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anti-Human p53 antibody for Intracellular Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Reactivity
Human
2058
383
317
120
101
89
56
54
29
23
19
15
13
10
10
5
5
4
4
3
3
3
3
2
2
Host
Mouse
1230
925
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
68
61
49
45
31
31
23
23
17
17
16
16
16
14
14
14
14
14
14
13
13
13
13
13
12
12
6
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Intracellular Flow Cytometry (ICFC)
1649
732
558
492
432
418
396
230
192
156
53
38
35
17
16
12
11
11
11
9
9
3
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2664005
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008670244 ABIN2664003 ICFC Mouse IgG2a kappa Log in to see BP53-12 6
0.0008670244 ABIN2664004 ICFC Mouse IgG2a kappa Log in to see BP53-12 6
0.0008670244 ABIN2664006 ICFC Mouse IgG2b Log in to see DO-7 2

Top referenced anti-p53 antibody for Intracellular Flow Cytometry

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General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Human
(2058), (383), (317), (120), (101), (89), (56), (54), (29), (23), (19), (15), (13), (10), (10), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Mouse
(1230), (925), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(68), (61), (49), (45), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (12), (12), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(1649), (732), (558), (492), (432), (418), (396), (230), (192), (156), (53), (38), (35), (17), (16), (12), (11), (11), (11), (9), (9), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2664005) Images
Purification This antibody was purified by affinity chromatography.
Clone DO-7
Isotype IgG2b

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN2664005) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation, ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN2664005) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN2664005) Images back to top
Concentration 0.5 mg/mL
Buffer phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-p53 Antibody (ABIN2664005)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Stephen, Helminen, Lane: "Characterisation of epitopes on human p53 using phage-displayed peptide libraries: insights into antibody-peptide interactions." in: Journal of molecular biology, Vol. 248, Issue 1, pp. 58-78, 1995

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2664005) back to top
Supplier Images
Immunofluorescence (IF) image for anti-p53 antibody (Tumor Protein P53) (ABIN2664005) anti-Tumor Protein P53 (TP53) antibody
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (ABIN2664005) anti-Tumor Protein P53 (TP53) antibody (Image 2)