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anti-Human PML antibody for Immunocytochemistry

Recommended PML Antibody (supplied by: Log in to see )

Antigen
Promyelocytic Leukemia (PML) Antibodies
  • PML
  • MYL
  • PP8675
  • RNF71
  • TRIM19
  • 1200009E24Rik
  • AI661194
  • Trim19
  • RGD1562602
  • promyelocytic leukemia
  • PML
  • Pml
Epitope
AA 375-425
15
14
8
7
7
6
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
115
28
11
2
2
2
1
1
1
1
Host
Rabbit
93
39
Clonality
Polyclonal
Conjugate
This PML antibody is un-conjugated
5
5
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
115
46
42
40
25
22
16
6
5
5
3
3
2
2
1
1
1
Supplier
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Catalog No. ABIN252967
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN252968 ICC IF IHC IP WB Rabbit AA 575-625 Log in to see Polyclonal 2
1 ABIN4346484 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 3
1 ABIN1175605 ICC IHC IP WB Rabbit IgG AA 59-239 Log in to see Polyclonal
1 ABIN1832268 DB ICC Mouse IgG1 Log in to see 880C3a
1 ABIN1924803 ICC ELISA WB Alkaline Phosphatase (AP) Mouse IgG2a AA 411-510 Log in to see
1 ABIN1924804 ICC ELISA WB APC Mouse IgG2a AA 411-510 Log in to see
1 ABIN1924805 ICC ELISA WB Biotin Mouse IgG2a AA 411-510 Log in to see
1 ABIN1924806 ICC ELISA WB FITC Mouse IgG2a AA 411-510 Log in to see
1 ABIN1924807 ICC ELISA WB PE Mouse IgG2a AA 411-510 Log in to see
1 ABIN1924808 ICC ELISA WB HRP Mouse IgG2a AA 411-510 Log in to see
1 ABIN2581142 ICC IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN931267 DB ICC Mouse IgG1 Log in to see 880C3a
1 ABIN1818428 ICC FACS IP WB Mouse IgG1 Log in to see 1B9
1 ABIN1818566 ICC FACS Rabbit IgG Log in to see Polyclonal
1 ABIN2581141 DB ICC WB Mouse IgG1 Log in to see 880C3a
1 ABIN1175608 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal
1 ABIN325550 ICC IF WB Rabbit Log in to see Polyclonal

Top referenced anti-PML antibody for Immunocytochemistry

Similar anti-PML Antibodies

Application / Reactivity Mouse (Murine) Human
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies
ELISA 3 Antibodies 44 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Gel Shift (GS) 1 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 29 Antibodies
Dot Blot (DB) 3 Antibodies
ELISA (Capture) 1 Antibodies

General

Antigen Promyelocytic Leukemia (PML) Antibodies
Epitope AA 375-425
(15), (14), (8), (7), (7), (6), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(115), (28), (11), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(93), (39)
Clonality
Polyclonal
Conjugate This PML antibody is un-conjugated
(5), (5), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
(115), (46), (42), (40), (25), (22), (16), (6), (5), (5), (3), (3), (2), (2), (1), (1), (1)
Pubmed 20 references available
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Product Details anti-PML Antibody

Target Details PML Application Details Handling References for anti-PML Antibody (ABIN252967) Images
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residue 375 and 425 of promyelocytic leukemia using the numbering given in entry NP_150241.2

Target Details PML

Product Details anti-PML Antibody Application Details Handling References for anti-PML Antibody (ABIN252967) Images back to top
Antigen
Alternative Name PML Protein (PML Antibody Abstract)
Background Gene Symbol: PML
Gene ID 5371
UniProt P29590
Research Area Transcription Factors, Cancer
Pathways p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus

Application Details

Product Details anti-PML Antibody Target Details PML Handling References for anti-PML Antibody (ABIN252967) Images back to top
Application Notes Western Blot 1:2000-1:10000, Immunohistochemistry 1:200-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation 2-5 μg/mg lysate, Immunohistochemistry-Paraffin 1:200-1:1000, Proximity Ligation Assay 1:200-1:5000Immunohistochemistry 1:200 to 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-PML Antibody Target Details PML Application Details References for anti-PML Antibody (ABIN252967) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-PML Antibody (ABIN252967)

Product Details anti-PML Antibody Target Details PML Application Details Handling Images back to top
Product cited in:

Berscheminski, Brun, Speiseder, Wimmer, Ip, Terzic, Dobner, Schreiner: "Sp100A is a tumor suppressor that activates p53-dependent transcription and counteracts E1A/E1B-55K-mediated transformation." in: Oncogene, Vol. 35, Issue 24, pp. 3178-89, 2016

Komatsu, Nagata, Wodrich: "An Adenovirus DNA Replication Factor, but Not Incoming Genome Complexes, Targets PML Nuclear Bodies." in: Journal of virology, Vol. 90, Issue 3, pp. 1657-67, 2016

Yao, Wu, Chen, Zhang, Chen, Zhang: "The Arginine/Lysine-Rich Element within the DNA-Binding Domain Is Essential for Nuclear Localization and Function of the Intracellular Pathogen Resistance 1." in: PLoS ONE, Vol. 11, Issue 9, pp. e0162832, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Wimmer, Berscheminski, Blanchette, Groitl, Branton, Hay, Dobner, Schreiner: "PML isoforms IV and V contribute to adenovirus-mediated oncogenic transformation by functionally inhibiting the tumor-suppressor p53." in: Oncogene, Vol. 35, Issue 1, pp. 69-82, 2016 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Chen, Hu, Chen, Lin, Liu, Lin, Lang, Yen, Chen: "KLHL39 suppresses colon cancer metastasis by blocking KLHL20-mediated PML and DAPK ubiquitination." in: Oncogene, Vol. 34, Issue 40, pp. 5141-51, 2015 Further details: Western Blotting

Bürck, Mund, Berscheminski, Kieweg, Müncheberg, Dobner, Schreiner: "KAP1 Is a Host Restriction Factor That Promotes Human Adenovirus E1B-55K SUMO Modification." in: Journal of virology, Vol. 90, Issue 2, pp. 930-46, 2015

Palibrk, Lång, Lång, Schink, Rowe, Bøe: "Promyelocytic leukemia bodies tether to early endosomes during mitosis." in: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 11, pp. 1749-55, 2014 (Sample species: Human).

Berscheminski, Wimmer, Brun, Ip, Groitl, Horlacher, Jaffray, Hay, Dobner, Schreiner: "Sp100 isoform-specific regulation of human adenovirus 5 gene expression." in: Journal of virology, Vol. 88, Issue 11, pp. 6076-92, 2014 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Coltella, Percio, Valsecchi, Cuttano, Guarnerio, Ponzoni, Pandolfi, Melillo, Pattini, Bernardi: "HIF factors cooperate with PML-RAR? to promote acute promyelocytic leukemia progression and relapse." in: EMBO molecular medicine, Vol. 6, Issue 5, pp. 640-50, 2014 (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Schreiner, Bürck, Glass, Groitl, Wimmer, Kinkley, Mund, Everett, Dobner: "Control of human adenovirus type 5 gene expression by cellular Daxx/ATRX chromatin-associated complexes." in: Nucleic acids research, Vol. 41, Issue 6, pp. 3532-50, 2013 (Sample species: Human). Further details: Western Blotting

Sivachandran, Wang, Frappier: "Functions of the Epstein-Barr virus EBNA1 protein in viral reactivation and lytic infection." in: Journal of virology, Vol. 86, Issue 11, pp. 6146-58, 2012

Chiantore, Vannucchi, Accardi, Tommasino, Percario, Vaccari, Affabris, Fiorucci, Romeo: "Interferon-β induces cellular senescence in cutaneous human papilloma virus-transformed human keratinocytes by affecting p53 transactivating activity." in: PLoS ONE, Vol. 7, Issue 5, pp. e36909, 2012

Bade, Nickels, Keusekotten, Praefcke: "Covalent protein modification with ISG15 via a conserved cysteine in the hinge region." in: PLoS ONE, Vol. 7, Issue 6, pp. e38294, 2012

Carracedo, Weiss, Leliaert, Bhasin, de Boer, Laurent, Adams, Sundvall, Song, Ito, Finley, Egia, Libermann, Gerhart-Hines, Puigserver, Haigis, Maratos-Flier, Richardson, Schafer, Pandolfi: "A metabolic prosurvival role for PML in breast cancer." in: The Journal of clinical investigation, Vol. 122, Issue 9, pp. 3088-100, 2012

Berscheminski, Groitl, Dobner, Wimmer, Schreiner: "The adenoviral oncogene E1A-13S interacts with a specific isoform of the tumor suppressor PML to enhance viral transcription." in: Journal of virology, Vol. 87, Issue 2, pp. 965-77, 2012 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Lee, Smart, Rubinstein, Low, Tse: "Reciprocal regulation of TREK-1 channels by arachidonic acid and CRH in mouse corticotropes." in: Endocrinology, Vol. 152, Issue 5, pp. 1901-10, 2011 (Sample species: Mouse (Murine)).

Jung, Finnen, Neron, Banfield: "The alphaherpesvirus serine/threonine kinase us3 disrupts promyelocytic leukemia protein nuclear bodies." in: Journal of virology, Vol. 85, Issue 11, pp. 5301-11, 2011

Everett: "Study of early events during herpes simplex virus type 1 infection by confocal microscopy." in: Methods (San Diego, Calif.), Vol. 55, Issue 2, pp. 144-52, 2011

Sivachandran, Dawson, Young, Liu, Middeldorp, Frappier: "Contributions of the Epstein-Barr virus EBNA1 protein to gastric carcinoma." in: Journal of virology, Vol. 86, Issue 1, pp. 60-8, 2011

Wimmer, Schreiner, Everett, Sirma, Groitl, Dobner: "SUMO modification of E1B-55K oncoprotein regulates isoform-specific binding to the tumour suppressor protein PML." in: Oncogene, Vol. 29, Issue 40, pp. 5511-22, 2010

Images

Product Details anti-PML Antibody Target Details PML Application Details Handling References for anti-PML Antibody (ABIN252967) back to top
Supplier Images
Western Blotting (WB) image for anti-PML antibody (Promyelocytic Leukemia) (AA 375-425) (ABIN252967) Western Blot and Immunoprecipitation: PML Antibody [ABIN252967] - Detection of Human ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PML antibody (Promyelocytic Leukemia) (AA 375-425) (ABIN252967) Immunohistochemistry-Paraffin: PML Protein Antibody [ABIN2529677] - FFPE section of h...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PML antibody (Promyelocytic Leukemia) (AA 375-425) (ABIN252967) Immunohistochemistry-Paraffin: PML Protein Antibody [ABIN2529677] - Section of human ...
Proximity Ligation Assay (PLA) image for anti-PML antibody (Promyelocytic Leukemia) (AA 375-425) (ABIN252967) Proximity Ligation Assay: PML Protein Antibody [ABIN2529677] - Secondary-conjugate Du...
Proximity Ligation Assay (PLA) image for anti-PML antibody (Promyelocytic Leukemia) (AA 375-425) (ABIN252967) Proximity Ligation Assay: PML Protein Antibody [ABIN2529677] - Secondary-conjugate Du...
Proximity Ligation Assay (PLA) image for anti-PML antibody (Promyelocytic Leukemia) (AA 375-425) (ABIN252967) Proximity Ligation Assay: PML Protein Antibody [ABIN2529677] - Secondary-conjugate Du...