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|Application / Reactivity||Sheep (Ovine)||Human|
|Enzyme Immunoassay (EIA)||2 Antibodies||17 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||220 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||2 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||2 Antibodies|
|Affinity Purification (AP)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Chromatin Immunoprecipitation (ChIP)||10 Antibodies|
|Dot Blot (DB)||31 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Gel Shift (GS)||3 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||35 Antibodies|
|Immunocytochemistry (ICC)||78 Antibodies|
|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Epitope||AA 20-25 Alternatives|
|Reactivity||Cat (Feline), Cow (Bovine), Horse (Equine), Human, Sheep (Ovine) Alternatives|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|3 references available|
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Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling References for anti-p53 Antibody (ABIN120054) Images
|Specificity||This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Horse, Bovine, Cat and Sheep.|
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.|
Target Details p53Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN120054) Images back to top
|Alternative Name||p53 (TP53) (TP53 Antibody Abstract)|
|Background||Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling|
Application DetailsProduct Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN120054) Images back to top
Flow Cyometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/25): Heat induced epitope retrieval with 0.01 M citrate buffer, pH 6.0 is recommended. Positive Control Tissue: Colon or breast carcinoma. ELISA. Western Blotting (1/1000). Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN120054) Images back to top
|Buffer||PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-p53 Antibody (ABIN120054)Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Sironi, Riccaboni, Mertel et al.: "p53 protein expression in conjunctival squamous cell carcinomas of domestic animals." in: Veterinary ophthalmology, Vol. 2, Issue 4, pp. 227-231, 2001 (PubMed).
Levesque, DCosta, Angelopoulou et al.: "Time-resolved immunofluorometric assay of p53 protein." in: Clinical chemistry, Vol. 41, Issue 12 Pt 1, pp. 1720-9, 1996 (PubMed).
Vojt?sek, Bártek, Midgley et al.: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992 (PubMed).