anti-Pig (Porcine) p53 antibody for Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
Mutant, AA 213-217
175
125
91
84
74
71
70
62
59
55
50
49
48
45
42
38
32
31
28
24
22
20
17
16
15
15
15
15
15
15
14
11
10
10
10
9
9
8
8
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
2387
516
423
185
126
94
84
82
34
22
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
1
Host
Mouse
1365
1133
7
3
3
1
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
81
78
65
54
46
45
31
31
26
25
25
25
25
25
25
24
24
24
22
20
19
18
14
14
14
14
14
12
7
5
5
5
5
5
5
5
5
4
3
2
2
2
Application
Immunoprecipitation (IP), Western Blotting (WB)
1915
768
747
733
626
615
431
346
314
179
72
41
32
31
21
17
17
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN187565
$ 722.33
Plus shipping costs $45.00

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Mutant, AA 213-217
(175), (125), (91), (84), (74), (71), (70), (62), (59), (55), (50), (49), (48), (45), (42), (38), (32), (31), (28), (24), (22), (20), (17), (16), (15), (15), (15), (15), (15), (15), (14), (11), (10), (10), (10), (9), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(2387), (516), (423), (185), (126), (94), (84), (82), (34), (22), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1)
Host Mouse
(1365), (1133), (7), (3), (3), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(81), (78), (65), (54), (46), (45), (31), (31), (26), (25), (25), (25), (25), (25), (25), (24), (24), (24), (22), (20), (19), (18), (14), (14), (14), (14), (14), (12), (7), (5), (5), (5), (5), (5), (5), (5), (5), (4), (3), (2), (2), (2)
Application Immunoprecipitation (IP), Western Blotting (WB)
(1915), (768), (747), (733), (626), (615), (431), (346), (314), (179), (72), (41), (32), (31), (21), (17), (17), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN187565) Images
Specificity Reacts specifically with p53 oncoprotein. Reacts only with mutant p53 under non-denaturing conditions (immunoprecipitation). Reacts with both mutant and wild-type p53 under denatured conditions (Western blot). Species Reactivity: Human. Others not known. NOTE: Previous lots have been reported by end-users to react with mouse, rat, chicken, monkey, bovine, pig, and hamster. Molecular Weight: .53 kDa Cellular Location: Nuclear.Positive Control: MDA-231 or SKBR3 cells. Colon carcinoma.
Immunogen Murine p53 protein containing aa 14-289
Clone Pab 240
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling ProductDetails: References for anti-p53 antibody (ABIN187565) Images back to top
Antigen
Alternative Name P53 (TP53) (TP53 Antibody Abstract)
Background P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNA damage and possibly facilitates repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling ProductDetails: References for anti-p53 antibody (ABIN187565) Images back to top
Application Notes Flow Cytometry Immunohistochemistry: Use at 2-4 µg/mL for 30 min. at RT. (methacarn or formalin/paraffin) Recommend for murine tissues only. [Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min. followed by cooling at RT for 20 min.] Immunoprecipitation: (Native & Denatured) Use 2 µg/mg protein lysate. Use Protein G. Western blot: Use at 1-2 µg/mL for 2 hrs at RT. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details ProductDetails: References for anti-p53 antibody (ABIN187565) Images back to top
Format Liquid
Buffer 500 µL of 200 µg/mL monoclonal antibody in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

ProductDetails: References for anti-p53 antibody (ABIN187565)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Eeles, Bartkova, Lane, Bartek: "The role of TP53 in breast cancer development." in: Cancer surveys, Vol. 18, pp. 57-75, 1994

Gannon, Greaves, Iggo, Lane: "Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form." in: The EMBO journal, Vol. 9, Issue 5, pp. 1595-602, 1990