You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rabbit p53 antibody for Western Blotting

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
N-Term
156
104
87
67
67
65
56
54
52
51
46
46
42
40
31
31
28
25
25
23
22
21
19
17
17
13
13
13
13
13
12
11
11
10
10
9
9
8
8
8
8
7
7
7
7
7
7
6
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Pig (Porcine), Rabbit
2129
409
327
121
100
89
57
55
29
23
18
16
14
10
10
5
5
4
4
4
3
3
3
2
2
Host
Rabbit
1287
955
7
4
3
1
1
Clonality
Polyclonal
Conjugate
This p53 antibody is un-conjugated
70
64
51
46
33
32
23
23
18
18
18
17
16
15
15
15
15
15
14
14
14
14
14
14
14
14
7
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
1694
744
619
537
501
448
386
256
250
156
54
39
35
18
17
16
12
12
11
11
9
9
4
4
3
3
2
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2779317
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590793 ABIN2777951 WB Rabbit N-Term Log in to see Polyclonal 1
2.9590793 ABIN2779979 WB Rabbit C-Term Log in to see Polyclonal 1
2.9590793 ABIN2792141 WB Rabbit C-Term Log in to see Polyclonal 1
2.9590793 ABIN2707675 WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN222891 WB Rabbit IgG Log in to see Polyclonal
1 ABIN677408 IF (p) IHC (p) WB Rabbit IgG AA 345-390, Wild Type Log in to see Polyclonal 1
1 ABIN2755790 WB Rabbit N-Term, pSer18 Log in to see Polyclonal
1 ABIN2755791 IC IF IHC WB Rabbit N-Term, pSer20 Log in to see Polyclonal
1 ABIN1002962 WB Rabbit Log in to see Polyclonal
1 ABIN4965470 WB Rabbit IgG Log in to see Polyclonal
1 ABIN3066409 WB Rabbit N-Term, pThr18 Log in to see Polyclonal
1 ABIN3066411 ICC IF IHC WB Rabbit N-Term, pSer20 Log in to see Polyclonal

Top referenced anti-p53 antibody for Western Blotting

Similar anti-p53 Antibodies

Application / Reactivity Human Cow (Bovine) Pig (Porcine) Rabbit
Cytometry by Time of Flight (CyTOF) 18 Antibodies 6 Antibodies
Simple Western (SimWes) 9 Antibodies 3 Antibodies
Western Blotting (WB) 1598 Antibodies 77 Antibodies 15 Antibodies 13 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Radioimmunoassay (RIA) 54 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Intracellular Staining (ICS) 3 Antibodies 1 Antibodies
Intracellular Flow Cytometry (ICFC) 4 Antibodies
Immunostaining (ISt) 1 Antibodies
Immunoprecipitation (IP) 361 Antibodies 26 Antibodies 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope N-Term
(156), (104), (87), (67), (67), (65), (56), (54), (52), (51), (46), (46), (42), (40), (31), (31), (28), (25), (25), (23), (22), (21), (19), (17), (17), (13), (13), (13), (13), (13), (12), (11), (11), (10), (10), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Pig (Porcine), Rabbit
(2129), (409), (327), (121), (100), (89), (57), (55), (29), (23), (18), (16), (14), (10), (10), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2)
Host Rabbit
(1287), (955), (7), (4), (3), (1), (1)
Clonality
Polyclonal
Conjugate This p53 antibody is un-conjugated
(70), (64), (51), (46), (33), (32), (23), (23), (18), (18), (18), (17), (16), (15), (15), (15), (15), (15), (14), (14), (14), (14), (14), (14), (14), (14), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
(1694), (744), (619), (537), (501), (448), (386), (256), (250), (156), (54), (39), (35), (18), (17), (16), (12), (12), (11), (11), (9), (9), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2779317) Images
Blocking Peptide Blocking peptide for this product available: ABIN985382
Sequence EEPQSDPSVE PPLSQETFSD LWKLLPENNV LSPLPSQAMD DLMLSPDDIE
Predicted Reactivity Bovine : 100 %, Human : 100 %, Horse : 90 %
Characteristics This is a rabbit polyclonal antibody against TP53. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TP53

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN2779317) Images back to top
Antigen
Alternative Name TP53 (TP53 Antibody Abstract)
Background TP53 acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.
Molecular Weight 44 kDa
Gene ID 7157
NCBI Accession NM_000546, NP_000537
UniProt P04637
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN2779317) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 393 AA

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN2779317) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-p53 Antibody (ABIN2779317)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Background publications

Boehme, Kulikov, Blattner: "p53 stabilization in response to DNA damage requires Akt/PKB and DNA-PK." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 22, pp. 7785-90, 2008

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN2779317) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) WB Suggested Anti-TP53 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Immunohistochemistry (IHC) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) anti-Tumor Protein P53 (TP53) (N-Term) antibody (Image 2)
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) Host: Rabbit
Target Name: TP53
Sample Tissue: Human 721_B
Antibo...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) Host: Rabbit
Target Name: TP53
Sample Tissue: Human Fetal Liver
...
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (N-Term) (ABIN2779317) Host: Rabbit
Target Name: TP53
Sample Tissue: Human Adult Placenta