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anti-Human p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Reactivity
Human
1760
319
269
99
34
19
13
11
11
10
10
9
5
4
4
4
3
3
3
2
2
2
1
Host
Mouse
1235
612
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
59
49
34
31
23
23
15
15
14
14
14
14
14
14
13
4
4
4
4
4
4
4
4
4
4
3
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1378
582
529
318
314
302
235
156
98
87
53
39
35
17
16
12
12
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN967421
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32227957 ABIN498795 IHC (p) WB Rabbit pSer20 Log in to see Polyclonal
0.32227957 ABIN499003 IHC (p) WB Rabbit Log in to see Polyclonal
0.32227957 ABIN499004 IHC (p) WB Rabbit Log in to see Polyclonal
0.32227957 ABIN498980 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.32227957 ABIN498159 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.32227957 ABIN498887 IHC (p) Rabbit Log in to see Polyclonal
0.32227957 ABIN214388 ICC IF IHC (p) IP ELISA WB Mouse IgG2a, kappa Log in to see
0.32227957 ABIN498796 IHC (p) Rabbit pSer37 Log in to see Polyclonal
0.32227957 ABIN498886 IHC (p) WB Rabbit Log in to see Polyclonal
0.00090133876 ABIN1108553 IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see IMD-53 2
0.00090133876 ABIN120054 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 3
0.00090133876 ABIN358189 EIA IHC (p) WB Rabbit Ig Ser315 Log in to see Polyclonal 5
0.00090133876 ABIN390204 IF IHC (p) WB Rabbit Ig AA 293-322, Ser315 Log in to see Polyclonal 4
0.00090133876 ABIN358762 EIA IHC (p) Rabbit Ig Ser315 Log in to see Polyclonal 5
0.00090133876 ABIN111971 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG2a AA 16-25 Log in to see BP53-12 8
0.00090133876 ABIN120058 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 181-190 Log in to see DO-11 1
0.00090133876 ABIN358188 EIA IHC (p) WB Rabbit Ig pSer20 Log in to see Polyclonal 5
0.00090133876 ABIN358195 EIA IHC (p) WB Rabbit Ig pThr18 Log in to see Polyclonal 5
0.00090133876 ABIN390200 IF IHC (p) WB Rabbit Ig AA 33-62, Thr55 Log in to see Polyclonal 4
0.00090133876 ABIN1687927 IHC (p) Rabbit IgG Log in to see SP5 6

Top referenced anti-p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-p53 Antibodies

Application / Reactivity Human
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 11 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA 550 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 220 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies
Immunocytochemistry (ICC) 82 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 311 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 515 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Reactivity Human
(1760), (319), (269), (99), (34), (19), (13), (11), (11), (10), (10), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1)
Host Mouse
(1235), (612), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(59), (49), (34), (31), (23), (23), (15), (15), (14), (14), (14), (14), (14), (14), (13), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1378), (582), (529), (318), (314), (302), (235), (156), (98), (87), (53), (39), (35), (17), (16), (12), (12), (11), (11), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN967421) Images
Brand BD Pharmingen™
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Recombinant fusion protein
Clone PAb 1801
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN967421) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background The gene for the nuclear phosphoprotein p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is over-expressed in a variety of transformed cells and it forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus and E6 from human papilloma virus. Recent data suggest that wild type p53 plays a role as a checkpoint protein for DNA damage during the S-phase of the cell cycle. However, it is still unclear whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. p53 migrates at a reduced molecular weight of 53 kDa. Clone PAb 1801 recognizes an epitope between amino acids 32-79 in the N-terminal domain of human wild type and mutant p53 antibody. It does not cross-react with p53 from other species. A truncated recombinant human p53 fusion protein was used as immunogen.
Molecular Weight 53 kDa
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN967421) Images back to top
Application Notes Applications include western blot analysis (1-2 µg/ml), immunoprecipitation (1-2 µg/1 x 10^6 cells), immunofluorescence microscopy of cultured cells, immunohistochemistry of frozen (5-20 µg/ml), and antigen-unmasked paraffin-embedded tissue sections (5-20 µg/ml). Positive control cell lines include SK-BR-3 human breast carcinoma cells (ATCC HTB-30), and A431 human vulval carcinoma cells (ATCC CRL-1555). COS-7 SV40 transformed monkey kidney cells (ATCC CRL-1651) or another SV40-transformed cell line are also useful as positive controls for detecting p53. MCF-7 human breast carcinoma cells (ATCC HTB-22) are suggested as a negative control. Positive immunostaining is seen in a high proportion of breast and colon carcinomas. p53 staining is not typically detected in normal skin, brain, kidney, lung, stomach or breast tissue.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN967421) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-p53 Antibody (ABIN967421)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Jacquemier, Molès, Penault-Llorca, Adélaide, Torrente, Viens, Birnbaum, Theillet: "p53 immunohistochemical analysis in breast cancer with four monoclonal antibodies: comparison of staining and PCR-SSCP results." in: British journal of cancer, Vol. 69, Issue 5, pp. 846-52, 1994

Legros, Lacabanne, dAgay, Larsen, Pla, Soussi: "Production of human p53 specific monoclonal antibodies and their use in immunohistochemical studies of tumor cells." in: Bulletin du cancer, Vol. 80, Issue 2, pp. 102-10, 1994

Said, Barrera, Shintaku, Nakamura, Koeffler: "Immunohistochemical analysis of p53 expression in malignant lymphomas." in: The American journal of pathology, Vol. 141, Issue 6, pp. 1343-8, 1993

Vojt?sek, Bártek, Midgley, Lane: "An immunochemical analysis of the human nuclear phosphoprotein p53. New monoclonal antibodies and epitope mapping using recombinant p53." in: Journal of immunological methods, Vol. 151, Issue 1-2, pp. 237-44, 1992

Walker, Dearing, Lane, Varley: "Expression of p53 protein in infiltrating and in-situ breast carcinomas." in: The Journal of pathology, Vol. 165, Issue 3, pp. 203-11, 1992

Vogelstein: "Cancer. A deadly inheritance." in: Nature, Vol. 348, Issue 6303, pp. 681-2, 1991

Baker, Markowitz, Fearon, Willson, Vogelstein: "Suppression of human colorectal carcinoma cell growth by wild-type p53." in: Science (New York, N.Y.), Vol. 249, Issue 4971, pp. 912-5, 1990

Banks, Matlashewski, Crawford: "Isolation of human-p53-specific monoclonal antibodies and their use in the studies of human p53 expression." in: European journal of biochemistry / FEBS, Vol. 159, Issue 3, pp. 529-34, 1986

Background publications

Porter, Gown, Kramp, Coltrera: "Widespread p53 overexpression in human malignant tumors. An immunohistochemical study using methacarn-fixed, embedded tissue." in: The American journal of pathology, Vol. 140, Issue 1, pp. 145-53, 1992

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN967421) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (ABIN967421) Anti-human p53. Formalin-fixed, paraffin-embedded tissue section of breast carcinoma ...
 image for anti-p53 antibody (Tumor Protein P53) (ABIN967421) anti-Tumor Protein P53 (TP53) antibody (Image 2)