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anti-Pig (Porcine) p53 antibody for Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
Mutant, AA 213-217
156
103
87
67
66
65
56
52
52
52
47
46
42
40
31
30
28
25
25
22
22
21
20
17
17
14
14
14
14
14
12
11
11
10
10
9
9
8
8
8
7
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
2086
393
327
119
100
89
56
53
28
23
18
15
13
10
10
5
5
4
4
3
3
3
3
2
2
Host
Mouse
1254
930
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
68
61
49
45
31
31
30
23
23
17
17
16
16
14
14
14
14
14
14
13
13
13
13
13
12
12
6
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
1658
732
564
492
442
420
397
230
194
170
53
38
35
17
16
12
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN187565
$ 722.33
Plus shipping costs $45.00

Top referenced anti-p53 antibody for Immunoprecipitation

Similar anti-p53 Antibodies

Application / Reactivity Human Monkey Mouse (Murine) Chicken Hamster Cow (Bovine) Rat (Rattus) Pig (Porcine)
Simple Western (SimWes) 9 Antibodies 4 Antibodies 1 Antibodies 3 Antibodies 3 Antibodies 3 Antibodies
Western Blotting (WB) 1582 Antibodies 83 Antibodies 287 Antibodies 231 Antibodies 15 Antibodies 25 Antibodies 50 Antibodies 102 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 54 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Mutant, AA 213-217
(156), (103), (87), (67), (66), (65), (56), (52), (52), (52), (47), (46), (42), (40), (31), (30), (28), (25), (25), (22), (22), (21), (20), (17), (17), (14), (14), (14), (14), (14), (12), (11), (11), (10), (10), (9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Chicken, Hamster, Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(2086), (393), (327), (119), (100), (89), (56), (53), (28), (23), (18), (15), (13), (10), (10), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Mouse
(1254), (930), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(68), (61), (49), (45), (31), (31), (30), (23), (23), (17), (17), (16), (16), (14), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (12), (12), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(1658), (732), (564), (492), (442), (420), (397), (230), (194), (170), (53), (38), (35), (17), (16), (12), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN187565) Images
Specificity Reacts specifically with p53 oncoprotein. Reacts only with mutant p53 under non-denaturing conditions (immunoprecipitation). Reacts with both mutant and wild-type p53 under denatured conditions (Western blot). Species Reactivity: Human. Others not known. NOTE: Previous lots have been reported by end-users to react with mouse, rat, chicken, monkey, bovine, pig, and hamster. Molecular Weight: .53 kDa Cellular Location: Nuclear.Positive Control: MDA-231 or SKBR3 cells. Colon carcinoma.
Immunogen Murine p53 protein containing aa 14-289
Clone Pab 240
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN187565) Images back to top
Antigen
Alternative Name P53 (TP53) (TP53 Antibody Abstract)
Background P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein. p53 senses DNA damage and possibly facilitates repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN187565) Images back to top
Application Notes Flow Cytometry Immunohistochemistry: Use at 2-4 µg/mL for 30 min. at RT. (methacarn or formalin/paraffin) Recommend for murine tissues only. [Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0 for 10-20 min. followed by cooling at RT for 20 min.] Immunoprecipitation: (Native & Denatured) Use 2 µg/mg protein lysate. Use Protein G. Western blot: Use at 1-2 µg/mL for 2 hrs at RT. The optimal dilution for a specific application should be determined by the researcher.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN187565) Images back to top
Format Liquid
Buffer 500 µL of 200 µg/mL monoclonal antibody in 10 mM PBS, pH 7.4, with protein stabilizer and 0.09% sodium azide. Purified from ascites fluid by Protein G chromatography.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-p53 Antibody (ABIN187565)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Eeles, Bartkova, Lane, Bartek: "The role of TP53 in breast cancer development." in: Cancer surveys, Vol. 18, pp. 57-75, 1994

Gannon, Greaves, Iggo, Lane: "Activating mutations in p53 produce a common conformational effect. A monoclonal antibody specific for the mutant form." in: The EMBO journal, Vol. 9, Issue 5, pp. 1595-602, 1990