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anti-Primate p53 antibody for Immunoprecipitation

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
AA 16-25
152
98
84
68
64
64
54
54
54
51
49
45
43
41
31
30
29
28
24
23
23
21
20
19
17
17
15
15
15
15
15
14
11
10
9
9
9
8
8
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Primate
2130
478
399
169
127
93
68
65
34
23
20
18
17
16
15
14
6
4
4
4
4
3
3
3
2
2
1
Host
Mouse
1252
967
7
3
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
71
68
56
47
36
35
24
24
21
21
19
18
18
18
18
17
17
17
17
16
16
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
3
3
2
2
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1717
715
636
579
526
465
398
265
250
179
54
41
32
31
18
16
16
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN111971
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN205508 ICC IHC (p) IP ELISA WB Mouse IgG2a AA 20-25 Log in to see BP53-12
1 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342884 ELISA FACS ICC IF IHC (fro) IP WB PerCP Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342817 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342819 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342820 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342821 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342846 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342822 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342818 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN1104765 ICC IHC (p) IP ELISA WB Mouse IgG2a AA 16-25 Log in to see BP53-12
1 ABIN600049 ICC IHC (p) IP ELISA WB Mouse IgG2a Log in to see BP53-12
1 ABIN4342823 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342830 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7
1 ABIN4342844 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB HRP Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342829 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7
1 ABIN4342827 CyTOF FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
1 ABIN4342815 FACS ICC IF IHC (fro) IHC (p) IP SimWes WB Mouse IgG2b kappa AA 37-45 Log in to see DO-7
1 ABIN4342824 CyTOF ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
1 ABIN4342828 CyTOF FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7

Top referenced anti-p53 antibody for Immunoprecipitation

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Application / Reactivity Primate Human
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ELISA 53 Antibodies 665 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 77 Antibodies
Immunocytochemistry (ICC) 74 Antibodies
Immunofluorescence (IF) 74 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 75 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 62 Antibodies
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Western Blotting (WB) 81 Antibodies
Affinity Purification (AP) 2 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Cellular Assay (CA) 1 Antibodies
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Dot Blot (DB) 27 Antibodies
ELISA (Capture) 2 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 16-25
(152), (98), (84), (68), (64), (64), (54), (54), (54), (51), (49), (45), (43), (41), (31), (30), (29), (28), (24), (23), (23), (21), (20), (19), (17), (17), (15), (15), (15), (15), (15), (14), (11), (10), (9), (9), (9), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Primate
(2130), (478), (399), (169), (127), (93), (68), (65), (34), (23), (20), (18), (17), (16), (15), (14), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1)
Host Mouse
(1252), (967), (7), (3), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(71), (68), (56), (47), (36), (35), (24), (24), (21), (21), (19), (18), (18), (18), (18), (17), (17), (17), (17), (16), (16), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1717), (715), (636), (579), (526), (465), (398), (265), (250), (179), (54), (41), (32), (31), (18), (16), (16), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN111971) Images
Specificity This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.
Purification Precipitation Methods
Immunogen Bacterially expressed full-length wild-type p53.
Clone BP53-12
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN111971) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN111971) Images back to top
Application Notes ELISA. Western Blotting (Non-reducing conditions): Recommended Dilution: 1-2 μg/mL, overnight in 4 °CPositive Control: RAMOS Human lymphoma cell lineSample Preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer(1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. SDS-PAGE: 12 % separating gel. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Frozen and Paraffin-Embedded Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN111971) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-p53 Antibody (ABIN111971)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Tanigawa, Fujii, Hou: "Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells." in: Bioscience, biotechnology, and biochemistry, Vol. 72, Issue 3, pp. 797-804, 2008

Taylor, DePrimo, Agarwal, Agarwal, Schönthal, Katula, Stark: "Mechanisms of G2 arrest in response to overexpression of p53." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3607-22, 1999

Agarwal, Agarwal, Taylor, Chernova, Sharma, Stark: "A p53-dependent S-phase checkpoint helps to protect cells from DNA damage in response to starvation for pyrimidine nucleotides." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 25, pp. 14775-80, 1999

Taylor, Agarwal, Agarwal, Stacey, Stark: "p53 inhibits entry into mitosis when DNA synthesis is blocked." in: Oncogene, Vol. 18, Issue 2, pp. 283-95, 1999

Agarwal, Agarwal, Taylor, Stark: "p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 18, pp. 8493-7, 1995

Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, 1991

Product cited in:

Dolezalová, Vojt?sek, Kovarík: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, 1997

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Rejthar, Kovarík, Midgley, Lane: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, 1991