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|Application / Reactivity||Human||Non-Human Primate||Non Human Primates|
|Western Blotting (WB)||1307 Antibodies||1 Antibodies|
|Transfected Cell Culture (TCC)||1 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Radioimmunoassay (RIA)||54 Antibodies|
|Proximity Ligation Assay (PLA)||4 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Intracellular Flow Cytometry (ICFC)||4 Antibodies||2 Antibodies||2 Antibodies|
|Immunostaining (ISt)||1 Antibodies|
|Immunoprecipitation (IP)||288 Antibodies||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||301 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||510 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||94 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||9 Antibodies|
|Antigen||Tumor Protein P53 (TP53) Antibodies|
|Reactivity||Human, Non Human Primates, Non-Human Primate Alternatives|
|Conjugate||This p53 antibody is un-conjugated Alternatives|
Intracellular Flow Cytometry (ICFC)
|6 references available|
|Supplier||Log in to see|
Product Details anti-p53 AntibodyTarget Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2664003) Images
|Purification||This antibody was purified by affinity chromatography.|
Target Details p53Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN2664003) Images back to top
|Alternative Name||P53 (TP53 Antibody Abstract)|
|Background||P53 is a 53 kD protein that forms tetramers and functions as a tumor suppressor and transcriptional activator of genes that inhibit growth and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion. This protein is localized to the nucleus when activated and can be upregulated by genotoxic or other cellular stresses. p53 is modified by phosphorylation, acetylation, ribosylation, ubiquitination, and sumoylation, ubiquination targets p53 for degradation via mdm2. This protein interacts with a variety of proteins including mdm2, mdmx, topoisomerase I, PML3, Bcl-XL, Bcl-2, Chk1, JNK, p38, HIPK2, CK2, DNA-PK, p300/CBP, PCAF, PARP1, and HDAC1-3. Mutant p53 associates with p63 and p73.|
|Research Area||Transcription Factors|
|Pathways||p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling|
Application DetailsProduct Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN2664003) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN2664003) Images back to top
|Buffer||Tris-buffered solution, pH 8.0, containing 0.09 % sodium azide at 0.5 mg/mL.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||The antibody solution should be stored undiluted between 2°C and 8°C.|
References for anti-p53 Antibody (ABIN2664003)Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Lanoix, Lacasse, Reiter et al.: "Melatonin: the watchdog of villous trophoblast homeostasis against hypoxia/reoxygenation-induced oxidative stress and apoptosis." in: Molecular and cellular endocrinology, Vol. 381, Issue 1-2, pp. 35-45, 2013 (PubMed).
Saito, Yamaguchi, Higashimoto et al.: "Phosphorylation site interdependence of human p53 post-translational modifications in response to stress." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37536-44, 2003 (PubMed).
Mihara, Erster, Zaika et al.: "p53 has a direct apoptogenic role at the mitochondria." in: Molecular cell, Vol. 11, Issue 3, pp. 577-90, 2003 (PubMed).
Shieh, Ikeda, Taya et al.: "DNA damage-induced phosphorylation of p53 alleviates inhibition by MDM2." in: Cell, Vol. 91, Issue 3, pp. 325-34, 1997 (PubMed).
Terada, Shimizu, Izumi et al.: "Methods in pathology. p53 expression in formalin-fixed, paraffin-embedded archival specimens of intrahepatic cholangiocarcinoma: retrieval of p53 antigenicity by microwave oven heating of tissue ..." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 7, Issue 2, pp. 249-52, 1994 (PubMed).
Vogelstein, Kinzler: "p53 function and dysfunction." in: Cell, Vol. 70, Issue 4, pp. 523-6, 1992 (PubMed).
ImagesProduct Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2664003) back to top