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anti-Mouse (Murine) p53 antibody for Enzyme Immunoassay

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
pThr18
170
118
84
78
68
64
54
54
53
51
49
45
45
43
41
32
30
29
24
23
23
21
20
19
17
17
15
15
15
15
15
14
11
10
9
9
9
9
8
8
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
2252
508
416
186
127
94
85
82
34
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
Host
Rabbit
1275
1080
7
3
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
77
74
62
52
45
44
30
30
24
24
24
24
24
24
24
23
23
23
21
20
19
18
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
3
3
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1781
715
715
663
602
571
399
340
279
179
54
41
32
31
18
16
16
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN358195
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN264401 EIA IP WB Mouse IgG1 AA 16-25 Log in to see X77 2
1 ABIN264449 EIA IHC (fro) IP WB Mouse IgG2b AA 371-380 Log in to see HR231

Top referenced anti-p53 antibody for Enzyme Immunoassay

Similar anti-p53 Antibodies

Application / Reactivity Mouse (Murine)
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 6 Antibodies
Dot Blot (DB) 7 Antibodies
ELISA 162 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 112 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 94 Antibodies
Immunofluorescence (IF) 149 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 76 Antibodies
Immunohistochemistry (IHC) 132 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 112 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 133 Antibodies
Immunoprecipitation (IP) 87 Antibodies
Intracellular Staining (ICS) 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope pThr18
(170), (118), (84), (78), (68), (64), (54), (54), (53), (51), (49), (45), (45), (43), (41), (32), (30), (29), (24), (23), (23), (21), (20), (19), (17), (17), (15), (15), (15), (15), (15), (14), (11), (10), (9), (9), (9), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(2252), (508), (416), (186), (127), (94), (85), (82), (34), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1)
Host Rabbit
(1275), (1080), (7), (3), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(77), (74), (62), (52), (45), (44), (30), (30), (24), (24), (24), (24), (24), (24), (24), (23), (23), (23), (21), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1781), (715), (715), (663), (602), (571), (399), (340), (279), (179), (54), (41), (32), (31), (18), (16), (16), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN358195) Images
Specificity This antibody detects p53 pThr18. Predicted to cross react with Rabbit (100 % Antigen Homology).
Purification Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T18 of human p53.
Isotype Ig

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN358195) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Molecular Weight 43653 Da
Gene ID 7157, 9606
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN358195) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN358195) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-p53 Antibody (ABIN358195)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Seemann, Hainaut: "Roles of thioredoxin reductase 1 and APE/Ref-1 in the control of basal p53 stability and activity." in: Oncogene, Vol. 24, Issue 24, pp. 3853-63, 2005

Mann, Hainaut: "Aminothiol WR1065 induces differential gene expression in the presence of wild-type p53." in: Oncogene, Vol. 24, Issue 24, pp. 3964-75, 2005

Garden, Morrison: "The multiple roles of p53 in the pathogenesis of HIV associated dementia." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 799-809, 2005

Castedo, Perfettini, Piacentini, Kroemer: "p53-A pro-apoptotic signal transducer involved in AIDS." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 701-6, 2005

Duensing, Duensing: "Guilt by association? p53 and the development of aneuploidy in cancer." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 694-700, 2005

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN358195) back to top
Supplier Images
 image for anti-p53 antibody (Tumor Protein P53) (pThr18) (ABIN358195) anti-Tumor Protein P53 (TP53) (pThr18) antibody
 image for anti-p53 antibody (Tumor Protein P53) (pThr18) (ABIN358195) anti-Tumor Protein P53 (TP53) (pThr18) antibody (Image 2)