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anti-Human p53 antibody for Chromatin Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
Center
141
87
63
54
51
50
49
48
47
46
43
40
39
30
27
25
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
8
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1783
320
269
100
34
21
19
13
11
11
10
10
9
5
4
4
4
3
3
3
2
2
1
Host
Rabbit
1234
654
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
61
51
39
33
23
23
17
17
14
14
14
14
14
14
13
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1418
601
529
318
314
306
235
156
101
86
53
39
35
17
16
12
11
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2855525
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008842582 ABIN2779319 ChIP ELISA WB Rabbit N-Term Log in to see Polyclonal 1
0.0008842582 ABIN2779318 ChIP IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.0008842582 ABIN2779317 ChIP IHC WB Rabbit N-Term Log in to see Polyclonal 1
0.0008842582 ABIN2779330 ChIP ELISA WB Rabbit N-Term Log in to see Polyclonal 1
0.0008842582 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see PAb 1801 5
0.0008842582 ABIN2779171 ChIP WB Mouse IgG Log in to see 1
0.0008842582 ABIN2779172 ChIP WB Mouse IgG Log in to see 1
0.0008842582 ABIN152309 ChIP FACS ICC IF IHC IHC (p) IP PLA WB Rabbit IgG AA 50-100 Log in to see Polyclonal
0.0008842582 ABIN1721640 ChIP ELISA FACS ICC IHC IP WB Mouse IgG1 Log in to see PAb1801
0.0008842582 ABIN1982255 ChIP IP WB Sheep IgG AA 7-393 Log in to see Polyclonal

Top referenced anti-p53 antibody for Chromatin Immunoprecipitation

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Application / Reactivity Human
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Dot Blot (DB) 31 Antibodies
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ELISA (Capture) 2 Antibodies
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Flow Cytometry (FACS) 220 Antibodies
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Immunoassay (IA) 1 Antibodies
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Immunocytochemistry (ICC) 82 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 311 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 515 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Center
(141), (87), (63), (54), (51), (50), (49), (48), (47), (46), (43), (40), (39), (30), (27), (25), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1783), (320), (269), (100), (34), (21), (19), (13), (11), (11), (10), (10), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (1)
Host Rabbit
(1234), (654), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(61), (51), (39), (33), (23), (23), (17), (17), (14), (14), (14), (14), (14), (14), (13), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1418), (601), (529), (318), (314), (306), (235), (156), (101), (86), (53), (39), (35), (17), (16), (12), (11), (11), (11), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Characteristics Rabbit Polyclonal antibody to p53 (tumor protein p53)
p53 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Cellular Localization: Cytoplasm , Nucleus , Endoplasmic reticulum
Molecular Weight 44 kDa
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , HCT116 24hr cisplatin 30uM , *HUVEC

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-p53 antibody (Tumor Protein P53) (Center) (ABIN2855525) IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ...
Immunofluorescence (IF) image for anti-p53 antibody (Tumor Protein P53) (Center) (ABIN2855525) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (Center) (ABIN2855525) WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted ...
Immunoprecipitation (IP) image for anti-p53 antibody (Tumor Protein P53) (Center) (ABIN2855525) IP Image Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treat...
Immunofluorescence (IF) image for anti-p53 antibody (Tumor Protein P53) (Center) (ABIN2855525) ICC/IF Image p53 antibody detects p53 protein at nucleus by immunofluorescent analysi...
Chromatin Immunoprecipitation (ChIP) image for anti-p53 antibody (Tumor Protein P53) (Center) (ABIN2855525) ChIP Image p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiment...