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anti-Mustelid p300 antibody for Western Blotting

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • A430090G16
  • A730011L11
  • KAT3B
  • p300
  • p300 HAT
  • RSTS2
Epitope
AA 1572-2371
54
46
36
18
15
12
9
7
6
5
4
4
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus), Mustelid
256
104
92
31
30
14
3
2
2
2
2
Host
Mouse
159
103
Clonality
Monoclonal
Conjugate
This p300 antibody is un-conjugated
10
10
10
8
6
6
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
177
95
73
68
67
57
45
43
24
16
5
4
2
1
1
1
Supplier
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Available images

Catalog No. ABIN152047
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN407278 ICC IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW128 3
0.13476625 ABIN152048 ChIP ICC IF IP WB Mouse IgG1 kappa AA 1572-2371 Log in to see RW109 3
0.0011760078 ABIN4328389 ICC IF IP WB DyLight 550 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328392 ICC IF IP WB DyLight 650 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328395 ChIP IP WB FITC Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328397 ChIP ICC IF IP WB DyLight 405 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328399 ChIP ICC IF IP WB DyLight 350 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328401 ChIP ICC IF IP WB PE Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328403 ChIP ICC IF IP WB PerCP Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328405 ChIP ICC IF IP WB Allophycocyanin Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328407 ChIP ICC IF IP WB DyLight 755 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328409 ChIP ICC IF IP WB DyLight 680 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328411 ChIP ICC IF IP WB DyLight 405LS Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328416 ChIP ICC IF IP WB Alexa Fluor 405 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328417 ChIP ICC IF IP WB Alexa Fluor 488 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328418 ChIP ICC IF IP WB Alexa Fluor 647 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4328419 ChIP ICC IF IP WB Alexa Fluor 700 Mouse IgG1 kappa AA 1572-2371, AA 1921-2023 Log in to see RW105
0.0011760078 ABIN4228146 IP WB FITC Mouse IgG1 kappa AA 1572-2371 Log in to see RW128
0.0011760078 ABIN4328390 ICC IF IP WB DyLight 650 Mouse IgG1 kappa AA 1572-2371 Log in to see RW128
0.0011760078 ABIN4328391 ICC IF IP WB DyLight 550 Mouse IgG1 kappa AA 1572-2371 Log in to see RW128

Top referenced anti-p300 antibody for Western Blotting

Similar anti-p300 Antibodies

Application / Reactivity Rat (Rattus) Mustelid Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 15 Antibodies 15 Antibodies 15 Antibodies
ELISA 8 Antibodies 11 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 38 Antibodies 31 Antibodies
Immunofluorescence (IF) 46 Antibodies 31 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 48 Antibodies 33 Antibodies
Western Blotting (WB) 58 Antibodies 33 Antibodies

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope AA 1572-2371
(54), (46), (36), (18), (15), (12), (9), (7), (6), (5), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus), Mustelid
(256), (104), (92), (31), (30), (14), (3), (2), (2), (2), (2)
Host Mouse
(159), (103)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p300 antibody is un-conjugated
(10), (10), (10), (8), (6), (6), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(177), (95), (73), (68), (67), (57), (45), (43), (24), (16), (5), (4), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN152047) Images
Specificity This is specific for p300 protein. This recognizes residues 1921-2023.
Purification Protein G purified
Immunogen Fusion protein containing residues 1572-2371 of human KAT3B/p300.
Clone RW105
Isotype IgG1 kappa

Target Details p300

Product Details anti-p300 Antibody Application Details Handling References for anti-p300 Antibody (ABIN152047) Images back to top
Antigen
Alternative Name KAT3B/p300 (EP300 Antibody Abstract)
Background P300 is a relatively stable, ubiquitously expressed, nuclear phosphoprotein which isconserved among a variety of mammalian species. It is actively synthesized andphosphorylated in both quiescent and proliferating cells.Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) andp300 are closely related transcriptional coactivators and have been shown to directlyinteract with many different DNA-binding transcription factors including nuclear hormonereceptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Both CBP andp300 have been shown to display histone acetyltransferase (HAT) activity, capable ofacetylating all four core histone particles in nucleosomes. As a result of HAT activity, ithas been suggested CBP and p300 may play a direct role in activating chromatin fortranscription. Alternate Names: anti-CREBBP/EP300 inhibitory protein 1 antibody, anti-Cyclic AMP responsive enhancerbinding protein antibody, anti-EP300: E1A binding protein p300 antibody, anti-RB andP300 binding protein EID 1 antibody, anti-Retinoblastoma protein associated proteinantibody.
Gene Symbol: EP300
Gene ID 2033, 328572, 170915
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 Antibody (ABIN152047) Images back to top
Application Notes This KAT3B/p300 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. In Western blot a band is seen at ~300 kDa, representing the p300 protein. Lower non-specific MW bands (~45-66 kDa) ay be seen with longer exposure times.
Recommended dilutions: Chromatin Immunoprecipitation, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Western Blot 1:250-1:500
Protocol Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 Antibody (ABIN152047) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, pH 7.4, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.

References for anti-p300 Antibody (ABIN152047)

Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
Background publications

Eckner, Ludlow, Lill, Oldread, Arany, Modjtahedi, DeCaprio, Livingston, Morgan: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996

Eckner, Yao, Oldread, Livingston: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996

Eckner, Ewen, Newsome, Gerdes, DeCaprio, Lawrence, Livingston: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 Antibody (ABIN152047) back to top
Supplier Images
Western Blotting (WB) image for anti-p300 antibody (E1A Binding Protein P300) (AA 1572-2371) (ABIN152047) p300 detected in a HeLa nuclear extract using ABIN152047 (1:250). ECL: 20 minute exp...