anti-Mouse (Murine) p53 antibody for Immunocytochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • BCC7
  • LFS1
  • P53
  • TRP53
  • p53
  • Trp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • tp53
  • Xp53
  • TP53
  • tumor protein p53
  • cellular tumor antigen p53
  • transformation related protein 53
  • tumor protein p53 L homeolog
  • transformation related protein 53, pseudogene
  • Wistar clone pR53P1 p53 pseudogene
  • TP53
  • CpipJ_CPIJ002758
  • Tp53
  • tp53
  • Trp53
  • tp53.L
  • Trp53-ps
  • p53-ps
Epitope
Ser20
173
128
90
84
73
71
70
61
58
54
49
48
48
47
41
38
32
30
28
24
22
20
17
16
15
15
15
15
15
15
14
12
11
11
10
10
10
9
9
8
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2381
514
425
188
127
94
87
84
35
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1353
1141
7
3
1
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
54
46
45
31
31
26
25
25
25
25
25
25
24
24
24
22
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1909
771
741
741
631
624
434
344
315
179
72
41
32
31
21
17
17
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN222890
$ 310.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 20
1 ABIN4342783 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12 1
1 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal 2
1 ABIN1740217 ELISA ICC IF IHC (p) Rabbit IgG acLys379 Log in to see Polyclonal
1 ABIN1078634 ICC IHC IP WB Rabbit IgG AA 137-390 Log in to see Polyclonal
1 ABIN4342781 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342782 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342741 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
1 ABIN4342748 FACS ICC IF IHC IHC (p) SimWes WB Mouse IgG1 Log in to see 5E2
1 ABIN4342800 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342799 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342803 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342804 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342802 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342797 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342805 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342811 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342812 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342798 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12
1 ABIN4342808 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2a AA 16-25, N-Term Log in to see BP53-12

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Ser20
(173), (128), (90), (84), (73), (71), (70), (61), (58), (54), (49), (48), (48), (47), (41), (38), (32), (30), (28), (24), (22), (20), (17), (16), (15), (15), (15), (15), (15), (15), (14), (12), (11), (11), (10), (10), (10), (9), (9), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2381), (514), (425), (188), (127), (94), (87), (84), (35), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1353), (1141), (7), (3), (1), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (54), (46), (45), (31), (31), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (22), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1909), (771), (741), (741), (631), (624), (434), (344), (315), (179), (72), (41), (32), (31), (21), (17), (17), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN222890) Images
Blocking Peptide Blocking peptide for this product available: ABIN412862
Purification Affinity purified
Immunogen Synthetic peptide corresponding to residue surrounding Ser20 of human p53
Clone C6A5
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 antibody (ABIN222890) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Synonyms: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 antibody (ABIN222890) Images back to top
Application Notes Western blotting (0.5-4 μg/mL), immunoprecipitation (4-8 μg/mL), Immunohistochemistry (4-8 μg/mL), immunocytochemistry (4-8 μg/mL). However, the optimal conditions should be determined individually.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 antibody (ABIN222890) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) in PBS containing 1 mg/mL BSA and 1.5 mM sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-p53 antibody (ABIN222890)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Javed, Khan, Khan, Vaibhav, Ahmad, Khuwaja, Ahmed, Raza, Ashafaq, Tabassum, Siddiqui, El-Agnaf, Safhi, Islam: "S-allyl cysteine attenuates oxidative stress associated cognitive impairment and neurodegeneration in mouse model of streptozotocin-induced experimental dementia of Alzheimer's type." in: Brain research, Vol. 1389, pp. 133-42, 2011

Khan, Ishrat, Ahmad, Hoda, Khan, Khuwaja, Srivastava, Raza, Islam, Ahmad: "Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 255-63, 2009

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 antibody (ABIN222890) back to top
Supplier Images
Western Blotting (WB) image for anti-Tumor Protein P53 (TP53) (Ser20) antibody (ABIN222890) Western blot analysis of p53 in Cos cell lysate.