You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Pig (Porcine) p53 antibody for Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
Mutant, AA 213-217
164
110
89
64
64
63
54
51
51
50
48
46
41
39
31
30
27
26
25
22
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
9
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
2048
380
309
123
101
93
55
54
26
23
18
16
13
11
10
10
5
4
4
3
3
2
2
1
Host
Mouse
1241
915
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
1657
717
558
488
437
427
397
237
196
156
53
39
35
17
16
13
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN187565
$ 669.53
Plus shipping costs $45.00

Top referenced anti-p53 antibody for Immunoprecipitation

Similar anti-p53 Antibodies

Application / Reactivity Human Cow (Bovine) Mouse (Murine) Rat (Rattus) Pig (Porcine) Chicken Hamster Monkey
Western Blotting (WB) 1572 Antibodies 87 Antibodies 282 Antibodies 224 Antibodies 15 Antibodies 26 Antibodies 52 Antibodies 109 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 54 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Mutant, AA 213-217
(164), (110), (89), (64), (64), (63), (54), (51), (51), (50), (48), (46), (41), (39), (31), (30), (27), (26), (25), (22), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(2048), (380), (309), (123), (101), (93), (55), (54), (26), (23), (18), (16), (13), (11), (10), (10), (5), (4), (4), (3), (3), (2), (2), (1)
Host Mouse
(1241), (915), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(1657), (717), (558), (488), (437), (427), (397), (237), (196), (156), (53), (39), (35), (17), (16), (13), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN187565) Images
Specificity Reacts specifically with p53 oncoprotein. Reacts only with mutant p53 under non-denaturing conditions (immunoprecipitation). Reacts with both mutant and wild-type p53 under denatured conditions (Western blot). Species Reactivity: Human. Others not known. NOTE: Previous lots have been reported by end-users to react with mouse, rat, chicken, monkey, bovine, pig, and hamster. Molecular Weight: .53 kDa Cellular Location: Nuclear.Positive Control: MDA-231 or SKBR3 cells. Colon carcinoma.
Immunogen Murine p53 protein containing aa 14-289
Clone Pab 240
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN187565) Images back to top
Antigen
Alternative Name P53 (TP53) (TP53 Antibody Abstract)
Background P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNA damage and possibly facilitates repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN187565) Images back to top
Application Notes Flow Cytometry Immunohistochemistry: Use at 2-4 µg/mL for 30 min. at RT. (methacarn or formalin/paraffin) Recommend for murine tissues only. [Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min. followed by cooling at RT for 20 min.] Immunoprecipitation: (Native & Denatured) Use 2 µg/mg protein lysate. Use Protein G. Western blot: Use at 1-2 µg/mL for 2 hrs at RT. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN187565) Images back to top
Format Liquid
Buffer 500 µL of 200 µg/mL monoclonal antibody in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-p53 Antibody (ABIN187565)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Eeles, Bartkova, Lane, Bartek: "The role of TP53 in breast cancer development." in: Cancer surveys, Vol. 18, pp. 57-75, 1994

Gannon, Greaves, Iggo, Lane: "Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form." in: The EMBO journal, Vol. 9, Issue 5, pp. 1595-602, 1990