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|Application / Reactivity||Human|
|Affinity Purification (AP)||2 Antibodies|
|Cellular Assay (CA)||1 Antibodies|
|Chromatin Immunoprecipitation (ChIP)||10 Antibodies|
|Dot Blot (DB)||31 Antibodies|
|ELISA (Capture)||2 Antibodies|
|ELISA (Detection)||2 Antibodies|
|Enzyme Immunoassay (EIA)||17 Antibodies|
|Flow Cytometry (FACS)||220 Antibodies|
|Gel Shift (GS)||3 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunochromatography (IC)||35 Antibodies|
|Immunocytochemistry (ICC)||78 Antibodies|
|Immunodiffusion (ID)||16 Antibodies|
|Immunofluorescence (IF)||309 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||160 Antibodies|
|Immunohistochemistry (IHC)||511 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||11 Antibodies|
|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Dot Blot (DB), Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling References for anti-p53 Antibody (ABIN1881624) Images
|Purification||This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)|
|Immunogen||This P53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S9 of human P53.|
Target Details p53Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN1881624) Images back to top
|Alternative Name||P53 (TP53 Antibody Abstract)|
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.
Synonyms: TP53, P53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
|NCBI Accession||NP_001263628, NP_001263627, NP_001119588, NP_001119589, NP_00126, NP_001119585, NP_001119584, NP_001119587, NP_001119586, NP_001263624, NP_001119590, NP_000537, NP_001263625, NP_001263626|
|Research Area||Transcription Factors|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling|
Application DetailsProduct Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN1881624) Images back to top
|Application Notes||DB: 1:500,WB: 1:1000,IF: 1:10-50|
|Restrictions||For Research Use only|
HandlingProduct Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN1881624) Images back to top
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
References for anti-p53 Antibody (ABIN1881624)Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
|Product cited in:||
Blanchette, Cheng, Yan et al.: "Both BC-box motifs of adenovirus protein E4orf6 are required to efficiently assemble an E3 ligase complex that degrades p53." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9619-29, 2004 (PubMed).
Montagnoli, Tenca, Sola et al.: "Cdc7 inhibition reveals a p53-dependent replication checkpoint that is defective in cancer cells." in: Cancer research, Vol. 64, Issue 19, pp. 7110-6, 2004 (PubMed).
Anazawa, Arakawa, Nakagawa et al.: "Identification of STAG1 as a key mediator of a p53-dependent apoptotic pathway." in: Oncogene, Vol. 23, Issue 46, pp. 7621-7, 2004 (PubMed).
Adachi, Toyota, Sasaki et al.: "Identification of SCN3B as a novel p53-inducible proapoptotic gene." in: Oncogene, Vol. 23, Issue 47, pp. 7791-8, 2004 (PubMed).
Zhang, Wang, Chen et al.: "Repression of hsp90beta gene by p53 in UV irradiation-induced apoptosis of Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 41, pp. 42545-51, 2004 (PubMed).
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