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anti-Human p53 antibody for ELISA

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
N-Term
164
109
89
64
64
63
54
51
51
50
48
46
41
39
31
30
27
26
25
22
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
9
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2048
381
310
124
102
93
56
54
27
23
19
16
13
11
10
10
5
4
4
3
3
2
2
1
Host
Rabbit
1240
916
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)
1657
716
558
488
437
428
397
237
196
156
53
39
35
17
16
12
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN2779330
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32748017 ABIN521046 IHC (p) PLA RNAi ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C11
0.32748017 ABIN563227 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C3
0.32748017 ABIN1847562 IHC ELISA Rabbit C-Term, pThr387 Log in to see Polyclonal
0.0012092679 ABIN2779319 ChIP ELISA WB Rabbit N-Term Log in to see Polyclonal 1
0.0012092679 ABIN1531937 ELISA WB Rabbit IgG pSer37 Log in to see Polyclonal 1
0.0012092679 ABIN1531939 IHC ELISA WB Rabbit IgG pSer6 Log in to see Polyclonal 1
0.0012092679 ABIN863095 FACS IP IHC RIA ELISA WB Mouse AA 46-53 Log in to see Pab 1801 4
0.0012092679 ABIN1532971 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012092679 ABIN969336 IHC ELISA WB Mouse IgG1 Log in to see 4A8 2
0.0012092679 ABIN3201005 IHC (p) ELISA WB Mouse IgG1 kappa pSer46 Log in to see #36 3
0.0012092679 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see PAb 1801 5
0.0012092679 ABIN94448 ICC IHC (p) IP ELISA WB Mouse IgG2a AA 16-25 Log in to see BP53-12 7
0.0012092679 ABIN2482452 FACS IP RIA IHC ELISA WB FITC Mouse AA 46-53 Log in to see Pab 1801 1
0.0012092679 ABIN2482455 FACS IP RIA IHC ELISA WB PerCP Mouse AA 46-53 Log in to see Pab 1801 1
0.0012092679 ABIN2482442 FACS IP RIA IHC ELISA WB Atto 488 Mouse AA 46-53 Log in to see Pab 1801 1
0.0012092679 ABIN2482456 FACS IP RIA IHC ELISA WB PE Mouse AA 46-53 Log in to see Pab 1801 1
0.0012092679 ABIN2482457 FACS IP RIA IHC ELISA WB Streptavidin Mouse AA 46-53 Log in to see Pab 1801 1
0.0012092679 ABIN2482453 FACS IP RIA IHC ELISA WB HRP Mouse AA 46-53 Log in to see Pab 1801 1
0.0012092679 ABIN2482449 FACS IP RIA IHC ELISA WB Alkaline Phosphatase (AP) Mouse AA 46-53 Log in to see Pab 1801 1
0.0012092679 ABIN2482447 FACS IP RIA IHC ELISA WB Atto 680 Mouse AA 46-53 Log in to see Pab 1801 1

Top referenced anti-p53 antibody for ELISA

Similar anti-p53 Antibodies

Application / Reactivity Human
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA 665 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 382 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies
Immunocytochemistry (ICC) 191 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 429 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 541 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope N-Term
(164), (109), (89), (64), (64), (63), (54), (51), (51), (50), (48), (46), (41), (39), (31), (30), (27), (26), (25), (22), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2048), (381), (310), (124), (102), (93), (56), (54), (27), (23), (19), (16), (13), (11), (10), (10), (5), (4), (4), (3), (3), (2), (2), (1)
Host Rabbit
(1240), (916), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)
(1657), (716), (558), (488), (437), (428), (397), (237), (196), (156), (53), (39), (35), (17), (16), (12), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2779330) Images
Blocking Peptide Blocking peptide for this product available: ABIN985385
Sequence EEPQSDPSVE PPLSQETFSD LWKLLPENNV LSPLPSQAMD DLMLSPDDIE
Predicted Reactivity Human
Characteristics This is a rabbit polyclonal antibody against TP53. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TP53

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN2779330) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background TP53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
Molecular Weight 44 kDa
Gene ID 7157
NCBI Accession NM_000546, NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN2779330) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 393 AA

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN2779330) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-p53 Antibody (ABIN2779330)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Boehme, Kulikov, Blattner: "p53 stabilization in response to DNA damage requires Akt/PKB and DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7785-90, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2779330) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) WB Suggested Anti-TP53 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) WB Suggested Anti-TP53 Antibody
Titration: 1 ug/ml
Positive C...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) anti-Tumor Protein P53 (TP53) (N-Term) antibody (Image 3)
ELISA image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) anti-Tumor Protein P53 (TP53) (N-Term) antibody (Image 4)