You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human p53 antibody for Enzyme Immunoassay

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
Ser315
156
105
87
67
67
65
56
54
52
51
46
46
42
40
31
31
28
25
24
23
22
21
19
17
17
13
13
13
13
13
12
11
11
10
10
9
9
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2129
409
327
121
101
89
57
55
29
23
19
16
15
10
10
5
5
4
4
4
3
3
3
2
2
Host
Rabbit
1287
955
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
70
64
51
46
33
32
23
23
18
18
18
17
16
15
15
15
15
15
14
14
14
14
14
14
14
14
7
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1694
744
620
536
501
448
386
256
250
156
54
39
35
18
16
16
13
12
11
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN358189
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358423 EIA Rabbit Ig pThr55 Log in to see Polyclonal 5
1 ABIN358762 EIA IHC (p) Rabbit Ig Ser315 Log in to see Polyclonal 5
1 ABIN120054 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 3
1 ABIN358195 EIA IHC (p) WB Rabbit Ig pThr18 Log in to see Polyclonal 5
1 ABIN358188 EIA IHC (p) WB Rabbit Ig pSer20 Log in to see Polyclonal 5
1 ABIN358757 EIA WB Rabbit Ig C-Term Log in to see Polyclonal 5
1 ABIN111971 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG2a AA 16-25 Log in to see BP53-12 8
1 ABIN320175 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
1 ABIN264401 EIA IP WB Mouse IgG1 AA 16-25 Log in to see X77 2
1 ABIN4620408 EIA WB Rabbit Ig Ser15 Log in to see Polyclonal 5
1 ABIN4620409 EIA WB Rabbit Ig Thr55 Log in to see Polyclonal 5
1 ABIN264449 EIA IHC (fro) IP WB Mouse IgG2b AA 371-380 Log in to see HR231
1 ABIN452450 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG2a AA 20-31 Log in to see Bp53-11 8
1 ABIN126847 EIA WB Mouse IgG1 phosphorylated Log in to see 9F4
1 ABIN492445 EIA FACS IHC (fro) IHC (p) IP RIA WB Mouse IgG1 AA 46-53 Log in to see Pab 1801
1 ABIN271769 EIA WB Rabbit Log in to see Polyclonal

Top referenced anti-p53 antibody for Enzyme Immunoassay

Similar anti-p53 Antibodies

Application / Reactivity Human
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 32 Antibodies
ELISA 689 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 428 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies
Immunocytochemistry (ICC) 230 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 477 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 568 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Ser315
(156), (105), (87), (67), (67), (65), (56), (54), (52), (51), (46), (46), (42), (40), (31), (31), (28), (25), (24), (23), (22), (21), (19), (17), (17), (13), (13), (13), (13), (13), (12), (11), (11), (10), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2129), (409), (327), (121), (101), (89), (57), (55), (29), (23), (19), (16), (15), (10), (10), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2)
Host Rabbit
(1287), (955), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(70), (64), (51), (46), (33), (32), (23), (23), (18), (18), (18), (17), (16), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1694), (744), (620), (536), (501), (448), (386), (256), (250), (156), (54), (39), (35), (18), (16), (16), (13), (12), (11), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN358189) Images
Specificity This antibody detects p53 pSer315.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is Peptide Affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser315 of Human p53.
Isotype Ig

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN358189) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157, 9606
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN358189) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN358189) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-p53 Antibody (ABIN358189)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Seemann, Hainaut: "Roles of thioredoxin reductase 1 and APE/Ref-1 in the control of basal p53 stability and activity." in: Oncogene, Vol. 24, Issue 24, pp. 3853-63, 2005

Mann, Hainaut: "Aminothiol WR1065 induces differential gene expression in the presence of wild-type p53." in: Oncogene, Vol. 24, Issue 24, pp. 3964-75, 2005

Garden, Morrison: "The multiple roles of p53 in the pathogenesis of HIV associated dementia." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 799-809, 2005

Castedo, Perfettini, Piacentini, Kroemer: "p53-A pro-apoptotic signal transducer involved in AIDS." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 701-6, 2005

Duensing, Duensing: "Guilt by association? p53 and the development of aneuploidy in cancer." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 694-700, 2005

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN358189) back to top
Supplier Images
 image for anti-p53 antibody (Tumor Protein P53) (Ser315) (ABIN358189) anti-Tumor Protein P53 (TP53) (Ser315) antibody
 image for anti-p53 antibody (Tumor Protein P53) (Ser315) (ABIN358189) anti-Tumor Protein P53 (TP53) (Ser315) antibody (Image 2)