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anti-Rat (Rattus) p53 antibody for Immunocytochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • bbl
  • BCC7
  • bfy
  • bhy
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • LFS1
  • p44
  • P53
  • p53
  • tp53
  • TP53
  • Tp53
  • TpMs
  • Trp53
  • TRP53
  • wu:fb40d06
  • Xp53
  • zgc:111919
Epitope
Ser20
160
106
89
64
64
64
55
51
51
51
48
46
41
39
31
30
27
26
25
22
21
20
19
17
17
13
13
13
13
13
12
10
9
9
9
9
8
8
8
8
7
7
7
7
7
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2044
376
312
120
104
93
57
56
30
23
19
16
13
10
10
5
5
4
4
3
3
3
3
2
2
Host
Mouse
1220
921
7
4
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
68
61
49
44
31
31
23
23
17
17
16
16
16
14
14
14
14
14
13
13
13
13
13
13
13
12
12
4
4
4
4
4
4
4
4
4
3
3
1
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1636
721
557
487
436
421
397
234
193
156
53
38
35
17
16
12
11
11
11
9
9
4
3
3
3
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN222890
$ 336.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009990641 ABIN152249 ICC FACS IHC (fro) IF IHC (p) IP IHC ELISA WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 18
0.0009990641 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal 2
0.0009990641 ABIN1740217 ELISA ICC IF IHC (p) Rabbit IgG Lys379, acLys379 Log in to see Polyclonal
0.0009990641 ABIN1002963 ICC IP IHC WB Mouse IgG1 Log in to see C6A5
0.0009990641 ABIN4342785 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.0009990641 ABIN4342786 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.0009990641 ABIN4342784 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.0009990641 ABIN4342787 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.0009990641 ABIN4342788 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB HRP Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
0.0009990641 ABIN4342792 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342794 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342793 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342877 ELISA FACS ICC IF IHC (fro) IP WB Allophycocyanin Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342881 ELISA FACS ICC IF IHC (fro) IP WB DyLight 488 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342879 ELISA FACS ICC IF IHC (fro) IP WB DyLight 650 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342885 ELISA FACS ICC IF IHC (fro) IP WB PE Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342889 ELISA FACS ICC IF IHC (fro) IP WB DyLight 405LS Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342887 ELISA FACS ICC IF IHC (fro) IP WB DyLight 350 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342878 ELISA FACS ICC IF IHC (fro) IP WB Biotin Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
0.0009990641 ABIN4342880 ELISA FACS ICC IF IHC (fro) IP WB DyLight 680 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122

Top referenced anti-p53 antibody for Immunocytochemistry

Similar anti-p53 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 114 Antibodies 126 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 3 Antibodies
Flow Cytometry (FACS) 47 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 45 Antibodies
Immunofluorescence (IF) 80 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 84 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 37 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 58 Antibodies
Immunoprecipitation (IP) 72 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 222 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 10 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Ser20
(160), (106), (89), (64), (64), (64), (55), (51), (51), (51), (48), (46), (41), (39), (31), (30), (27), (26), (25), (22), (21), (20), (19), (17), (17), (13), (13), (13), (13), (13), (12), (10), (9), (9), (9), (9), (8), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2044), (376), (312), (120), (104), (93), (57), (56), (30), (23), (19), (16), (13), (10), (10), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2)
Host Mouse
(1220), (921), (7), (4), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(68), (61), (49), (44), (31), (31), (23), (23), (17), (17), (16), (16), (16), (14), (14), (14), (14), (14), (13), (13), (13), (13), (13), (13), (13), (12), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1636), (721), (557), (487), (436), (421), (397), (234), (193), (156), (53), (38), (35), (17), (16), (12), (11), (11), (11), (9), (9), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN222890) Images
Blocking Peptide Blocking peptide for this product available: ABIN412862
Purification Affinity purified
Immunogen Synthetic peptide corresponding to residue surrounding Ser20 of human p53
Clone C6A5
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN222890) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Synonyms: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN222890) Images back to top
Application Notes Western blotting (0.5-4 μg/mL), Immunoprecipitation (4-8 μg/mL), Immunohistochemistry (4-8 μg/mL), Immunocytochemistry (4-8 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Country of Animal Origin: USA

Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN222890) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) in PBS containing 1 mg/mL BSA and 1.5 mM sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-p53 Antibody (ABIN222890)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Javed, Khan, Khan, Vaibhav, Ahmad, Khuwaja, Ahmed, Raza, Ashafaq, Tabassum, Siddiqui, El-Agnaf, Safhi, Islam: "S-allyl cysteine attenuates oxidative stress associated cognitive impairment and neurodegeneration in mouse model of streptozotocin-induced experimental dementia of Alzheimer's type." in: Brain research, Vol. 1389, pp. 133-42, 2011

Khan, Ishrat, Ahmad, Hoda, Khan, Khuwaja, Srivastava, Raza, Islam, Ahmad: "Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 255-63, 2009

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN222890) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (Ser20) (ABIN222890) Western blot analysis of p53 in Cos cell lysate.