anti-Human p53 antibody for Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 50-100
173
128
94
84
74
71
70
62
59
55
50
49
48
47
42
38
32
31
29
28
24
22
20
19
17
16
15
15
15
15
15
14
12
11
10
10
10
9
9
8
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2428
519
426
188
127
94
87
84
35
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Rabbit
1371
1170
7
3
1
1
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
55
49
48
34
34
26
26
25
25
25
25
25
24
24
24
23
22
22
21
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
1931
772
750
741
650
636
434
346
316
179
72
41
32
31
24
17
16
15
13
9
9
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN152309
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.04174 ABIN152250 ChIP FACS IHC (fro) IHC (p) IP IHC RIA ELISA WB Mouse IgG1 Log in to see Pab1801 7
24.04174 ABIN2854816 ICC IF IP IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
24.04174 ABIN2855525 ChIP ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
24.04174 ABIN4342749 ICC IF IHC IHC (p) IP WB Rabbit IgG N-Term Log in to see Polyclonal
24.04174 ABIN521044 IP WB Rabbit AA 1-393, full length Log in to see Polyclonal
24.04174 ABIN563227 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C3
1 ABIN967416 IHC (f) IHC (fro) ICS IP WB Mouse IgG1 full length Log in to see G59-12 11
1 ABIN222890 ICC IP IHC WB Mouse IgG1 Ser20 Log in to see C6A5 2
1 ABIN1531939 ELISA IHC IP WB Rabbit IgG AA 1-50, pSer6 Log in to see Polyclonal 1
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 20
1 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
1 ABIN4342746 IHC IP WB Rabbit pSer15 Log in to see Polyclonal 2
1 ABIN2707674 IC IF IP IHC WB Rabbit N-Term, pSer15 Log in to see Polyclonal
1 ABIN2705091 IC IF IP IHC WB Rabbit C-Term, pSer315 Log in to see Polyclonal
1 ABIN2705095 IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN967419 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
1 ABIN967420 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
1 ABIN967457 IHC (f) IHC (fro) IP WB Mouse IgG2a Log in to see DO 8
1 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal 2
1 ABIN967458 IHC (f) IHC (fro) ICS IP WB Mouse IgG2b Log in to see DO 7

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 50-100
(173), (128), (94), (84), (74), (71), (70), (62), (59), (55), (50), (49), (48), (47), (42), (38), (32), (31), (29), (28), (24), (22), (20), (19), (17), (16), (15), (15), (15), (15), (15), (14), (12), (11), (10), (10), (10), (9), (9), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2428), (519), (426), (188), (127), (94), (87), (84), (35), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Rabbit
(1371), (1170), (7), (3), (1), (1), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (55), (49), (48), (34), (34), (26), (26), (25), (25), (25), (25), (25), (24), (24), (24), (23), (22), (22), (21), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA), Western Blotting (WB)
(1931), (772), (750), (741), (650), (636), (434), (346), (316), (179), (72), (41), (32), (31), (24), (17), (16), (15), (13), (9), (9), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN152309) Images
Specificity This is specific for human p53.
Purification Immunogen affinity purified
Immunogen The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 antibody (ABIN152309) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background Gene Symbol: TP53
Gene ID 7157
UniProt P04637
Research Area DNA/RNA, Cell Cycle, Transcription Factors, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 antibody (ABIN152309) Images back to top
Application Notes Western Blot 1:5000-1:25000, Flow Cytometry 1.5 μg/10^6 cells in 150 μL, Immunohistochemistry 1:1000 - 1:5000, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500, Immunoprecipitation 2 - 10 μg/mg lysate, Immunohistochemistry-Paraffin 1:1000 - 1:5000, Proximity Ligation Assay 1:1000-1:20000, Chromatin Immunoprecipitation (ChIP) 4 μg/30 μgImmunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq 4 μg/30 μg chromatin
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 antibody (ABIN152309) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-p53 antibody (ABIN152309)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Spurlock, Tossberg, Matlock, Olsen, Aune: "Methotrexate inhibits NF-κB activity via long intergenic (noncoding) RNA-p21 induction." in: Arthritis & rheumatology (Hoboken, N.J.), Vol. 66, Issue 11, pp. 2947-57, 2014

Spurlock, Gass, Bryant, Wells, Olsen, Aune: "Methotrexate-mediated inhibition of nuclear factor κB activation by distinct pathways in T cells and fibroblast-like synoviocytes." in: Rheumatology (Oxford, England), Vol. 54, Issue 1, pp. 178-87, 2014

Spurlock, Tossberg, Fuchs, Olsen, Aune: "Methotrexate increases expression of cell cycle checkpoint genes via JNK activation." in: Arthritis and rheumatism, Vol. 64, Issue 6, pp. 1780-9, 2012 (Sample species: Human). Further details: Flow Cytometry

Shultz, Saito, Najima, Tanaka, Ochi, Tomizawa, Doi, Sone, Suzuki, Fujiwara, Yasukawa, Ishikawa: "Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD/SCID/IL2r gamma(null) humanized mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 29, pp. 13022-7, 2010

Wang, Wang, Chang, Wu, Chao, Kao, Yuan, Lin, Yang, Chan, Li, Hong, Yang: "p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug." in: Nature cell biology, Vol. 11, Issue 6, pp. 694-704, 2009

Shao, Fujii, Colmegna, Oishi, Goronzy, Weyand: "Deficiency of the DNA repair enzyme ATM in rheumatoid arthritis." in: The Journal of experimental medicine, Vol. 206, Issue 6, pp. 1435-49, 2009

Tsai, Tseng, Lin: "Two wobble-splicing events affect ING4 protein subnuclear localization and degradation." in: Experimental cell research, Vol. 314, Issue 17, pp. 3130-41, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN152309) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) anti-Tumor Protein P53 (TP53) (AA 50-100) antibody
Flow Cytometry (FACS) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (Image 2)
Immunoprecipitation (IP) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Chromatin Immunoprecipitation (ChIP): p53 Antibody [ABIN152309] - Chromatin from MCF7...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Western Blot: p53 Antibody [ABIN152309] - Whole cell lysate (50 ug) from HeLa and 293...
Flow Cytometry (FACS) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Flow Cytometry: p53 Antibody [ABIN152309] - Baseline p53 expression in human lymphocy...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Immunohistochemistry-Paraffin: p53 Antibody [ABIN152309] - Human skin carcinoma. Anti...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate In Situ PLA...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Proximity Ligation Assay (PLA) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Proximity Ligation Assay: p53 Antibody [ABIN152309] - Secondary-conjugate Duolink II ...
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (AA 50-100) antibody (ABIN152309) Western Blot: p53 Antibody - Detection of Human p53 by Western Blot. Samples: Whole ...