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anti-Human p53 antibody for Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
N-Term
164
109
89
64
64
63
54
51
51
50
48
46
41
39
31
30
27
26
25
22
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
9
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
2048
381
310
124
102
93
56
54
27
23
19
16
13
11
10
10
5
4
4
3
3
2
2
1
Host
Rabbit
1240
916
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1657
717
558
487
436
427
397
237
195
156
53
39
35
17
16
13
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN2854816
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.51226807 ABIN2855525 ChIP ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
0.51226807 ABIN521044 IP WB Rabbit AA 1-393, full length Log in to see Polyclonal
0.51226807 ABIN563227 IF IHC (p) IP ELISA WB Mouse IgG1 kappa AA 94-201, partial Log in to see 2C3
0.0008941957 ABIN967416 IHC (f) IHC (fro) ICS IP WB Mouse IgG1 full length Log in to see G59-12 11
0.0008941957 ABIN222890 ICC IP IHC WB Mouse IgG1 Ser20 Log in to see C6A5 2
0.0008941957 ABIN120054 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 3
0.0008941957 ABIN863095 FACS IP IHC RIA ELISA WB Mouse AA 46-53 Log in to see Pab 1801 4
0.0008941957 ABIN111971 EIA IHC (fro) IF IHC (p) IP WB Mouse IgG2a AA 16-25 Log in to see BP53-12 8
0.0008941957 ABIN120058 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 181-190 Log in to see DO-11 1
0.0008941957 ABIN264401 EIA IP WB Mouse IgG1 AA 16-25 Log in to see X77 2
0.0008941957 ABIN320175 EIA IHC (fro) IHC (p) IP WB Mouse IgG2a AA 20-25 Log in to see DO-1 4
0.0008941957 ABIN967420 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
0.0008941957 ABIN967419 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 14-289 Log in to see PAb 240 7
0.0008941957 ABIN967409 FACS IF IP WB Mouse IgG2b Log in to see PAb 122 5
0.0008941957 ABIN967457 IHC (f) IHC (fro) IP WB Mouse IgG2a Log in to see DO 8
0.0008941957 ABIN967458 IHC (f) IHC (fro) ICS IP WB Mouse IgG2b Log in to see DO 7
0.0008941957 ABIN967802 IF IP IHC WB Mouse IgG2b, kappa AA 195-393 Log in to see 80-p53 5
0.0008941957 ABIN967803 IF IP IHC WB Mouse IgG2b, kappa AA 195-393 Log in to see 80-p53 5
0.0008941957 ABIN967421 IHC (fro) IF IHC (p) IP WB Mouse IgG1 Log in to see PAb 1801 9
0.0008941957 ABIN967422 IHC (f) IHC (fro) IF IP WB Mouse IgG1 Log in to see PAb 1801 9

Top referenced anti-p53 antibody for Immunoprecipitation

Similar anti-p53 Antibodies

Application / Reactivity Human
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA 665 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Enzyme Immunoassay (EIA) 17 Antibodies
Flow Cytometry (FACS) 382 Antibodies
Gel Shift (GS) 3 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 35 Antibodies
Immunocytochemistry (ICC) 191 Antibodies
Immunodiffusion (ID) 16 Antibodies
Immunofluorescence (IF) 429 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 156 Antibodies
Immunohistochemistry (IHC) 541 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 11 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope N-Term
(164), (109), (89), (64), (64), (63), (54), (51), (51), (50), (48), (46), (41), (39), (31), (30), (27), (26), (25), (22), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(2048), (381), (310), (124), (102), (93), (56), (54), (27), (23), (19), (16), (13), (11), (10), (10), (5), (4), (4), (3), (3), (2), (2), (1)
Host Rabbit
(1240), (916), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1657), (717), (558), (487), (436), (427), (397), (237), (195), (156), (53), (39), (35), (17), (16), (13), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (92 %)
Characteristics Rabbit Polyclonal antibody to p53 (tumor protein p53)
p53 antibody [N1], N-term
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human p53. The exact sequence is proprietary.
Isotype IgG

Target Details p53

Product Details anti-p53 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Cellular Localization: Cytoplasm , Nucleus , Endoplasmic reticulum
Molecular Weight 44 kDa
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: HCT116 24hr cisplatin 30uM

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2854816) ICC/IF Image p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluore...
Immunoprecipitation (IP) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2854816) IP Image Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treat...
Immunohistochemistry (IHC) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2854816) IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using p...
Immunofluorescence (IF) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2854816) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...