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anti-Mouse (Murine) p53 antibody for Enzyme Immunoassay

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
pThr18
123
87
62
54
51
49
49
48
46
46
43
40
39
30
27
25
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
8
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
1760
318
269
99
34
19
13
11
11
10
10
9
5
4
4
4
3
3
3
2
2
2
1
Host
Rabbit
1234
613
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
59
49
34
31
23
23
15
15
14
14
14
14
14
14
13
4
4
4
4
4
4
4
4
4
4
3
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1378
582
529
319
315
302
235
156
99
87
53
39
35
16
16
12
12
11
11
9
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN358195
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN264401 EIA IP WB Mouse IgG1 AA 16-25 Log in to see X77 2
0.0008833851 ABIN264449 EIA IHC (fro) IP WB Mouse IgG2b AA 371-380 Log in to see HR231

Top referenced anti-p53 antibody for Enzyme Immunoassay

Similar anti-p53 Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 11 Antibodies
ELISA 73 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 58 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 74 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 45 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Western Blotting (WB) 222 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope pThr18
(123), (87), (62), (54), (51), (49), (49), (48), (46), (46), (43), (40), (39), (30), (27), (25), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (8), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(1760), (318), (269), (99), (34), (19), (13), (11), (11), (10), (10), (9), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1)
Host Rabbit
(1234), (613), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(59), (49), (34), (31), (23), (23), (15), (15), (14), (14), (14), (14), (14), (14), (13), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1378), (582), (529), (319), (315), (302), (235), (156), (99), (87), (53), (39), (35), (16), (16), (12), (12), (11), (11), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN358195) Images
Specificity This antibody detects p53 pThr18. Predicted to cross react with Rabbit (100 % Antigen Homology).
Purification Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T18 of human p53.
Isotype Ig

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN358195) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Molecular Weight 43653 Da
Gene ID 7157, 9606
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN358195) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN358195) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-p53 Antibody (ABIN358195)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Seemann, Hainaut: "Roles of thioredoxin reductase 1 and APE/Ref-1 in the control of basal p53 stability and activity." in: Oncogene, Vol. 24, Issue 24, pp. 3853-63, 2005

Mann, Hainaut: "Aminothiol WR1065 induces differential gene expression in the presence of wild-type p53." in: Oncogene, Vol. 24, Issue 24, pp. 3964-75, 2005

Garden, Morrison: "The multiple roles of p53 in the pathogenesis of HIV associated dementia." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 799-809, 2005

Castedo, Perfettini, Piacentini, Kroemer: "p53-A pro-apoptotic signal transducer involved in AIDS." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 701-6, 2005

Duensing, Duensing: "Guilt by association? p53 and the development of aneuploidy in cancer." in: Biochemical and biophysical research communications, Vol. 331, Issue 3, pp. 694-700, 2005

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN358195) back to top
Supplier Images
 image for anti-p53 antibody (Tumor Protein P53) (pThr18) (ABIN358195) anti-Tumor Protein P53 (TP53) (pThr18) antibody
 image for anti-p53 antibody (Tumor Protein P53) (pThr18) (ABIN358195) anti-Tumor Protein P53 (TP53) (pThr18) antibody (Image 2)