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anti-Human p53 antibody for Dot Blot

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
pSer9
164
110
89
64
64
63
54
51
51
50
48
46
40
39
31
30
27
26
25
22
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
9
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2048
381
310
124
102
93
56
54
27
23
19
16
13
11
10
10
5
4
4
3
3
2
2
1
Host
Rabbit
1240
916
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Dot Blot (DB), Immunofluorescence (IF), Western Blotting (WB)
1657
717
558
488
436
428
397
237
196
156
53
38
35
17
16
13
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN1881624
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.63784635 ABIN389620 DB IHC (p) Rabbit Ig pSer315 Log in to see Polyclonal
0.63784635 ABIN389626 DB IHC (p) WB Rabbit Ig pThr18 Log in to see Polyclonal
0.63784635 ABIN389876 DB Rabbit Ig pThr55 Log in to see Polyclonal
0.63784635 ABIN2491359 DB IHC Rabbit Ig Fraction pSer315 Log in to see Polyclonal
0.63784635 ABIN2491367 DB IHC WB Rabbit Ig Fraction pThr18 Log in to see Polyclonal
0.63784635 ABIN2497397 DB ELISA Rabbit Ig Fraction Log in to see Polyclonal
0.0008934913 ABIN1953124 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG Thr55 Log in to see Polyclonal
0.0008934913 ABIN1953996 DB ELISA APC Rabbit IgG Thr55 Log in to see Polyclonal
0.0008934913 ABIN1958769 DB ELISA PE Rabbit IgG Thr55 Log in to see Polyclonal
0.0008934913 ABIN2575370 DB Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN2328352 DB ELISA APC Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN2328361 DB ELISA HRP Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN2328358 DB ELISA FITC Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN2328364 DB ELISA PE Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN2328206 DB ELISA Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN1840228 DB IHC (p) WB Rabbit pThr18 Log in to see Polyclonal
0.0008934913 ABIN2328348 DB ELISA Alkaline Phosphatase (AP) Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN2328095 DB WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008934913 ABIN2328355 DB ELISA Biotin Rabbit IgG pThr55 Log in to see Polyclonal
0.0008934913 ABIN1840186 DB Rabbit pThr55 Log in to see Polyclonal

Top referenced anti-p53 antibody for Dot Blot

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Application / Reactivity Human
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA 665 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 382 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies
Immunocytochemistry (ICC) 191 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 429 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 541 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope pSer9
(164), (110), (89), (64), (64), (63), (54), (51), (51), (50), (48), (46), (40), (39), (31), (30), (27), (26), (25), (22), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2048), (381), (310), (124), (102), (93), (56), (54), (27), (23), (19), (16), (13), (11), (10), (10), (5), (4), (4), (3), (3), (2), (2), (1)
Host Rabbit
(1240), (916), (7), (4), (3), (1), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Dot Blot (DB), Immunofluorescence (IF), Western Blotting (WB)
(1657), (717), (558), (488), (436), (428), (397), (237), (196), (156), (53), (38), (35), (17), (16), (13), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN1881624) Images
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Immunogen This P53 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S9 of human P53.
Clone RB7893

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN1881624) Images back to top
Antigen
Alternative Name P53 (TP53 Antibody Abstract)
Background Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.

Synonyms: TP53, P53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
NCBI Accession NP_001263628, NP_001263627, NP_001119588, NP_001119589, NP_00126, NP_001119585, NP_001119584, NP_001119587, NP_001119586, NP_001263624, NP_001119590, NP_000537, NP_001263625, NP_001263626
UniProt P04637
Research Area Transcription Factors
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN1881624) Images back to top
Application Notes DB: 1:500,WB: 1:1000,IF: 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN1881624) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

References for anti-p53 Antibody (ABIN1881624)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Blanchette, Cheng, Yan, Ketner, Ornelles, Dobner, Conaway, Conaway, Branton: "Both BC-box motifs of adenovirus protein E4orf6 are required to efficiently assemble an E3 ligase complex that degrades p53." in: Molecular and cellular biology, Vol. 24, Issue 21, pp. 9619-29, 2004

Adachi, Toyota, Sasaki, Yamashita, Ishida, Ohe-Toyota, Maruyama, Hinoda, Saito, Imai, Kudo, Tokino: "Identification of SCN3B as a novel p53-inducible proapoptotic gene." in: Oncogene, Vol. 23, Issue 47, pp. 7791-8, 2004

Zhang, Wang, Chen, Zhang, Cheng, Heng, Wu, Shen: "Repression of hsp90beta gene by p53 in UV irradiation-induced apoptosis of Jurkat cells." in: The Journal of biological chemistry, Vol. 279, Issue 41, pp. 42545-51, 2004

Anazawa, Arakawa, Nakagawa, Nakamura: "Identification of STAG1 as a key mediator of a p53-dependent apoptotic pathway." in: Oncogene, Vol. 23, Issue 46, pp. 7621-7, 2004

Montagnoli, Tenca, Sola, Carpani, Brotherton, Albanese, Santocanale: "Cdc7 inhibition reveals a p53-dependent replication checkpoint that is defective in cancer cells." in: Cancer research, Vol. 64, Issue 19, pp. 7110-6, 2004

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN1881624) back to top
Supplier Images
Dot Blot (DB) image for anti-p53 antibody (Tumor Protein P53) (pSer9) (ABIN1881624) Western blot analysis of Phospho-P53-S9 (ABIN1881624) in HL60(left) and Ramos (right)...
Immunofluorescence (IF) image for anti-p53 antibody (Tumor Protein P53) (pSer9) (ABIN1881624) Western blot analysis of Phospho-P53-S9 (ABIN1881624) in HL60(left) and Ramos (right)...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (pSer9) (ABIN1881624) Western blot analysis of Phospho-P53-S9 (ABIN1881624) in HL60(left) and Ramos (right)...