You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PML antibody for Enzyme Immunoassay

Recommended PML Antibody (supplied by: Log in to see )

Antigen
Promyelocytic Leukemia (PML) Antibodies
  • 1200009E24Rik
  • AI661194
  • MYL
  • PML
  • PP8675
  • RGD1562602
  • RNF71
  • TRIM19
  • Trim19
Epitope
Sumoylation Site
13
11
9
8
8
7
7
5
3
3
3
2
2
1
1
1
1
1
Reactivity
Human
108
26
8
1
Host
Rabbit
85
40
Clonality
Polyclonal
Conjugate
This PML antibody is un-conjugated
5
5
3
3
3
3
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
104
46
38
37
23
18
13
7
6
3
3
3
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN357734
$ 390.50
Plus shipping costs $45.00

Top referenced anti-PML antibody for Enzyme Immunoassay

Similar anti-PML Antibodies

Application / Reactivity Human
Dot Blot (DB) 3 Antibodies
ELISA 45 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 19 Antibodies
Immunofluorescence (IF) 35 Antibodies
Immunohistochemistry (IHC) 39 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 20 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies
Western Blotting (WB) 91 Antibodies

General

Antigen Promyelocytic Leukemia (PML) Antibodies
Epitope Sumoylation Site
(13), (11), (9), (8), (8), (7), (7), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(108), (26), (8), (1)
Host Rabbit
(85), (40)
Clonality
Polyclonal
Conjugate This PML antibody is un-conjugated
(5), (5), (3), (3), (3), (3)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(104), (46), (38), (37), (23), (18), (13), (7), (6), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-PML Antibody

Target Details PML Application Details Handling References for anti-PML Antibody (ABIN357734) Images
Specificity This antibody is specific to PML (Sumoylation Site).
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PRKVIKMESEEGKEA containing a predicted sumoylation site from the central region of human PML.
Isotype Ig

Target Details PML

Product Details anti-PML Antibody Application Details Handling References for anti-PML Antibody (ABIN357734) Images back to top
Antigen
Alternative Name PML / RNF71 (PML Antibody Abstract)
Background PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL).Synonyms: MYL, Probable transcription factor PML, RING finger protein 71, TRIM19, Tripartite motif-containing protein 19
Molecular Weight 97516 Da
Gene ID 5371, 9606
UniProt P29590
Pathways p53 Signaling, Retinoic Acid Receptor Signaling Pathway

Application Details

Product Details anti-PML Antibody Target Details PML Handling References for anti-PML Antibody (ABIN357734) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PML Antibody Target Details PML Application Details References for anti-PML Antibody (ABIN357734) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PML Antibody (ABIN357734)

Product Details anti-PML Antibody Target Details PML Application Details Handling Images back to top
Background publications

Kawai, Akira, Reed: "ZIP kinase triggers apoptosis from nuclear PML oncogenic domains." in: Molecular and cellular biology, Vol. 23, Issue 17, pp. 6174-86, 2003

Puccetti, Beissert, Güller, Li, Hoelzer, Ottmann, Ruthardt: "Leukemia-associated translocation products able to activate RAS modify PML and render cells sensitive to arsenic-induced apoptosis." in: Oncogene, Vol. 22, Issue 44, pp. 6900-8, 2003

Favre, Butticaz, Stevenson, Jongeneel, Telenti: "High frequency of alternative splicing of human genes participating in the HIV-1 life cycle: a model using TSG101, betaTrCP, PPIA, INI1, NAF1, and PML." in: Journal of acquired immune deficiency syndromes (1999), Vol. 34, Issue 2, pp. 127-33, 2003

Louria-Hayon, Grossman, Sionov, Alsheich, Pandolfi, Haupt: "The promyelocytic leukemia protein protects p53 from Mdm2-mediated inhibition and degradation." in: The Journal of biological chemistry, Vol. 278, Issue 35, pp. 33134-41, 2003

Möller, Sirma, Hofmann, Rueffer, Klimczak, Dröge, Will, Schmitz: "PML is required for homeodomain-interacting protein kinase 2 (HIPK2)-mediated p53 phosphorylation and cell cycle arrest but is dispensable for the formation of HIPK domains." in: Cancer research, Vol. 63, Issue 15, pp. 4310-4, 2003

Images

Product Details anti-PML Antibody Target Details PML Application Details Handling References for anti-PML Antibody (ABIN357734) back to top
Supplier Images
 image for anti-PML antibody (Promyelocytic Leukemia) (Sumoylation Site) (ABIN357734) anti-Promyelocytic Leukemia (PML) (Sumoylation Site) antibody
 image for anti-PML antibody (Promyelocytic Leukemia) (Sumoylation Site) (ABIN357734) anti-Promyelocytic Leukemia (PML) (Sumoylation Site) antibody (Image 2)