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anti-Human p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 50-100
156
103
87
67
65
64
55
52
51
51
46
46
41
39
30
30
27
25
25
22
21
21
19
17
17
13
13
13
13
13
12
11
11
10
10
9
9
8
8
8
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2059
383
317
120
101
89
56
54
29
23
19
15
13
10
10
5
5
4
4
3
3
3
3
2
2
Host
Rabbit
1230
926
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
68
61
49
45
31
31
23
23
17
17
16
16
16
14
14
14
14
14
14
13
13
13
13
13
12
12
6
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
1649
732
558
492
432
418
396
230
192
156
53
38
35
17
16
12
11
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN152309
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.20781031 ABIN967421 IHC (fro) IF IHC (p) IP WB Mouse IgG1 Log in to see PAb 1801 9
0.20781031 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see PAb 1801 7
0.20781031 ABIN152249 ICC FACS IHC (fro) IF IHC (p) IP IHC ELISA WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 19
0.20781031 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
0.20781031 ABIN498795 IHC (p) WB Rabbit pSer20 Log in to see Polyclonal
0.20781031 ABIN498159 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN498887 IHC (p) Rabbit Log in to see Polyclonal
0.20781031 ABIN498980 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN499003 IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN499004 IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal 2
0.20781031 ABIN214388 ICC IF IHC (p) IP ELISA WB Mouse IgG2a, kappa Log in to see
0.20781031 ABIN498796 IHC (p) Rabbit pSer37 Log in to see Polyclonal
0.20781031 ABIN498886 IHC (p) WB Rabbit Log in to see Polyclonal
0.20781031 ABIN4342748 FACS ICC IF IHC IHC (p) SimWes WB Mouse IgG1 Log in to see 5-00E02
0.20781031 ABIN4342749 ICC IF IHC IHC (p) IP WB Rabbit Log in to see Polyclonal
0.00089362357 ABIN1108553 IHC (fro) IF IHC (p) WB Mouse IgG2a Log in to see IMD-53 2
0.00089362357 ABIN358762 EIA IHC (p) Rabbit Ig Ser315 Log in to see Polyclonal 5
0.00089362357 ABIN358189 EIA IHC (p) WB Rabbit Ig Ser315 Log in to see Polyclonal 5
0.00089362357 ABIN120054 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 3

Top referenced anti-p53 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-p53 Antibodies

Application / Reactivity Human
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Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA 681 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 380 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies
Immunocytochemistry (ICC) 187 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 424 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 541 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 50-100
(156), (103), (87), (67), (65), (64), (55), (52), (51), (51), (46), (46), (41), (39), (30), (30), (27), (25), (25), (22), (21), (21), (19), (17), (17), (13), (13), (13), (13), (13), (12), (11), (11), (10), (10), (9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2059), (383), (317), (120), (101), (89), (56), (54), (29), (23), (19), (15), (13), (10), (10), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Rabbit
(1230), (926), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(68), (61), (49), (45), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (12), (12), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(1649), (732), (558), (492), (432), (418), (396), (230), (192), (156), (53), (38), (35), (17), (16), (12), (11), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) Images
Specificity This is specific for human p53.
Cross-Reactivity (Details) The antibody is not likely to react with the mouse ortholog owing to divergence of sequence at the site to which the epitope maps.
Purification Immunogen affinity purified
Immunogen The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
UniProt P04637
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN152309) Images back to top
Application Notes Western Blot 1:5000-1:25000, Flow Cytometry 1.5 μg/10^6 cells in 150 μL, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500, Immunoprecipitation 2 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Proximity Ligation Assay 1:1000-1:20000, Chromatin Immunoprecipitation (ChIP) 4 μg/30 μg
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN152309) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/phosphate buffer, pH 7 to 8
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-p53 Antibody (ABIN152309)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Spurlock, Tossberg, Matlock, Olsen, Aune: "Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 66, Issue 11, pp. 2947-57, 2014

Spurlock, Gass, Bryant, Wells, Olsen, Aune: "Methotrexate-mediated inhibition of nuclear factor κB activation by distinct pathways in T cells and fibroblast-like synoviocytes." in: Rheumatology (Oxford, England), Vol. 54, Issue 1, pp. 178-87, 2014

Spurlock, Tossberg, Fuchs, Olsen, Aune: "Methotrexate increases expression of cell cycle checkpoint genes via JNK activation." in: Arthritis and rheumatism, Vol. 64, Issue 6, pp. 1780-9, 2012 (Sample species: Human). Further details: Flow Cytometry

Shultz, Saito, Najima, Tanaka, Ochi, Tomizawa, Doi, Sone, Suzuki, Fujiwara, Yasukawa, Ishikawa: "Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 29, pp. 13022-7, 2010

Wang, Wang, Chang, Wu, Chao, Kao, Yuan, Lin, Yang, Chan, Li, Hong, Yang: "p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug." in: Nature cell biology, Vol. 11, Issue 6, pp. 694-704, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Tsai, Tseng, Lin: "Two wobble-splicing events affect ING4 protein subnuclear localization and degradation." in: Experimental cell research, Vol. 314, Issue 17, pp. 3130-41, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN152309) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein p53 (TP53) (AA 50-100) antibody
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) anti-Tumor Protein p53 (TP53) (AA 50-100) antibody (Image 2)
Immunoprecipitation (IP) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Chromatin Immunoprecipitation (ChIP): p53 Antibody [ABIN152309] - Chromatin from MCF7...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Western Blot: p53 Antibody [ABIN152309] - Whole cell lysate (50 ug) from HeLa and 293...
Flow Cytometry (FACS) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Flow Cytometry: p53 Antibody [ABIN152309] - Baseline p53 expression in human lymphocy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Immunohistochemistry-Paraffin: p53 Antibody [ABIN152309] - Human skin carcinoma. Anti...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate In Situ PLA...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-p53 antibody (Tumor Protein P53) (AA 50-100) (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...