anti-Rat (Rattus) PML antibody for Western Blotting

Recommended PML Antibody (supplied by: Log in to see )

Antigen
Promyelocytic Leukemia (PML) Antibodies
  • PML
  • MYL
  • PP8675
  • RNF71
  • TRIM19
  • 1200009E24Rik
  • AI661194
  • Trim19
  • RGD1562602
  • promyelocytic leukemia
  • PML
  • Pml
Reactivity
Human, Mouse (Murine), Rat (Rattus)
138
43
11
2
2
2
1
1
1
1
Host
Rabbit
113
41
1
Clonality
Polyclonal
Conjugate
This PML antibody is un-conjugated
7
6
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
135
59
58
45
43
38
17
16
7
6
5
4
3
3
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2457998
$ 433.13
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3487515 ABIN3021659 IHC WB Rabbit IgG Log in to see Polyclonal
3.3487515 ABIN2476174 IF WB Mouse IgG2a AA 411-510 Log in to see 1D12
3.3487515 ABIN927422 WB Rabbit C-Term Log in to see Polyclonal
3.3487515 ABIN5545023 WB Rabbit IgG Log in to see Polyclonal
1 ABIN2777721 IHC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN4886699 IHC (p) WB Rabbit IgG AA 141-179, N-Term Log in to see Polyclonal
1 ABIN462187 IHC (p) WB Rabbit IgG AA 588-637 Log in to see Polyclonal
1 ABIN1828620 IF ELISA WB Mouse IgG2a, kappa AA 411-510 Log in to see 1D12
1 ABIN2894095 IHC WB Rabbit Log in to see Polyclonal
1 ABIN1994884 IHC WB Rabbit IgG Log in to see
1 ABIN2920703 IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-PML Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies 4 Antibodies
In Vivo Imaging (IVI) 1 Antibodies
Western Blotting (WB) 43 Antibodies 119 Antibodies 12 Antibodies
Proximity Ligation Assay (PLA) 4 Antibodies 1 Antibodies
Immunoprecipitation (IP) 8 Antibodies 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies 20 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 55 Antibodies 25 Antibodies 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 16 Antibodies 14 Antibodies

General

Antigen Promyelocytic Leukemia (PML) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(138), (43), (11), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(113), (41), (1)
Clonality
Polyclonal
Conjugate This PML antibody is un-conjugated
(7), (6), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(135), (59), (58), (45), (43), (38), (17), (16), (7), (6), (5), (4), (3), (3), (1), (1), (1), (1)
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Product Details anti-PML Antibody

Target Details PML Application Details Handling Images
Purification Antibody is purified by peptide affinity chromatography method.
Immunogen Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PML.

Target Details PML

Product Details anti-PML Antibody Application Details Handling Images back to top
Antigen
Alternative Name PML (PML Antibody Abstract)
Background PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. PML localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions, all variants encode the same N-terminus.
Molecular Weight 59 kDa
Gene ID 5371
UniProt A4VCI9
Research Area Cancer
Pathways p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus

Application Details

Product Details anti-PML Antibody Target Details PML Handling Images back to top
Application Notes PML antibody can be used for detection of PML by ELISA at 1:312500. PML antibody can be used for detection of PML by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions For Research Use only

Handling

Product Details anti-PML Antibody Target Details PML Application Details Images back to top
Format Lyophilized
Reconstitution Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration 1 mg/mL
Buffer Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice As with any antibody avoid repeat freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment For short periods of storage (days) store at 4 °C. For longer periods of storage, store PML antibody at -20 °C.

Images

Product Details anti-PML Antibody Target Details PML Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PML antibody (Promyelocytic Leukemia) (ABIN2457998) anti-Promyelocytic Leukemia (PML) antibody