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anti-Cow (Bovine) p53 antibody for Immunocytochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 20-25
156
103
86
67
66
65
56
52
52
52
47
46
42
40
31
30
28
25
25
22
22
21
20
17
17
14
14
14
14
14
12
11
11
10
10
9
9
8
8
8
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Primate
2086
394
328
120
100
88
56
54
29
23
19
15
14
10
10
5
5
4
4
3
3
3
3
2
2
Host
Mouse
1254
930
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
68
61
49
45
31
31
30
23
23
17
17
16
16
14
14
14
14
14
14
13
13
13
13
13
12
12
6
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1658
732
564
491
441
420
396
229
193
170
53
38
35
17
16
12
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4342817
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010101626 ABIN4342818 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0010101626 ABIN4342820 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342821 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342819 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0010101626 ABIN4342822 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342835 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342836 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342837 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342838 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342841 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342842 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342843 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342839 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342845 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342846 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342847 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PE Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342848 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342850 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0010101626 ABIN4342849 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG2b kappa AA 37-45 Log in to see SPM590

Similar anti-p53 Antibodies

Application / Reactivity Primate Human
ELISA 41 Antibodies 681 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 17 Antibodies
Flow Cytometry (FACS) 52 Antibodies 381 Antibodies
Immunocytochemistry (ICC) 52 Antibodies
Immunofluorescence (IF) 54 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 53 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 50 Antibodies
Simple Western (SimWes) 3 Antibodies
Western Blotting (WB) 85 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 20-25
(156), (103), (86), (67), (66), (65), (56), (52), (52), (52), (47), (46), (42), (40), (31), (30), (28), (25), (25), (22), (22), (21), (20), (17), (17), (14), (14), (14), (14), (14), (12), (11), (11), (10), (10), (9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Primate
(2086), (394), (328), (120), (100), (88), (56), (54), (29), (23), (19), (15), (14), (10), (10), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Mouse
(1254), (930), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(68), (61), (49), (45), (31), (31), (30), (23), (23), (17), (17), (16), (16), (14), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (12), (12), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1658), (732), (564), (491), (441), (420), (396), (229), (193), (170), (53), (38), (35), (17), (16), (12), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Specificity Recognizes a 53 kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Purification Protein A purified
Immunogen Recombinant human wild-type p53 protein (BP53-12) & Recombinant human wild type p53 protein expressed in E. coli (DO-7)
Clone BP53-12-DO-7
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
Molecular Weight Theoretical MW: 53 kDa
UniProt P04637
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Western Blot 0.5-1.0 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 μg/mL, Immunoprecipitation 0.5-1 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Buffer PBS with 0.05 % BSA.
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.