLight 650 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328397 ChIP ICC IF IP WB DyLight 405 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328399 ChIP ICC IF IP WB DyLight 350 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328401 ChIP ICC IF IP WB PE Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328403 ChIP ICC IF IP WB PerCP Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328405 ChIP ICC IF IP WB Allophycocyanin Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328407 ChIP ICC IF IP WB DyLight 755 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328409 ChIP ICC IF IP WB DyLight 680 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328411 ChIP ICC IF IP WB DyLight 405LS Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328416 ChIP ICC IF IP WB Alexa Fluor 405 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328417 ChIP ICC IF IP WB Alexa Fluor 488 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0010840179 ABIN4328418 ChIP ICC IF IP WB Alexa Fluor 647 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105

Top referenced anti-p300 antibody for Immunofluorescence

Similar anti-p300 Antibodies

Application / Reactivity Rat (Rattus) Primate Mustelid
Chromatin Immunoprecipitation (ChIP) 15 Antibodies 15 Antibodies 15 Antibodies
ELISA 8 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 44 Antibodies 38 Antibodies 37 Antibodies
Immunofluorescence (IF) 52 Antibodies 38 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 54 Antibodies 40 Antibodies
Western Blotting (WB) 64 Antibodies 40 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope AA 1572-2371, AA 1921-2023
(54), (54), (36), (18), (18), (12), (9), (7), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus)
(265), (112), (100), (39), (38), (14), (3), (2), (2), (2), (2)
Host Mouse
(160), (111)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p300 antibody is un-conjugated
(12), (12), (10), (8), (6), (6), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Electron Microscopy (EM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), In vitro Assay (in vitro), Western Blotting (WB)
(186), (95), (81), (77), (67), (57), (51), (45), (24), (17), (5), (4), (2), (2), (1), (1)
Pubmed 8 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN152047) Images
Specificity This is specific for p300 protein. This recognizes residues 1921-2023.
Purification Protein G purified
Immunogen Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Clone RW105
Isotype IgG1 kappa

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN152047) Images back to top
Antigen
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background Gene Symbol: EP300
Molecular Weight Theoretical MW: 300 kDa
Gene ID 2033, 328572, 170915
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN152047) Images back to top
Application Notes Western Blot 1:250-1:500, Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, In vitro assay, Electron Microscopy
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN152047) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, pH 7.4
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-p300 Antibody (ABIN152047)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Product cited in:

Yi, Wang, Feng, Pintilie, Foulds, Lanz, Ludtke, Schmid, Chiu, OMalley: "Structure of a biologically active estrogen receptor-coactivator complex on DNA." in: Molecular cell, Vol. 57, Issue 6, pp. 1047-58, 2015 Further details: In vitro Assay,Immunoelectron Microscopy

He, Zheng, Zhang, Zhang, Wang, Yang, Sun, Wu, Wen: "KLF4 mediates the link between TGF-β1-induced gene transcription and H3 acetylation in vascular smooth muscle cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 29, Issue 9, pp. 4059-70, 2015

Byun, Chang, Chung, Moss, Kim: "Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter." in: Cancer letters, Vol. 326, Issue 1, pp. 96-104, 2012 (Sample species: Human). Further details: Chromatin Immunoprecipitation

Nemethova, Wintersberger: "Polyomavirus large T antigen binds the transcriptional coactivator protein p300." in: Journal of virology, Vol. 73, Issue 2, pp. 1734-9, 1999 (Sample species: Mouse (Murine)). Further details: Western Blotting,Immunoprecipitation

Bhattacharya, Michels, Leung, Arany, Kung, Livingston: "Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1." in: Genes & development, Vol. 13, Issue 1, pp. 64-75, 1999

Eckner, Ludlow, Lill, Oldread, Arany, Modjtahedi, DeCaprio, Livingston, Morgan: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (Sample species: Mouse (Murine)). Further details: Immunoprecipitation

Eckner, Yao, Oldread, Livingston: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996

Eckner, Ewen, Newsome, Gerdes, DeCaprio, Lawrence, Livingston: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994 (Sample species: Human). Further details: Western Blotting

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN152047) back to top
Supplier Images
Western Blotting (WB) image for anti-p300 antibody (E1A Binding Protein P300) (AA 1572-2371) (ABIN152047) p300 detected in a HeLa nuclear extract using ABIN152047 (1:250). ECL: 20 minute exp...