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anti-Human p53 antibody for Intracellular Flow Cytometry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Reactivity
Human, Non Human Primates, Non-Human Primate
2048
381
310
124
102
93
56
54
27
23
19
16
13
11
10
10
5
4
3
3
3
2
1
1
Host
Mouse
1241
915
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Intracellular Flow Cytometry (ICFC)
1658
717
558
488
437
428
397
237
196
156
53
39
35
17
16
13
12
11
11
9
9
3
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN2664003
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087393454 ABIN2664005 ICFC Mouse IgG2b Log in to see DO-7 2
0.00087393454 ABIN2664004 ICFC Mouse IgG2a kappa Log in to see BP53-12 6
0.00087393454 ABIN2664006 ICFC Mouse IgG2b Log in to see DO-7 2

Top referenced anti-p53 antibody for Intracellular Flow Cytometry

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Application / Reactivity Human Non-Human Primate Non Human Primates
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Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 54 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies 2 Antibodies 2 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 402 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 486 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 541 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 233 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Human, Non Human Primates, Non-Human Primate
(2048), (381), (310), (124), (102), (93), (56), (54), (27), (23), (19), (16), (13), (11), (10), (10), (5), (4), (3), (3), (3), (2), (1), (1)
Host Mouse
(1241), (915), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(1658), (717), (558), (488), (437), (428), (397), (237), (196), (156), (53), (39), (35), (17), (16), (13), (12), (11), (11), (9), (9), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2664003) Images
Purification This antibody was purified by affinity chromatography.
Clone BP53-12
Isotype IgG2a kappa

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN2664003) Images back to top
Antigen
Alternative Name P53 (TP53 Antibody Abstract)
Background P53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation, ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.
Research Area Transcription Factors
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN2664003) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN2664003) Images back to top
Concentration 0.5 mg/mL
Buffer Tris-buffered solution, pH 8.0, containing 0.09 % sodium azide at 0.5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-p53 Antibody (ABIN2664003)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Lanoix, Lacasse, Reiter, Vaillancourt: "Melatonin: the watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis." in: Molecular and cellular endocrinology, Vol. 381, Issue 1-2, pp. 35-45, 2013

Mihara, Erster, Zaika, Petrenko, Chittenden, Pancoska, Moll: "p53 has a direct apoptogenic role at the mitochondria." in: Molecular cell, Vol. 11, Issue 3, pp. 577-90, 2003

Saito, Yamaguchi, Higashimoto, Chao, Xu, Fornace, Appella, Anderson: "Phosphorylation site interdependence of human p53 post-translational modifications in response to stress." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37536-44, 2003

Shieh, Ikeda, Taya, Prives: "DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2." in: Cell, Vol. 91, Issue 3, pp. 325-34, 1997

Terada, Shimizu, Izumi, Nakanuma et al.: "Methods in pathology. p53 expression in formalin-fixed, paraffin-embedded archival specimens of intrahepatic cholangiocarcinoma: retrieval of p53 antigenicity by microwave oven heating of tissue ..." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 7, Issue 2, pp. 249-52, 1994

Vogelstein, Kinzler: "p53 function and dysfunction." in: Cell, Vol. 70, Issue 4, pp. 523-6, 1992

Images

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Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (ABIN2664003) anti-Tumor Protein P53 (TP53) antibody