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anti-Human p53 antibody for ELISA

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
N-Term
153
98
88
69
66
66
57
56
55
53
49
47
43
42
32
30
30
28
25
24
23
21
21
20
17
17
15
15
15
15
15
12
11
11
10
10
9
8
8
8
8
7
7
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2212
496
410
170
130
94
69
64
35
23
18
17
17
16
16
11
5
4
4
4
4
3
3
3
2
1
Host
Rabbit
1309
995
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
75
70
57
48
36
35
24
24
21
21
19
18
18
18
18
17
17
17
17
16
16
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
3
3
2
2
1
Application
Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)
1782
750
660
587
535
473
412
267
259
179
55
41
34
31
18
17
15
14
13
12
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
1
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Catalog No. ABIN2779330
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.229799 ABIN521046 IHC (p) PLA RNAi ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C11
11.229799 ABIN563227 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C3
11.229799 ABIN1847562 IHC ELISA Rabbit C-Term, pThr387 Log in to see Polyclonal
1 ABIN2779319 ChIP ELISA WB Rabbit N-Term Log in to see Polyclonal 1
1 ABIN1531937 ELISA WB Rabbit IgG pSer37 Log in to see Polyclonal 1
1 ABIN1531939 IHC ELISA WB Rabbit IgG pSer6 Log in to see Polyclonal 1
1 ABIN863095 FACS IP IHC RIA ELISA WB Mouse IgG1 AA 46-53 Log in to see Pab 1801 4
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 19
1 ABIN1532971 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see Pab1801 7
1 ABIN969336 IHC ELISA WB Mouse IgG1 Log in to see 4A8 2
1 ABIN4219254 ELISA IHC (fp) IHC (fro) IP WB Mouse IgG2a AA 20-31 Log in to see Bp53-11 9
1 ABIN3201005 IHC (p) ELISA WB Mouse IgG1 kappa pSer46 Log in to see #36 3
1 ABIN2482451 FACS IP RIA IHC ELISA WB Biotin Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482441 FACS IP RIA IHC ELISA WB Atto 390 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482443 FACS IP RIA IHC ELISA WB Atto 565 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482447 FACS IP RIA IHC ELISA WB Atto 680 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482444 FACS IP RIA IHC ELISA WB Atto 594 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482446 FACS IP RIA IHC ELISA WB Atto 655 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1
1 ABIN2482448 FACS IP RIA IHC ELISA WB Atto 700 Mouse IgG1 AA 46-53 Log in to see Pab 1801 1

Top referenced anti-p53 antibody for ELISA

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Application / Reactivity Human
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Cytometry by Time of Flight (CyTOF) 18 Antibodies
Dot Blot (DB) 30 Antibodies
ELISA 698 Antibodies
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ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 465 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
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Immunocytochemistry (ICC) 249 Antibodies
Immunodiffusion (ID) 15 Antibodies
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Immunofluorescence (fixed cells) (IF/ICC) 28 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 179 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope N-Term
(153), (98), (88), (69), (66), (66), (57), (56), (55), (53), (49), (47), (43), (42), (32), (30), (30), (28), (25), (24), (23), (21), (21), (20), (17), (17), (15), (15), (15), (15), (15), (12), (11), (11), (10), (10), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2212), (496), (410), (170), (130), (94), (69), (64), (35), (23), (18), (17), (17), (16), (16), (11), (5), (4), (4), (4), (4), (3), (3), (3), (2), (1)
Host Rabbit
(1309), (995), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(75), (70), (57), (48), (36), (35), (24), (24), (21), (21), (19), (18), (18), (18), (18), (17), (17), (17), (17), (16), (16), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Application Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)
(1782), (750), (660), (587), (535), (473), (412), (267), (259), (179), (55), (41), (34), (31), (18), (17), (15), (14), (13), (12), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2779330) Images
Blocking Peptide Blocking peptide for this product available: ABIN985385
Sequence EEPQSDPSVE PPLSQETFSD LWKLLPENNV LSPLPSQAMD DLMLSPDDIE
Predicted Reactivity Human
Characteristics This is a rabbit polyclonal antibody against TP53. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TP53

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN2779330) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background TP53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
Molecular Weight 44 kDa
Gene ID 7157
NCBI Accession NM_000546, NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN2779330) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 393 AA

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN2779330) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-p53 Antibody (ABIN2779330)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Boehme, Kulikov, Blattner: "p53 stabilization in response to DNA damage requires Akt/PKB and DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7785-90, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2779330) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) WB Suggested Anti-TP53 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) WB Suggested Anti-TP53 Antibody
Titration: 1 ug/ml
Positive C...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) anti-Tumor Protein P53 (TP53) (N-Term) antibody (Image 3)
ELISA image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779330) anti-Tumor Protein P53 (TP53) (N-Term) antibody (Image 4)