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anti-Hamster p53 antibody for Immunohistochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 370-378, C-Term
153
103
87
69
67
66
57
56
53
53
47
46
43
42
31
30
30
25
24
24
23
21
21
20
17
17
15
15
15
15
15
12
11
11
10
10
9
8
8
8
8
7
7
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
2160
436
334
120
101
89
56
54
29
19
18
16
15
10
10
5
4
4
4
4
3
3
3
2
2
Host
Mouse
1304
959
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
71
66
53
47
34
33
23
23
19
19
19
17
16
16
16
16
16
16
15
15
15
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
4
3
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
1692
733
637
545
531
463
388
281
253
179
55
35
34
18
17
15
13
13
12
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN3026354
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN3026355 FACS IF IHC WB Mouse IgG2b, kappa AA 370-378, C-Term Log in to see PAb 122
1 ABIN201678 FACS IP IHC WB Mouse IgG2b AA 371-380 Log in to see

Similar anti-p53 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Hamster Monkey
Cytometry by Time of Flight (CyTOF) 18 Antibodies 6 Antibodies 4 Antibodies 4 Antibodies 4 Antibodies 2 Antibodies
Simple Western (SimWes) 9 Antibodies 4 Antibodies 4 Antibodies 3 Antibodies 3 Antibodies
Western Blotting (WB) 1605 Antibodies 317 Antibodies 231 Antibodies 49 Antibodies 48 Antibodies 102 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 55 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 370-378, C-Term
(153), (103), (87), (69), (67), (66), (57), (56), (53), (53), (47), (46), (43), (42), (31), (30), (30), (25), (24), (24), (23), (21), (21), (20), (17), (17), (15), (15), (15), (15), (15), (12), (11), (11), (10), (10), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Hamster, Human, Monkey, Mouse (Murine), Rat (Rattus)
(2160), (436), (334), (120), (101), (89), (56), (54), (29), (19), (18), (16), (15), (10), (10), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2)
Host Mouse
(1304), (959), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(71), (66), (53), (47), (34), (33), (23), (23), (19), (19), (19), (17), (16), (16), (16), (16), (16), (16), (15), (15), (15), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(1692), (733), (637), (545), (531), (463), (388), (281), (253), (179), (55), (35), (34), (18), (17), (15), (13), (13), (12), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Characteristics This antibody is specific to a conserved determinant of the p53 protein. It has been used successfully to immunoprecipitate p53 oncoprotein complexes with simian virus 40 (SV40) large T antigen. Reportedly, the p53 antibody precipitated the most ATPase-active form of T antigen. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. When DNA damage occurs, it can activate repair proteins, arrest growth by holding the cell cycle at the G1/S regulation point, thereby increasing time available for repair, and if the damage is not repairable, initiate apoptosis.
Mutations involving p53 are found in a wide variety of malignant tumors including breast, ovary, bladder, colon, lung, and melanoma. The substitution of an arginine for a proline residue at position 72 may result in cancer susceptibility, but this has not be conclusively determined.
Purification Protein G affinity chromatography
Immunogen SV40-transformed mouse B4 cells were used as the immunogen for this antibody. Its epitope maps near the C-terminus end (aa 370-378) of p53.
Clone PAb 122
Isotype IgG2b, kappa

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background This antibody is specific to a conserved determinant of the p53 protein. It has been used successfully to immunoprecipitate p53 oncoprotein complexes with simian virus 40 (SV40) large T antigen. Reportedly, the p53 antibody precipitated the most ATPase-active form of T antigen. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. When DNA damage occurs, it can activate repair proteins, arrest growth by holding the cell cycle at the G1/S regulation point, thereby increasing time available for repair, and if the damage is not repairable, initiate apoptosis.
Mutations involving p53 are found in a wide variety of malignant tumors including breast, ovary, bladder, colon, lung, and melanoma. The substitution of an arginine for a proline residue at position 72 may result in cancer susceptibility, but this has not be conclusively determined.
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (Frozen only): 0.5-1.0 μg/mL for 30 minutes at RT
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the p53 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).