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anti-Human p53 antibody for Immunocytochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
Ser20
156
105
87
67
67
65
56
54
52
51
46
46
42
40
31
31
28
25
25
23
22
21
19
17
17
13
13
13
13
13
12
11
10
10
10
9
9
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2129
408
326
121
101
89
57
55
29
23
19
16
15
10
10
5
5
4
4
4
3
3
3
2
2
Host
Mouse
1288
954
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
70
64
51
46
33
32
23
23
18
18
18
17
16
15
15
15
15
15
14
14
14
14
14
14
14
14
7
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1694
744
619
537
501
448
385
256
249
156
54
39
35
18
17
16
13
12
11
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN222890
$ 336.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 19
1 ABIN152309 ChIP FACS ICC IF IHC IHC (p) IP PLA WB Rabbit IgG AA 50-100 Log in to see Polyclonal 7
1 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
1 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal 2
1 ABIN94449 ELISA ICC IHC (p) IP WB FITC Mouse IgG2a AA 16-25 Log in to see BP53-12 7
1 ABIN94448 ICC IHC (p) IP ELISA WB Mouse IgG2a AA 16-25 Log in to see BP53-12 7
1 ABIN2854816 ICC IF IP IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1502043 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see IMD-53
1 ABIN3043664 ICC IHC (fro) IHC (p) WB Mouse IgG2a Log in to see BP53-12
1 ABIN2855525 ChIP ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN205508 ICC IHC (p) IP ELISA WB Mouse IgG2a Log in to see BP53-12
1 ABIN1723457 ICC IF IHC (p) IP ELISA WB Mouse IgG2a kappa Log in to see BP53-12
1 ABIN1740217 ELISA ICC IF IHC (p) Rabbit IgG acLys379 Log in to see Polyclonal
1 ABIN1740220 ICC IF IHC (p) ELISA WB Rabbit IgG AA 344-393 Log in to see Polyclonal
1 ABIN4900613 FACS ICC Mouse IgG1 pSer15 Log in to see 261366
1 ABIN4342826 ICC IF IHC IHC (p) SimWes WB Mouse IgG1 Log in to see CL2199
1 ABIN4342749 ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN206927 ICC IHC (p) IP ELISA WB Mouse IgG2a AA 16-25 Log in to see BP53-12
1 ABIN1822768 ICC FACS IF IHC (p) IP Mouse IgG2a Log in to see NOD-15
1 ABIN4342790 FACS ICC IF IHC (p) WB Mouse IgG2a AA 20-25 Log in to see SPM589

Top referenced anti-p53 antibody for Immunocytochemistry

Similar anti-p53 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 4 Antibodies 4 Antibodies
ELISA 115 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 43 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 47 Antibodies
Immunofluorescence (IF) 86 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 46 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 231 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 10 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Ser20
(156), (105), (87), (67), (67), (65), (56), (54), (52), (51), (46), (46), (42), (40), (31), (31), (28), (25), (25), (23), (22), (21), (19), (17), (17), (13), (13), (13), (13), (13), (12), (11), (10), (10), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2129), (408), (326), (121), (101), (89), (57), (55), (29), (23), (19), (16), (15), (10), (10), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2)
Host Mouse
(1288), (954), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(70), (64), (51), (46), (33), (32), (23), (23), (18), (18), (18), (17), (16), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1694), (744), (619), (537), (501), (448), (385), (256), (249), (156), (54), (39), (35), (18), (17), (16), (13), (12), (11), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN222890) Images
Blocking Peptide Blocking peptide for this product available: ABIN412862
Purification Affinity purified
Immunogen Synthetic peptide corresponding to residue surrounding Ser20 of human p53
Clone C6A5
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN222890) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Synonyms: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN222890) Images back to top
Application Notes Western blotting (0.5-4 μg/mL), Immunoprecipitation (4-8 μg/mL), Immunohistochemistry (4-8 μg/mL), Immunocytochemistry (4-8 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: USA

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN222890) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) in PBS containing 1 mg/mL BSA and 1.5 mM sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-p53 Antibody (ABIN222890)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Javed, Khan, Khan, Vaibhav, Ahmad, Khuwaja, Ahmed, Raza, Ashafaq, Tabassum, Siddiqui, El-Agnaf, Safhi, Islam: "S-allyl cysteine attenuates oxidative stress associated cognitive impairment and neurodegeneration in mouse model of streptozotocin-induced experimental dementia of Alzheimer's type." in: Brain research, Vol. 1389, pp. 133-42, 2011

Khan, Ishrat, Ahmad, Hoda, Khan, Khuwaja, Srivastava, Raza, Islam, Ahmad: "Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 255-63, 2009

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN222890) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (Ser20) (ABIN222890) Western blot analysis of p53 in Cos cell lysate.