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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)|
|Western Blotting (WB)||41 Antibodies||16 Antibodies||7 Antibodies||2 Antibodies|
|Immunoprecipitation (IP)||31 Antibodies||13 Antibodies||7 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||26 Antibodies||12 Antibodies||7 Antibodies||4 Antibodies|
|Immunohistochemistry (IHC)||10 Antibodies||1 Antibodies||2 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies||11 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Antigen||phosphoprotein Associated with Glycosphingolipid Microdomains 1 (PAG1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus), Cow (Bovine) Alternatives|
|Conjugate||This PAG1 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|5 references available|
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Product Details anti-PAG1 AntibodyTarget Details PAG1 Application Details Handling References for anti-PAG1 Antibody (ABIN94270) Images
|Specificity||The antibody PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.|
|Purification||Purified from ascites by protein-A affinity chromatography.|
|Purity||> 95 % (by SDS-PAGE)|
|Immunogen||C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.|
Target Details PAG1Product Details anti-PAG1 Antibody Application Details Handling References for anti-PAG1 Antibody (ABIN94270) Images back to top
|Alternative Name||PAG1 (PAG1 Antibody Abstract)|
|Background||PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.|
|Pathways||p53 Signaling, EGFR Signaling Pathway|
Application DetailsProduct Details anti-PAG1 Antibody Target Details PAG1 Handling References for anti-PAG1 Antibody (ABIN94270) Images back to top
Positive control: RAJI human Burkitt lymphoma cell line
Immunohistochemistry (paraffin sections)
Positive tissue: Appendix (germinal center of lymphatic follicle
Application note: heat-mediated antigen retrieval in citrate buffer pH 6.1
Working concentrations should be determined by the investigator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PAG1 Antibody Target Details PAG1 Application Details References for anti-PAG1 Antibody (ABIN94270) Images back to top
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Do not freeze.|
|Storage Comment||Store at 2-8 °C. Do not use after expiration date stamped on vial label.|
References for anti-PAG1 Antibody (ABIN94270)Product Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling Images back to top
|Product cited in:||
Vang, Abrahamsen, Myklebust et al.: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003 (PubMed).
Davidson, Bakinowski, Thomas et al.: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003 (PubMed).
Simeoni, Smida, Posevitz et al.: "Right time, right place: the organization of membrane proximal signaling." in: Seminars in immunology, Vol. 17, Issue 1, pp. 35-49, 2004 (PubMed).
Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, 2004 (PubMed).
Baumgartner, Angelisová, Setterblad et al.: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003 (PubMed).
ImagesProduct Details anti-PAG1 Antibody Target Details PAG1 Application Details Handling References for anti-PAG1 Antibody (ABIN94270) back to top