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anti-Primate p53 antibody for Western Blotting

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
AA 16-25
164
110
89
64
64
63
54
51
51
50
48
46
41
39
30
30
27
26
25
22
22
20
19
17
17
13
13
13
13
13
12
12
9
9
9
9
8
8
8
8
8
8
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Primate
2048
381
310
124
102
92
56
54
27
23
19
16
13
11
10
10
5
4
4
3
3
2
2
1
Host
Mouse
1241
915
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
66
61
47
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1657
717
558
487
436
427
397
236
196
156
53
39
35
16
16
13
12
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN111971
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011728023 ABIN4342915 WB PerCP Mouse IgG2b kappa AA 37-45 Log in to see DO-7
0.0011728023 ABIN4342916 WB PE Mouse IgG2b kappa AA 37-45 Log in to see DO-7
0.0011728023 ABIN4342917 WB DyLight 350 Mouse IgG2b kappa AA 37-45 Log in to see DO-7
0.0011728023 ABIN4342918 WB DyLight 405 Mouse IgG2b kappa AA 37-45 Log in to see DO-7
0.0011728023 ABIN4342919 WB DyLight 405LS Mouse IgG2b kappa AA 37-45 Log in to see DO-7
0.0011728023 ABIN4342792 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0011728023 ABIN4342793 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0011728023 ABIN4342794 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0011728023 ABIN4342817 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0011728023 ABIN4342818 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0011728023 ABIN4342819 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG AA 20-25 Log in to see BP53-12-DO-7
0.0011728023 ABIN4342820 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342821 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342822 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342835 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342836 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342837 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342838 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342839 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG2b kappa AA 37-45 Log in to see SPM590
0.0011728023 ABIN4342840 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG2b kappa AA 37-45 Log in to see SPM590

Top referenced anti-p53 antibody for Western Blotting

Similar anti-p53 Antibodies

Application / Reactivity Primate Human
ELISA 43 Antibodies 665 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 17 Antibodies
Immunocytochemistry (ICC) 54 Antibodies
Immunofluorescence (IF) 56 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 55 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies
Immunoprecipitation (IP) 52 Antibodies
Western Blotting (WB) 89 Antibodies
Affinity Purification (AP) 2 Antibodies
Cellular Assay (CA) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 12 Antibodies
Dot Blot (DB) 31 Antibodies
ELISA (Capture) 2 Antibodies
ELISA (Detection) 2 Antibodies
Flow Cytometry (FACS) 382 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope AA 16-25
(164), (110), (89), (64), (64), (63), (54), (51), (51), (50), (48), (46), (41), (39), (30), (30), (27), (26), (25), (22), (22), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (12), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Primate
(2048), (381), (310), (124), (102), (92), (56), (54), (27), (23), (19), (16), (13), (11), (10), (10), (5), (4), (4), (3), (3), (2), (2), (1)
Host Mouse
(1241), (915), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(66), (61), (47), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1657), (717), (558), (487), (436), (427), (397), (236), (196), (156), (53), (39), (35), (16), (16), (13), (12), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN111971) Images
Specificity This Monoclonal antibody BP53-12 recognizes defined epitope (aa 16-25) on Human p53, a 50 kDa tumour suppressor found in increased amounts in a wide variety of transformed cells. It is frequently mutated or inactivated in many types of cancer.
Purification Precipitation Methods
Immunogen Bacterially expressed full-length wild-type p53.
Clone BP53-12
Isotype IgG2a

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN111971) Images back to top
Antigen
Alternative Name p53 (TP53) (TP53 Antibody Abstract)
Background The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
Gene ID 7157
NCBI Accession NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN111971) Images back to top
Application Notes ELISA. Western Blotting (Non-reducing conditions): Recommended Dilution: 1-2 μg/mL, overnight in 4 °CPositive Control: RAMOS Human lymphoma cell lineSample Preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer(1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. SDS-PAGE: 12 % separating gel. Immunoprecipitation. Immunocytochemistry. Immunohistochemistry on Frozen and Paraffin-Embedded Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN111971) Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-p53 Antibody (ABIN111971)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Tanigawa, Fujii, Hou: "Stabilization of p53 is involved in quercetin-induced cell cycle arrest and apoptosis in HepG2 cells." in: Bioscience, biotechnology, and biochemistry, Vol. 72, Issue 3, pp. 797-804, 2008

Taylor, DePrimo, Agarwal, Agarwal, Schönthal, Katula, Stark: "Mechanisms of G2 arrest in response to overexpression of p53." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3607-22, 1999

Agarwal, Agarwal, Taylor, Chernova, Sharma, Stark: "A p53-dependent S-phase checkpoint helps to protect cells from DNA damage in response to starvation for pyrimidine nucleotides." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 25, pp. 14775-80, 1999

Taylor, Agarwal, Agarwal, Stacey, Stark: "p53 inhibits entry into mitosis when DNA synthesis is blocked." in: Oncogene, Vol. 18, Issue 2, pp. 283-95, 1999

Agarwal, Agarwal, Taylor, Stark: "p53 controls both the G2/M and the G1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 18, pp. 8493-7, 1995

Bártek, Bártková, Vojt?sek, Stasková, Lukás, Rejthar, Kovarík, Midgley, Gannon, Lane: "Aberrant expression of the p53 oncoprotein is a common feature of a wide spectrum of human malignancies." in: Oncogene, Vol. 6, Issue 9, pp. 1699-703, 1991

Product cited in:

Dolezalová, Vojt?sek, Kovarík: "Epitope analysis of the human p53 tumour suppressor protein." in: Folia biologica, Vol. 43, Issue 1, pp. 49-51, 1997

Bártková, Bártek, Lukás, Vojt?sek, Stasková, Rejthar, Kovarík, Midgley, Lane: "p53 protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia." in: International journal of cancer. Journal international du cancer, Vol. 49, Issue 2, pp. 196-202, 1991