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anti-Human p53 antibody for Immunofluorescence (Cultured Cells)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 240-290, Wild Type
156
106
87
69
67
67
58
56
53
53
48
46
44
42
31
31
30
25
25
24
23
21
21
20
17
16
15
15
15
15
14
12
11
11
10
10
9
8
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2173
440
334
121
101
89
57
55
29
19
18
16
15
10
10
7
5
4
4
4
3
3
3
2
2
Host
Rabbit
1323
960
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
71
66
53
47
34
33
23
23
19
19
19
17
16
16
16
16
16
16
15
15
15
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
4
3
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1710
739
638
544
532
464
391
281
253
178
55
39
35
18
17
16
13
12
12
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
Options
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Catalog No. ABIN723935
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2808148 IF (cc) IF (p) Alexa Fluor 594 Rabbit IgG AA 240-290 Log in to see Polyclonal
1 ABIN723938 IF (cc) IF (p) Cy3 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN723939 IF (cc) IF (p) Cy5 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN723942 IF (cc) IF (p) FITC Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN723940 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN723941 IF (cc) IF (p) Cy7 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN909770 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN909772 IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN909773 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN909774 IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 240-290, Wild Type Log in to see Polyclonal
1 ABIN5008199 IF (cc) IF (p) Alexa Fluor 680 Rabbit IgG AA 240-290 Log in to see Polyclonal
1 ABIN5008200 IF (cc) IF (p) Alexa Fluor 750 Rabbit IgG AA 240-290 Log in to see Polyclonal

Top referenced anti-p53 antibody for Immunofluorescence (Cultured Cells)

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Application / Reactivity Rat (Rattus) Mouse (Murine)
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Flow Cytometry (FACS) 43 Antibodies
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Immunocytochemistry (ICC) 47 Antibodies
Immunofluorescence (IF) 86 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 73 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 52 Antibodies
Immunoprecipitation (IP) 47 Antibodies
Intracellular Staining (ICS) 1 Antibodies
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General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 240-290, Wild Type
(156), (106), (87), (69), (67), (67), (58), (56), (53), (53), (48), (46), (44), (42), (31), (31), (30), (25), (25), (24), (23), (21), (21), (20), (17), (16), (15), (15), (15), (15), (14), (12), (11), (11), (10), (10), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2173), (440), (334), (121), (101), (89), (57), (55), (29), (19), (18), (16), (15), (10), (10), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2)
Host Rabbit
(1323), (960), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(71), (66), (53), (47), (34), (33), (23), (23), (19), (19), (19), (17), (16), (16), (16), (16), (16), (16), (15), (15), (15), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1710), (739), (638), (544), (532), (464), (391), (281), (253), (178), (55), (39), (35), (18), (17), (16), (13), (12), (12), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN723935) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human P53
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN723935) Images back to top
Antigen
Alternative Name P53 Protein (TP53 Antibody Abstract)
Background

Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.

Subcellular location: Cytoplasm, Nucleus


Synonyms: P53, BCC7, LFS1, TRP53, Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53
Gene ID 7157
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN723935) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN723935) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-p53 Antibody (ABIN723935)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Wang, Liu, Liu, Chen, Liu, Kuang: "Hypoxia Inhibits Myogenic Differentiation through p53 Protein-dependent Induction of Bhlhe40 Protein." in: The Journal of biological chemistry, Vol. 290, Issue 50, pp. 29707-16, 2015

Hong, Shen, Liu, Li, Huang, Shi: "Developmental toxicity of three hexabromocyclododecane diastereoisomers in embryos of the marine medaka Oryzias melastigma." in: Marine pollution bulletin, Vol. 101, Issue 1, pp. 110-8, 2015

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, 2012 (1:1000).

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN723935) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-p53 antibody (Tumor Protein P53) (AA 240-290) (ABIN723935) Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti...
SDS-PAGE (SDS) image for anti-p53 antibody (Tumor Protein P53) (AA 240-290) (ABIN723935) 1 rat brain, L2 human colon carcinoma lysates probed (ABIN723935) at 1:200 in 4 °C. F...
Immunofluorescence (IF) image for anti-p53 antibody (Tumor Protein P53) (AA 240-290) (ABIN723935) Image kindly submitted by Piotr Mamczur from Wroclaw University. Immunofluorescent lo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (AA 240-290) (ABIN723935) Formalin-fixed and paraffin embedded human ovarian carcinoma labeled with Rabbit Anti...