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|Antigen||E1A Binding Protein P300 (EP300) Antibodies|
|Epitope||AA 1572-2371 Alternatives|
|Reactivity||Human, Mouse (Murine), Mustelid, Primate, Rat (Rattus) Alternatives|
|Conjugate||This p300 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-p300 AntibodyTarget Details p300 Application Details Handling References for anti-p300 antibody (ABIN151948) Images
|Purification||Protein A purified|
|Immunogen||Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]|
Target Details p300Product Details anti-p300 Antibody Application Details Handling References for anti-p300 antibody (ABIN151948) Images back to top
|Alternative Name||KAT3B/p300 (EP300 Antibody Abstract)|
|Background||Gene Symbol: EP300|
|Molecular Weight||Theoretical MW: 300 kDa|
|Pathways||p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size|
Application DetailsProduct Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 antibody (ABIN151948) Images back to top
|Application Notes||Western Blot 1:250-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunoprecipitation 1:10-1:500, Flow (Intracellular) 1 μg/mLThis KAT3B/p300 (RW128) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot, where a band is seen at ~300 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for KAT3B / p300 Antibody Western Blot Protocol
1. Perform SDS-PAGE (3-8 % Tris-acetate) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk in TBS for 1.5 hours.
. Dilute the mouse anti-p300 primary antibody in blocking buffer and incubate overnight at 4 °C.
. Wash the membrane in water for 5 minutes and apply the diluted mouse-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBS containing 0.05-0.1 % Tween-20 for 10-20 minutes.
. Wash the blot in type I water for an additional 10-20 minutes (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (Amersham's ECL is the standard reagent used).Note: Tween-20 can be added to the blocking buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
|Restrictions||For Research Use only|
HandlingProduct Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 antibody (ABIN151948) Images back to top
PBS ( pH 7.4)
Buffer contains: 0.1 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-p300 antibody (ABIN151948)Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top
|Product cited in:||
Nemethova, Wintersberger: "Polyomavirus large T antigen binds the transcriptional coactivator protein p300." in: Journal of virology, Vol. 73, Issue 2, pp. 1734-9, 1999
Bhattacharya, Michels, Leung, Arany, Kung, Livingston: "Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1." in: Genes & development, Vol. 13, Issue 1, pp. 64-75, 1999
Eckner, Ludlow, Lill, Oldread, Arany, Modjtahedi, DeCaprio, Livingston, Morgan: "Association of p300 and CBP with simian virus 40 large T antigen." in: Molecular and cellular biology, Vol. 16, Issue 7, pp. 3454-64, 1996 (Sample species: Mouse (Murine)). Further details: Immunoprecipitation
Eckner, Yao, Oldread, Livingston: "Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation." in: Genes & development, Vol. 10, Issue 19, pp. 2478-90, 1996
Eckner, Ewen, Newsome, Gerdes, DeCaprio, Lawrence, Livingston: "Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor." in: Genes & development, Vol. 8, Issue 8, pp. 869-84, 1994
ImagesProduct Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 antibody (ABIN151948) back to top