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anti-Rat (Rattus) p53 antibody for Immunocytochemistry

Recommended p53 Antibody (supplied by: Log in to see )

Antigen
Tumor Protein P53 (TP53) Antibodies
  • tp53
  • p53
  • TP53
  • Tp53
  • bbl
  • bfy
  • bhy
  • p44
  • TpMs
  • BCC7
  • LFS1
  • P53
  • TRP53
  • Trp53
  • Xp53
  • brp53
  • drp53
  • etID22686.5
  • fb40d06
  • wu:fb40d06
  • zgc:111919
  • cellular tumor antigen p53
  • tumor protein p53
  • tumor supressor p53
  • p53 tumor suppressor
  • transformation related protein 53
  • transformation related protein 53, pseudogene
  • trichoplein, keratin filament binding
  • Wistar clone pR53P1 p53 pseudogene
  • CpipJ_CPIJ002758
  • TP53
  • tp53
  • P53
  • Trp53
  • Trp53-ps
  • TCHP
  • Tp53
  • p53-ps
Epitope
Ser20
152
99
84
68
64
64
55
54
54
51
49
45
43
41
32
30
29
28
24
23
23
21
20
19
17
17
15
15
15
15
15
14
11
10
9
9
8
8
8
7
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
2131
479
394
169
127
94
68
64
34
23
18
17
16
16
15
11
6
4
4
4
4
3
3
2
2
1
Host
Mouse
1253
968
7
3
3
1
1
Clonality
Monoclonal
Conjugate
This p53 antibody is un-conjugated
71
68
56
47
36
35
24
24
21
21
19
18
18
18
18
17
17
17
17
16
16
15
14
14
14
14
14
12
7
4
4
4
4
4
4
4
4
3
3
2
2
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
1719
716
635
580
527
466
399
264
251
179
55
41
32
31
18
17
15
15
13
9
9
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
Options
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Catalog No. ABIN222890
$ 336.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN152249 CyTOF ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Mouse IgG1 kappa AA 14-389 Log in to see PAb 240 20
1 ABIN4342743 ICC IF IHC IHC (p) IP WB Rabbit pSer15 Log in to see Polyclonal 2
1 ABIN1740217 ELISA ICC IF IHC (p) Rabbit IgG acLys379 Log in to see Polyclonal
1 ABIN1078635 ICC IHC IP WB Rabbit IgG AA 90-354 Log in to see Polyclonal
1 ABIN5078595 FACS ICC IF IHC (fro) Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN5078596 FACS ICC IF IHC (fro) Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN1825640 ICC IP IHC WB Mouse IgG1 Ser20 Log in to see C6A5
1 ABIN4342756 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
1 ABIN4342794 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342758 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
1 ABIN4342785 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 kappa AA 14-389 Log in to see PAb 240
1 ABIN4342793 FACS ICC IF IHC (fro) WB Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342875 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 647 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342874 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 488 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342878 ELISA ICC IF IHC (fro) WB Biotin Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342876 ELISA FACS ICC IF IHC (fro) WB Alexa Fluor 700 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342879 ELISA FACS ICC IF IHC (fro) WB DyLight 650 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342881 ELISA FACS ICC IF IHC (fro) WB DyLight 488 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342880 ELISA FACS ICC IF IHC (fro) WB DyLight 680 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122
1 ABIN4342887 ELISA FACS ICC IF IHC (fro) WB DyLight 350 Mouse IgG2b kappa AA 370-378, C-Term Log in to see PAb122

Top referenced anti-p53 antibody for Immunocytochemistry

Similar anti-p53 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 4 Antibodies
ELISA 127 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 57 Antibodies
Gel Shift (GS) 3 Antibodies
Immunochromatography (IC) 7 Antibodies
Immunocytochemistry (ICC) 51 Antibodies
Immunofluorescence (IF) 103 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 73 Antibodies
Immunohistochemistry (IHC) 88 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 45 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 77 Antibodies
Immunoprecipitation (IP) 67 Antibodies
Intracellular Staining (ICS) 1 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Western Blotting (WB) 291 Antibodies
Chromatin Immunoprecipitation (ChIP) 1 Antibodies

General

Antigen Tumor Protein P53 (TP53) Antibodies
Epitope Ser20
(152), (99), (84), (68), (64), (64), (55), (54), (54), (51), (49), (45), (43), (41), (32), (30), (29), (28), (24), (23), (23), (21), (20), (19), (17), (17), (15), (15), (15), (15), (15), (14), (11), (10), (9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(2131), (479), (394), (169), (127), (94), (68), (64), (34), (23), (18), (17), (16), (16), (15), (11), (6), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Host Mouse
(1253), (968), (7), (3), (3), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This p53 antibody is un-conjugated
(71), (68), (56), (47), (36), (35), (24), (24), (21), (21), (19), (18), (18), (18), (18), (17), (17), (17), (17), (16), (16), (15), (14), (14), (14), (14), (14), (12), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(1719), (716), (635), (580), (527), (466), (399), (264), (251), (179), (55), (41), (32), (31), (18), (17), (15), (15), (13), (9), (9), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-p53 Antibody

Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN222890) Images
Blocking Peptide Blocking peptide for this product available: ABIN412862
Purification Affinity purified
Immunogen Synthetic peptide corresponding to residue surrounding Ser20 of human p53
Clone C6A5
Isotype IgG1

Target Details p53

Product Details anti-p53 Antibody Application Details Handling References for anti-p53 Antibody (ABIN222890) Images back to top
Antigen
Alternative Name p53 (TP53 Antibody Abstract)
Background P53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression throμgh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.
Synonyms: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53
Gene ID 7157
Research Area Cancer, Cell Cycle, Transcription Factors, DNA/RNA, Apoptosis/Necrosis
Pathways p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-p53 Antibody Target Details p53 Handling References for anti-p53 Antibody (ABIN222890) Images back to top
Application Notes Western blotting (0.5-4 μg/mL), immunoprecipitation (4-8 μg/mL), Immunohistochemistry (4-8 μg/mL), immunocytochemistry (4-8 μg/mL). However, the optimal conditions should be determined individually.
Restrictions For Research Use only

Handling

Product Details anti-p53 Antibody Target Details p53 Application Details References for anti-p53 Antibody (ABIN222890) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) in PBS containing 1 mg/mL BSA and 1.5 mM sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

References for anti-p53 Antibody (ABIN222890)

Product Details anti-p53 Antibody Target Details p53 Application Details Handling Images back to top
Product cited in:

Javed, Khan, Khan, Vaibhav, Ahmad, Khuwaja, Ahmed, Raza, Ashafaq, Tabassum, Siddiqui, El-Agnaf, Safhi, Islam: "S-allyl cysteine attenuates oxidative stress associated cognitive impairment and neurodegeneration in mouse model of streptozotocin-induced experimental dementia of Alzheimer's type." in: Brain research, Vol. 1389, pp. 133-42, 2011

Khan, Ishrat, Ahmad, Hoda, Khan, Khuwaja, Srivastava, Raza, Islam, Ahmad: "Sesamin attenuates behavioral, biochemical and histological alterations induced by reversible middle cerebral artery occlusion in the rats." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 255-63, 2009

Images

Product Details anti-p53 Antibody Target Details p53 Application Details Handling References for anti-p53 Antibody (ABIN222890) back to top
Supplier Images
Western Blotting (WB) image for anti-p53 antibody (Tumor Protein P53) (Ser20) (ABIN222890) Western blot analysis of p53 in Cos cell lysate.